ライフサイエンスコーパス: transgenesis

1 or intracytoplasmic sperm injection-mediated transgenesis.

2 We have developed a unique method for mouse transgenesis.

3 cterial artificial chromosome (BAC)-mediated transgenesis.

4 its of plasmids in phiC31 integrase-mediated transgenesis.

5 Drosophila and to any organism conducive to transgenesis.

6 ncies of conventional random mutagenesis and transgenesis.

7 fer and the technical challenges of germline transgenesis.

8 bineering, and bacteriophage PhiC31-mediated transgenesis.

9 sibility of using it routinely for mammalian transgenesis.

10 e (RNAi) "knockdown" mice through lentiviral transgenesis.

11 an important goal in gene therapy and animal transgenesis.

12 ve form of the TH receptor by sperm-mediated transgenesis.

13 al stem cells using Cre-recombinase-mediated transgenesis.

14 requirement for a selectable marker in plant transgenesis.

15 aced with respect to enhancing yield through transgenesis.

16 itches were engineered into mouse hearts via transgenesis.

17 ted the infarct-sparing effect of PKCepsilon transgenesis.

18 d Mrf4 using bacterial artificial chromosome transgenesis.

19 ransient transfection of Xenopus embryos and transgenesis.

20 lements have been defined, generally through transgenesis.

21 ovides an alternative means of cell-mediated transgenesis.

22 of TnI(146Gly) was not simply an artifact of transgenesis.

23 were similar and independent of the mode of transgenesis.

24 in GluR5, using embryonic stem cell-mediated transgenesis.

25 ermatozoa and suggest an adaptable method of transgenesis.

26 as a model for naturally occurring recursive transgenesis.

27 to be difficult to achieve with conventional transgenesis.

28 embryonic stem cells and blastocyst-mediated transgenesis.

29 ds doubt on the usefulness of Fugu genes for transgenesis.

30 ructural rearrangements that can result from transgenesis.

31 Neither CRISPR/Cas9 nor TALEN increased BAC transgenesis.

32 A B-CLL/lymphoma incidence, enhanced by TCL1 transgenesis.

33 beling and manipulation without the need for transgenesis.

34 ism have emerged as an attractive system for transgenesis.

35 detection of enhancer activity in transient transgenesis.

36 e sperm, have material advantages for use in transgenesis.

37 eukaryotes that are not amenable to germline transgenesis.

38 recombination almost as easy as conventional transgenesis.

39 eristic makes piggyBac useful for reversible transgenesis, a potentially valuable feature when genera

40 By ChIP and transgenesis analysis we show that the Gbx2 regulatory e

41 fish and mouse muscle by transposon-mediated transgenesis and adeno-associated viral vectors, respect

42 t proteins was identified through the use of transgenesis and computational analysis.

43 by bacterial artificial chromosome-mediated transgenesis and demonstrated complete rescue of spina b

44 Transgenesis and double labeling of NTS and HCRT neurons

45 Finally, transgenesis and electroporation are discussed as potent

46 es can be expected to have practical uses in transgenesis and epidemiological studies.

47 ta indicate that SB is an efficient tool for transgenesis and expression in zebrafish, and that the t

48 SB transposase enhanced the transgenesis and expression rate sixfold (from 5 to 31%)

49 ulatory properties using zebrafish transient transgenesis and found that 10 (63%) strongly modulate t

50 a tractable pathway forward toward germline transgenesis and functional genomics of parasitic helmin

51 velop an animal model system using zebrafish transgenesis and gene targeting to provide an explanatio

52 loped an animal model system using zebrafish transgenesis and gene targeting to provide an explanatio

53 Selectable markers are widely used in transgenesis and genome editing for selecting engineered

54 eran insects for many applications including transgenesis and genome editing.

55 essing different reporters together with BAC transgenesis and immunohistochemistry.

56 nsposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens

57 We also provide strategies for Tol2-based transgenesis and large-scale husbandry conditions that a

58 ransposable element Sleeping Beauty (SB) for transgenesis and long-term expression studies in the zeb

59 ide-ranging applications, such as reversible transgenesis and modified targeting of insertions.

60 month) and can be adapted for other types of transgenesis and mRNA injections in Drosophila.

61 Approaches employing transgenesis and mutagenesis now permit investigation of

62 amilies may add to the limited repertoire of transgenesis and mutagenesis tools for a wide range of o

63 omising resource for cell engineering, mouse transgenesis and pre-clinical gene therapy studies.

64 resented here will greatly facilitate murine transgenesis and precise structure/function dissection o

65 Here, we employ BAC transgenesis and quantitative imaging methods to investi

66 nome projects and the nascent application of transgenesis and RNA interference technologies.

67 Using germ-line transgenesis and somatic cell gene transfer methods, we

68 soma mansoni, appraises delivery systems for transgenesis and stable gene silencing, considers ways o

69 ribe a protocol for high-efficiency germline transgenesis and sustained transgene expression in pigs

70 ribe a protocol for high-efficiency germline transgenesis and sustained transgene expression in rabbi

71 ribe a protocol for high-efficiency germline transgenesis and sustained transgene expression in two i

72 viral strategy by combining transposon-based transgenesis and the clustered regularly interspaced sho

73 gastrointestinal phases on the basis of the transgenesis and the cultivation periods.

74 y applicable to all model systems that allow transgenesis and will make it possible to determine epig

75 Primates pose unique challenges to transgenesis and, although this field is still in its in

76 ntisense morpholinos, small molecule screen, transgenesis, and cell transplantation as related to blo

77 netics, well-established high efficiency for transgenesis, and ease of transplantation, further explo

78 ic Cre lines: conventional transgenesis, BAC transgenesis, and gene knock-in.

79 e delivery such as cell transfection, animal transgenesis, and gene therapy.

80 of the Xenopus laevis Xsox17alpha(1) gene by transgenesis, and have identified two important control

81 and advantages compared with I-SceI-mediated transgenesis, and it can result in more than 30% of anim

82 on of fluorescent in situ hybridization, BAC transgenesis, and knockdown experiments reveals that per

83 d whole-body connectomics, activity imaging, transgenesis, and neuron ablation to characterize the ci

84 embryonic stem (ES) cells, novel methods for transgenesis, and the completion of the first draft of t

85 ney bee development, genetics, pathogenesis, transgenesis, and toxicology.

86 developed a bacterial artificial chromosome transgenesis approach that allowed the expression of myo

87 Using an interspecies transgenesis approach, we find functional conservation b

88 tic silencing in vitro and conditional mouse transgenesis approaches in vivo to demonstrate that neur

89 developmental biology, stem cell biology and transgenesis are rapidly emerging.

90 e efficient methods such as gene cloning and transgenesis are required to deploy resistance genes.

91 sgenes in the context of lentivirus-mediated transgenesis as well as CRISPR-Cas-mediated knock-ins.

92 We test >1,000 putative enhancers by transgenesis assays in zebrafish to ascertain the identi

93 We performed in vivo mosaic transgenesis assays in zebrafish to quantitatively test

94 ZFNs enable targeted transgenesis at a frequency of up to 15% following trans

95 Transgenesis at three locations is closely associated wi

96 e inner ear-specific Cre lines: conventional transgenesis, BAC transgenesis, and gene knock-in.

97 Gene knockdown approaches and transgenesis-based lineage and functional analyses have

98 To compare the two modes of transgenesis, both vectors used the Xenopus translationa

99 t transposon system improved stable cellular transgenesis by 40-fold, has an apparent preference for

100 We propose that an approach of combining transgenesis by lentiviral vectors expressing siRNAs can

101 Finally, these studies show that YAC transgenesis can be a useful tool in functional analysis

102 whether comparative genome analysis and rat transgenesis can be used to identify functional regulato

103 ation of DNA fragments into the host genome, transgenesis can lead to insertional mutagenesis if a co

104 e extended this work and now report that mII transgenesis can readily be applied to a range of larger

105 Using transgenesis, cardiac-specific overexpression of protein

106 rythroid precursors by proviral insertion or transgenesis causes erythroleukemias in mice.

107 immunogenetic background, we created somatic transgenesis CNS models of tauopathy utilizing neonatal

108 Transposon-mediated transgenesis compares favorably in terms of both efficie

109 Transposon-mediated transgenesis compares favorably in terms of both efficie

110 Transgenesis efficiency is approximately 15%; most anima

111 Genetic screens, germline transgenesis, electroporation of plasmid DNA, and microi

112 cross-model standardization of commonly used transgenesis elements, streamlines DNA construct creatio

113 s of biological systems, the ability to move transgenesis experiments efficiently between organisms b

114 In the transgenesis experiments, HCMV YACs and derivatives gene

115 By using Tol2-mediated transgenesis, experiments can be performed within weeks

116 compatible selectable markers, promoters and transgenesis facilitation sequences.

117 is work emphasizes the utility of Drosophila transgenesis for elucidating the precise mechanisms of C

118 e-wide ChIP analysis combined with zebrafish transgenesis for identifying long-range transcriptional

119 ndicate successful F5H-amiRNA and C3H-amiRNA transgenesis for lignin reduction in jute.

120 We have developed an alternative to transgenesis for producing antigen-specific T cells in v

121 The method establishes gamete-targeted transgenesis for the first time in a carnivore.

122 inducible gene expression systems supplement transgenesis for the study of X. laevis metamorphosis, o

123 lentiviral system that simplifies fibroblast transgenesis for the two pioneer transcription factors,

124 fection provides both an alternative mode of transgenesis for zebrafish work and a possible means of

125 ion of modern genetic manipulations, such as transgenesis, gene ablation (knockouts) and targeted mut

126 auty (SB) transposon is an emerging tool for transgenesis, gene discovery, and therapeutic gene deliv

127 eous multiplexed genome modification, animal transgenesis, gene transfer in vivo achieving long-term

128 on of NCLX in the mouse heart by conditional transgenesis had the beneficial effect of augmenting mCa

129 Transgenesis has also been applied in nonrodent vertebra

130 Transgenesis has been a mainstay of mouse genetics for o

131 his lack of sequence identity, cross-species transgenesis has identified some cases where non-coding

132 cterial artificial chromosome (BAC) mediated transgenesis has proven to be a highly reliable way to o

133 Mouse transgenesis has provided fundamental insights into panc

134 tion of the cardiac protein complement using transgenesis, has begun to provide mouse models of cardi

135 tation using bacterial artificial chromosome transgenesis implicated zinc finger BTB-POZ domain prote

136 The combination of Fugu genome analysis and transgenesis in a mammal is a powerful tool for identify

137 We used cardiac-targeted transgenesis in a mouse model to delineate mechanisms ac

138 inciple that underlies ISSI is applicable to transgenesis in all organisms.

139 as potential for development as a vector for transgenesis in avian species.

140 Recent developments in RNA interference and transgenesis in birds should facilitate the development

141 ransgenesis resource is also compatible with transgenesis in Drosophila, zebrafish and mammalian cell

142 ng, the evaluation of stem cell therapy, and transgenesis in ferret models of human disease.

143 contemporary models of SSc vascular disease, transgenesis in fibrocyte research, the contribution to

144 Using transgenesis in frog embryos, we have identified an 82 b

145 , gene transfer in isolated rat myocytes and transgenesis in mice, to ascertain whether parvalbumin (

146 ite of recent progress in the development of transgenesis in parasitic nematodes, several impediments

147 , we introduce a novel technology to perform transgenesis in quail, based on the in vivo transfection

148 Thus, an efficient method for BAC transgenesis in rats would be valuable.

149 e compared PhiC31-based integration and Tol2 transgenesis in the analysis of the activity of a novel

150 We established stable transgenesis in the annelid Platynereis, a reference spe

151 tems offer simple, yet robust, platforms for transgenesis in the frog.

152 We have used BAC transgenesis in the mouse to begin to delineate the regi

153 Using loss-of-function genetics and transgenesis in the mouse, we show here a strong genetic

154 Recently published studies on transgenesis in the necromenic nematode Pristionchus pac

155 ations and homologous recombination-mediated transgenesis in the sea anemone Nematostella vectensis.

156 Although transgenesis in the zebra finch has been accomplished, i

157 more, making use of the ability to carry out transgenesis in X. laevis and gene knockdown in X. tropi

158 developed a simple approach for large-scale transgenesis in Xenopus laevis embryos and have used thi

159 n an in vivo context using non-viral somatic transgenesis in Xenopus tadpole tail muscle, a setting t

160 periments demonstrate the power of transient transgenesis in zebrafish to efficiently define cis-acti

161 Here, we use time-lapse imaging and transgenesis in zebrafish to visualize the role of synap

162 nscriptional enhancer using Tol2-mediated G0 transgenesis in zebrafish.

163 eering, mutagenesis screens, and vectors for transgenesis including gene therapy.

164 would be desirable for many applications in transgenesis, including gene therapy.

165 PhiC31-mediated transgenesis integrates these large DNA fragments at spe

166 ave incorporated the four common elements of transgenesis into a modular, recombination-based cloning

167 Zebrafish transgenesis is a powerful and increasingly common strat

168 sgenic insects, and while powerful, mosquito transgenesis is a resource- and time-intensive technique

169 Yeast artificial chromosome (YAC) transgenesis is associated with a high frequency of dele

170 Zebrafish transgenesis is increasingly popular owing to the optica

171 In addition, transgenesis is most often needed to confirm gene functi

172 A practical capability for cat transgenesis is needed to realize the distinctive potent

173 tic screens in any higher eukaryote in which transgenesis is possible.

174 heir native chromosomal locations, classical transgenesis is still the preferred experimental approac

175 tome network analysis in organisms for which transgenesis is straightforward.

176 As a flatworm amenable to transgenesis, it complements the historically used plana

177 By use of gene targeting and/or transgenesis, it is now possible to make defined changes

178 Utilizing transgenesis, lineage tracing, flow cytometry, and immun

179 fluorescent protein (DsRed) were also useful transgenesis markers, indicating that multiple reporters

180 MO1 or LMO2 by chromosomal translocations or transgenesis may displace Lmo4 from this complex and the

181 ral nervous system by means of somatic brain transgenesis mediated by adeno-associated virus.

182 The reported transgenesis method and annotated genome sequence will p

183 Here we report development of a transgenesis method for Macrostomum lignano, a basal fla

184 report we describe an easy, highly efficient transgenesis method for Xenopus.

185 This technique has promise as a germ-line transgenesis method in other vertebrate species and for

186 ic injections, offering an easy-to-implement transgenesis method to the scientific community.

187 otocol for a microinjection-based transposon transgenesis method using a 'natural breeding' strategy

188 However, conventional transgenesis methods are plagued by position effects: th

189 However, the lack of transgenesis methods considerably hampers their wider us

190 In situ transgenesis methods such as viruses and electroporation

191 ith Agrobacterium tumefaciens, whereas other transgenesis methods, such as gold particle-mediated tra

192 somes over standard, plasmid injection-based transgenesis methods.

193 e II (mII) oocytes could efficiently promote transgenesis (mII transgenesis) when coinjected with spe

194 try, and electron microscopy correlated with transgenesis, mitochondrial structure, and biogenesis.

195 ression of EcSOD by somatic gene transfer or transgenesis (muscle creatine kinase [MCK]-EcSOD) in mic

196 a tool in mammalian experimental systems for transgenesis, mutagenesis, and genome engineering.

197 d Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the m

198 Transgenesis of a construct containing human A1- and H-t

199 he sks mutant were completely rescued by the transgenesis of a genomic fragment containing the wild-t

200 nipulation, VCre recombinase expression, and transgenesis of human cells.

201 ol details how to prepare intact YAC DNA for transgenesis of mice and involves separation of YAC DNA

202 ust growth advantage relative to S. bayanus; transgenesis of S. cerevisiae GAL promoter alleles or GA

203 cate the utility of VSVG-pseudotyped MLV for transgenesis of S. mansoni, herald a tractable pathway f

204 roach demonstrated definitively that somatic transgenesis of schistosome chromosomes had taken place

205 as not been reported but transposon-mediated transgenesis of schistosomes might supersede current met

206 Here, by using endothelial cell-specific transgenesis of the caveolin-1 (Cav-1) gene in mice, we

207 rmined and the virus can be reconstituted by transgenesis of the host.

208 By using endothelial cell (EC)-specific transgenesis of the mitochondrial form of the thioredoxi

209 somal integration of a transgene and somatic transgenesis of this important human pathogen, in this i

210 Sperm-mediated transgenesis of Xenopus laevis is the first application

211 The conditional BAC transgenesis offers a novel strategy to model CNVs with

212 The impact of transgenesis on profiles was much less than that of tiss

213 Here we examine the impact of transgenesis on the fitness of Aedes aegypti, a mosquito

214 d on genetically-engineered strains made via transgenesis or gene targeting in embryonic stem cells.

215 pitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic

216 human hematopoiesis and immune responses by transgenesis or knockin of human genes.

217 ls and (2) augmenting mPFC RELN levels using transgenesis or prefrontal pharmacology prevents the pHF

218 IL-7Ralpha transgenesis or T-cell-specific ablation of Gfi-1 restor

219 Thus, ectopic expression of Lmo2 by transgenesis, or by chromosomal translocations in humans

220 isoform in heart via tetracycline-inducible transgenesis, or CRISPR/Cas9-mediated genome editing, re

221 aboratory (C57BL/6, 129, CD-1) mice used for transgenesis, pharmacology, and toxicology studies.

222 We describe a transgenesis platform for Drosophila melanogaster that i

223 This transgenesis platform should greatly facilitate structur

224 Transgenesis promises a powerful means for assessing gen

225 Transgenesis provides a means of stably modifying the ma

226 Tol2-mediated transgenesis provides different features and advantages

227 The transgenesis reaction and egg injection take one morning

228 Recent advances in molecular biology and transgenesis research have renewed interest in genetical

229 PKCbetaII in the colon of PKCbetaKO mice by transgenesis restores susceptibility to AOM-induced colo

230 re efficient approach than the classical BAC transgenesis, resulting in complete BAC integration with

231 LFG expression generated by shRNA lentiviral transgenesis (shLFG mice) as well as LFG null mice.

232 A novel mosaic transgenesis strategy reveals both developmental compart

233 In plant transgenesis studies, the ZmGSTUs did not perturb porphy

234 L54 promoter is activated in murine cells, a transgenesis system based on yeast artificial chromosome

235 c and adult mice including those produced by transgenesis, targeted mutagenesis and chemical mutagene

236 The frog transgenesis technique ultimately promises to make mutag

237 says and zebrafish green fluorescent protein transgenesis tested conserved elements for transcription

238 , low-cost system for stable in vivo somatic transgenesis that allows for individual cells to be gene

239 previously demonstrated via cardiac-specific transgenesis that modest increases in normal CryAB are n

240 ryonic stem cells is commonly used for mouse transgenesis to achieve ubiquitous and persistent transg

241 come together, from yeast and Drosophila fly transgenesis to CI diversity patterns in natural mosquit

242 These data demonstrate the ability of mII transgenesis to deliver large tgs efficiently.

243 To begin to explore these issues, we used transgenesis to determine the feasibility of effecting a

244 We employed transgenesis to either overexpress or knock down Drosoph

245 th TRPC3 expression in the mouse heart using transgenesis to examine the potential role of store-oper

246 We used transgenesis to explore the requirement for downregulati

247 AND We used bacterial artificial chromosome transgenesis to generate a mouse model with increased gh

248 We used lentiviral transgenesis to generate NOD mice in which IL-17 is sile

249 n with human cytokines and growth factors by transgenesis to improve human cell reconstitution and th

250 ome engineering and P1 artificial chromosome transgenesis to localize the haploinsufficient gene in t

251 B cells constrained by transgenesis to produce an Ag receptor derived from a co

252 Here, we utilized transgenesis to provide evidence suggesting that the bas

253 Using transgenesis to replace endogenous mouse proteins with h

254 Using transgenesis to replace mouse ZP2 and/or ZP3 with human

255 We have used transgenesis to replace the ventricular isoform of the e

256 FP ribosomal tag (expressed virally or using transgenesis) to immunoprecipitate translating mRNAs fro

257 netic techniques, such as gene targeting and transgenesis, to study cognitive function in adult anima

258 xpression of cGi500 were generated by random transgenesis using an SM22alpha promoter fragment or by

259 Transgenesis using cardiac-specific expression has been

260 s by a single i.p. injection and for axolotl transgenesis using I-SceI meganuclease and the mini Tol2

261 DNA constructions used in transgenesis usually contain four elements, including se

262 important, this is also the first report of transgenesis via germ cell transplantation in a nonroden

263 nerating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange

264 The efficiency of large-construct mII transgenesis was at least as high as that with small con

265 proach of transient embryo transfections and transgenesis was used to locate transcriptional control

266 Yeast artificial chromosome-based transgenesis was used to overexpress a disease-associate

267 Lentiviral-mediated transgenesis was used to produce rats expressing a short

268 e PKD function at these different locations, transgenesis was used to target active PKD either to the

269 tile mouse model of tauopathy, somatic brain transgenesis was utilized to deliver adeno-associated vi

270 BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that lab

271 Using bacterial artificial chromosome (BAC) transgenesis we have targeted expression of a neurotoxic

272 Finally, using zebrafish transgenesis, we also demonstrate that RET -49.8 directs

273 Using BAC recombination and transgenesis, we characterized the expression of human s

274 Using BAC transgenesis, we created a mouse model with a humanized

275 t using BAC recombineering and site-directed transgenesis, we demonstrate that the two CRMs are not r

276 Using transgenesis, we effected a cardiac-specific replacement

277 Using lentiviral transgenesis, we generated NOD mice in which Slc11a1 is

278 Utilizing BAC transgenesis, we generated transgenic zebrafish in which

279 Using bioinformatics and Tol2-mediated transgenesis, we have generated zebrafish transgenic rep

280 Using cardiac-specific transgenesis, we have now obtained major myosin isoform

281 ne 10 tetramers and RNAi loss of function by transgenesis, we identified a large class Ib-dependent C

282 Using frog transgenesis, we identify enhancer elements driving expr

283 through a combination of transcriptomics and transgenesis, we identify sestrins, a family of stress-i

284 Using a combination of zebrafish and mouse transgenesis, we screened 15 conserved non-coding sequen

285 Moreover, using transgenesis, we show that interference in this mechanis

286 ers and RNA interference loss of function by transgenesis, we show that XNC10-restricted iValpha6 T c

287 iting of primary human progenitors and mouse transgenesis, we validate the BCL11A erythroid enhancer

288 tochemical light and electron microscopy and transgenesis were used to study the subcellular distribu

289 could efficiently promote transgenesis (mII transgenesis) when coinjected with sperm and small (<5 k

290 umvents the drawbacks of transposon-mediated transgenesis, where random transgene integration into th

291 enging applications of these developments is transgenesis, which allows for insertion of foreign DNA

292 ies of wild-type Na(+),K(+)-ATPase alpha3 by transgenesis, which also rescued Na(+),K(+)-ATPase activ

293 hniques, including gene therapy and germline transgenesis, will likely hasten the genetic progress to

294 Transgenesis with bacterial artificial chromosome (BAC)

295 Transgenesis with large DNA molecules such as yeast arti

296 We employed lentiviral-mediated transgenesis with promoterless TetO-containing gene trap

297 Germinal transgenesis with spg1-gfp led to stable medaka lines.

298 s and robustly predicted new CRM activity in transgenesis, with newly identified Twist-occupied regio

299 d zinc finger nucleases (ZFNs) for efficient transgenesis without drug selection into the PPP1R12C ge

300 e whether a gene trap approach combined with transgenesis would be appropriate for performing inserti