ライフサイエンスコーパス: transposon insertion

1 c regions showing significant enrichment for transposon insertion.

2 tein (CRP) was identified as the site of the transposon insertion.

3 ed-cut palindromic target site model for DNA transposon insertion.

4 n the first selection are homozygous for the transposon insertion.

5 ency production of clones harboring a single transposon insertion.

6 ertion sites identified 7 genes activated by transposon insertions.

7 selective advantage for cells with multiple transposon insertions.

8 ns, more than two-thirds of which are due to transposon insertions.

9 ed to remove what are likely the remnants of transposon insertions.

10 le-induced locomotion that are caused by the transposon insertions.

11 marks" at intergenic sites known to tolerate transposon insertions.

12 ation of genes in lines carrying Spm or dSpm transposon insertions.

13 e as a high-throughput technique for mapping transposon insertions.

14 sion loop of the E protein was intolerant of transposon insertions.

15 on such as recO, recQ, and a cis-acting pilE transposon insertion all rescue the RecA-dependent growt

16 Here we report a unique transposon insertion allele in a small ORF located immed

17 ascicular fibers, as previously seen in tnt1 transposon insertion alleles.

18 om the gene sequence that is duplicated upon transposon insertion, allowing perfect splicing out of t

19 n in ISA1, and isa2-339, which was caused by transposon insertion and conditioned loss of ISA2.

20 genomes, we describe a new method called the Transposon Insertion and Depletion AnaLyzer (TIDAL) and

21 Thus, transposon insertion and sequence co-option may explain

22 genic region, while FT2c and FT2d obtained a transposon insertion and structural rearrangement, respe

23 Rates of Mu transposon insertions and excisions are both high in lat

24 olanaceous R genes revealed the magnitude of transposon insertions and local duplications that result

25 relation of methylation-poor regions with Mu transposon insertions and recombination, and copy number

26 We show that IM-Fusion accurately identifies transposon insertions and their true target genes.

27 vrC, uvrD, and mfd) were each disrupted by a transposon insertion, and the uvrB and uvrD mutants were

28 ater fish express more GDF6 due in part to a transposon insertion, and transgenic overexpression of G

29 pair expansions of 2-15 bp repeat units, (4) transposon insertions, and (5) INDELs containing random

30 k1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneousl

31 l variants (SVs), including thousands of new transposon insertions, and of highly polymorphic and med

32 of sequence upstream of pilE were devoid of transposon insertions, and some deletions in these regio

33 Thus, a natural transposon insertion appears to mediate an ecologically

34 Recurrent sites of transposon insertion are commonly identified using ligat

35 The locations of transposon insertions are highly reproducible and agree

36 These 17 transposon insertions are located in nine open reading f

37 S regions, direct transcriptional effects of transposon insertions are observed.

38 sertion lines showed that the effects of the transposon insertions are often dependent on development

39 Maize lines lacking RAF1 due to Mutator transposon insertions are Rubisco deficient and seedling

40 with pigmentation distinct from the initial transposon insertions as a consequence of germline trans

41 llection of mutant strains containing single transposon insertions associated with different genes.

42 different pools were identified, each with a transposon insertion at a different position within the

43 dating the cooperation between JAK2V617F and transposon insertion at the Erg locus in the JAK2V617F-p

44 ng, and that the loss of silencing caused by transposon insertions at plant Pc-G targets reflects imp

45 An M. marinum strain bearing a transposon-insertion between the MMAR_1663 and MMAR_1664

46 he number and fraction of rDNA units without transposon insertions, but not with total rDNA locus siz

47 We aimed to retarget transposon insertions by comparing a series of novel hyp

48 We screened mutants with non-redundant transposon insertions by fluorescence-activated cell sor

49 However, genetic changes such as transposon insertions can also lead to changes in DNA me

50 Transposon insertions can be nested and make the task of

51 ntial PA14 genes are represented by a single transposon insertion chosen from a comprehensive library

52 Recurrent transposon insertions could be mapped to genes in the JA

53 ution in host genomes using multiple de novo transposon insertion datasets in both plants and animals

54 refinement in the interpretation of observed transposon insertions demonstrated that genes without in

55 ic datasets representing different levels of transposon insertion density and sequence coverage.

56 Evidence indicates that this transposon insertion, designated CRISPR3*, is a gain-of-

57 We previously reported that a transposon insertion disrupting rpoE resulted in the dec

58 Statistical analysis of transposon insertion distribution revealed a set of 453

59 wed Kolmogorov-Smirnov (K-S) test to analyze transposon insertion distributions in sequence windows o

60 -oriented approaches in recovering validated transposon insertion events.

61 A transposon insertion FlhDC(-) mutant produces very low l

62 ns, and gene essentiality calls were made by transposon insertion frequency analysis (TIFA).

63 Cloning of transposon insertions from lymphomas/leukemias identifie

64 Genetic screening using random transposon insertions has been a powerful tool for uncov

65 Analysis of over 16,000 transposon insertions identified 77 candidate CRC genes,

66 tion sites in these lesions, and analysis of transposon insertions identified candidate prostate canc

67 Analysis of transposon insertions identified eight genes including B

68 In this study, we identified a transposon insertion in a novel gene, designated disA, t

69 fied a noncytotoxic M. marinum strain with a transposon insertion in a predicted N-alpha-terminal ace

70 he leech, we found one mutant, JG752, with a transposon insertion in an ascU homolog, encoding an ess

71 ular interest, since this mutant contained a transposon insertion in an intergenic region between BAs

72 Transposon insertion in ccpA also restored proline proto

73 nonmotile mutant of C. jejuni 81-176 with a transposon insertion in Cj1026c, but verification of the

74 We previously found that a transposon insertion in Cjj81176_1038, encoding a homolo

75 We identify a transposon insertion in fhuB, a gene that encodes a ferr

76 tional fusion resulted in the isolation of a transposon insertion in glmS, encoding the essential enz

77 entified a multidrug-sensitive mutant with a transposon insertion in lpqB, the gene located immediate

78 This cluster was identified by a polar transposon insertion in orf2 that resulted in a strain d

79 AB307.27 contained its transposon insertion in pbpG, which encodes the putative

80 contrast to the case for this femAB mutant, transposon insertion in SAV2335, herein named lyrA (lyso

81 Erg) and Ets1 were the most common sites for transposon insertion in SB-induced JAK2V617F-positive er

82 rpels (foc), an Arabidopsis mutant with a Ds transposon insertion in the 3' regulatory region of MIR1

83 s well as rrp4 mutants with Piggy Bac (PBac) transposon insertion in the 3'UTR and RNAi flies, we det

84 Here, truncation of P65 due to transposon insertion in the corresponding gene resulted

85 1 mutant (bm1-das1) that contains a 3,444-bp transposon insertion in the first intron of CAD2 gene.

86 A transposon insertion in the middle of the coding sequenc

87 of maize knotted1, Kn1-DL, which contains a transposon insertion in the promoter in addition to a ta

88 mutagenesis-defective mutants, we isolated a transposon insertion in the rpoE P2 promoter.

89 ted expression potentially associated with a transposon insertion in the upstream intergenic region,

90 genetic variation including a Harbinger DNA transposon insertion in the upstream regulatory region o

91 of these transformants were shown to have a transposon insertion in the uspA1 gene.

92 In this study, we report that a transposon insertion in the waaL gene, encoding O-antige

93 Here we report that a Mu transposon insertion in the Zea mays (maize) gene encodi

94 Our lab recently isolated a transposon insertion in waaL, encoding O-antigen ligase,

95 utants allowed us to monitor the behavior of transposon insertions in 758 different gene loci.

96 servation and our ability to generate random transposon insertions in A. actinomycetemcomitans, we de

97 sequencing identified more than 200 de novo transposon insertions in alphabeta neurons, including in

98 M. truncatula lines harboring transposon insertions in CCR1 exhibit drastically reduce

99 Using transposon insertions in cloned katA DNA, we found that

100 Compared with creating new transposon insertions in each round, this method takes l

101 However, transposon insertions in either rpoN, truB, PA4068, PA40

102 Transposon insertions in gacA and gacS increased sliding

103 We describe phenotypes conditioned by transposon insertions in genes encoding the maize (Zea m

104 g technique whereby those mutants possessing transposon insertions in genes essential for in vivo sur

105 Three mutants, each of which harboured transposon insertions in genes for transmembrane protein

106 identified competence-defective mutants with transposon insertions in genes not previously implicated

107 ses with Drosophila strains carrying P[lacW] transposon insertions in genes without documented recomb

108 Transposon insertions in Geobacter sulfurreducens GSU150

109 Four mutants contained transposon insertions in gerHA, gerHB, gerHC, and pagA,

110 Many of the pgl mutants are produced by transposon insertions in glycosyltransferase genes.

111 characterize gliding-altered mutants having transposon insertions in MPN311, encoding the cytoskelet

112 we identified thousands of putative somatic transposon insertions in neurons from individual Drosoph

113 developed mammary tumors, most of which had transposon insertions in one of two RASGAP genes, neurof

114 to RNI resistance, we isolated mutants with transposon insertions in pafB and pafC.

115 Four of the mutants had transposon insertions in remA, which encodes a cell surf

116 ndril formation to spreading colonies, while transposon insertions in retS abolished motility.

117 acid-sensitive M. tuberculosis mutants with transposon insertions in Rv2136c, Rv2224c, ponA2, and ly

118 Transposon insertions in sprB resulted in cells that wer

119 Transposon insertions in the 269 growth-defective strain

120 Interestingly, transposon insertions in the epidermal growth factor rec

121 Transposon insertions in the only annotated Ser/Thr prot

122 Transposon insertions in the oprD gene led not only to c

123 ts in the H37Rv strain background containing transposon insertions in the rv0072, rv0405, and rv2958c

124 Three of these mutants had transposon insertions in the uspA2H gene, which encodes

125 l-dependent endopeptidase, acquired distinct transposon insertions in two independent mutants.

126 Mutant strains harbouring transposon insertions in two such genes, toxR (a toxin r

127 tectable in bacteria carrying an hpf::Himar1 transposon insertion, indicating that HPF is required fo

128 ct on specialized metabolite production of a transposon insertion into a Pseudomonas aeruginosa phena

129 unctiforme ATCC 29133 was obtained by random transposon insertion into open reading frame NpR1273.

130 oson mutant library, we showed that a single transposon insertion into the A. phagocytophilum dihydro

131 nut1 is caused by an Ac transposon insertion into the coding region of a unique

132 Rather, Tn5367 transposon insertion into the prrA promoter only decreas

133 Transposon insertions into genes encoding functions nece

134 plant genome sizes are largely explained by transposon insertions into heterochromatic regions.

135 teins are endowed with the ability to direct transposon insertions into the genome near to where they

136 The FT2c allele carrying a transposon insertion is nearly fixed in soybean landrace

137 encing approaches applied to the analyses of transposon insertion junction fragments generated in hig

138 le-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), which outperforms existin

139 genes through construction of complex random transposon insertion libraries and quantification of eac

140 Random transposon insertion libraries have proven invaluable in

141 sequencing affords an efficient analysis of transposon insertion libraries, which can be used to ide

142 hia coli, we used flow cytometry to screen a transposon insertion library and identified a wecE mutan

143 Using a highly saturated transposon insertion library combined with next-generati

144 o identify regulators of CyaB, we screened a transposon insertion library for mutants with reduced in

145 Phenotypic screening of a GBS transposon insertion library identified a mutation withi

146 However, our analyses of the high-density transposon insertion library in pESBL also revealed two

147 ly test this hypothesis, we used a bar-coded transposon insertion library in tandem with cell sorting

148 Here, we present a near-saturating transposon insertion library in Vibrio cholerae strain C

149 Screening of a transposon insertion library of A. baumannii ATCC 19606T

150 By screening a transposon insertion library of F. tularensis LVS in the

151 etter understand this process, we screened a transposon insertion library of the F. tularensis live v

152 and the genetics of this process, a mini-Tn5 transposon insertion library was constructed in strain E

153 A high-density transposon insertion library was grown under biofilm-ind

154 omparison of the genes disrupted in the PA14 transposon insertion library with an independently const

155 based on the assembly of a saturated Mariner transposon insertion library.

156 We have used a transposon insertion line to investigate the physiologic

157 Loss of function of CSE in transposon insertion lines of M. truncatula results in s

158 isolated from a population of Arabidopsis Ds transposon insertion lines.

159 ity defects were characterized further, with transposon insertions mapping to 32 different open readi

160 The paternal disruption of imprinting by transposon insertions may reflect a requirement for sequ

161 medulloblastoma dissemination have come from transposon insertion mutagenesis studies.

162 a catarrhalis ETSU-9 was subjected to random transposon insertion mutagenesis to identify genes encod

163 In this work, a transposon insertion mutant (cpsA::Tn) of Mycobacterium

164 , we report the isolation of a P. gingivalis transposon insertion mutant altered in biofilm developme

165 tely annotate the extremely large progenitor transposon insertion mutant collections needed to achiev

166 A transposon insertion mutant has been identified in a Des

167 A chlamydial transposon insertion mutant in the Cdu1-encoding gene ex

168 In previous work, we identified a transposon insertion mutant in the FTT0107c locus that w

169 Exposing a saturating transposon insertion mutant library of S Typhimurium to

170 Here, we describe the creation of a transposon insertion mutant library of the HSV-1 genome.

171 screening method to identify mutants from a transposon insertion mutant library which exhibited dist

172 of ZmMADS69 causes early flowering, while a transposon insertion mutant of ZmMADS69 exhibits delayed

173 The method, called transformation transposon insertion mutant sequencing (TFNseq), employs

174 his limitation, we developed a procedure for transposon insertion mutant sequencing that includes ess

175 measure the exometabolome of 86 single-gene transposon insertion mutant strains (mutants from centra

176 tularensis subsp. tularensis strain Schu S4 transposon insertion mutant with a mutation in a predict

177 study, we describe a Drosophila melanogaster transposon insertion mutant with tolerance to Vibrio cho

178 ised of approximately 31,500 Y. pestis KIM6+ transposon insertion mutants (input pool) was subjected

179 Certain in-frame transposon insertion mutants did not interact with DNA a

180 by which this regulation occurs, we screened transposon insertion mutants for those that could migrat

181 ed the relative fitness of 41,000 Salmonella transposon insertion mutants growing in mouse models of

182 prehensive library of 2,998 sequence-defined transposon insertion mutants in Francisella novicida str

183 Screening of approximately 3,000 transposon insertion mutants in the crystal violet-based

184 arrayed library of over 13,000 K. pneumoniae transposon insertion mutants in the lungs of wild-type (

185 A total of 4,330 transposon insertion mutants of an invasive G1 Nal(r) st

186 the GlpT homologs in F. novicida and tested transposon insertion mutants of glpT.

187 e cytotoxicity and biofilm formation from 93 transposon insertion mutants previously reported with in

188 a common mechanism exists, we have analyzed transposon insertion mutants resulting in independent lo

189 Two of the other three M. catarrhalis ETSU-9 transposon insertion mutants that had greatly reduced ab

190 genetic screen to identify L. monocytogenes transposon insertion mutants that induced altered levels

191 We screened E. coli transposon insertion mutants to look for proteins that m

192 The parental library of PA14 transposon insertion mutants was generated by using MAR2

193 Enterococcus faecalis transposon insertion mutants were screened for attenuate

194 ng a colony immunoblot screen, we identified transposon insertion mutants which were deficient for ty

195 tors involved in these processes, DC3000 Tn5 transposon insertion mutants with reduced virulence on A

196 Examining a library of transposon insertion mutants, we identified the LysR-typ

197 i was identified from a collection of random transposon insertion mutants.

198 We found that transposon-insertion mutants for 46% of the essential ge

199 A transposon insertion mutation in pgant3 or RNA interfere

200 stress defense, we isolated a mutant with a transposon insertion mutation of sitA, which encodes the

201 Transposon insertion mutations in Gemin3 are larval leth

202 ive mutants led to the identification of 174 transposon insertion mutations that mapped to 13 individ

203 To identify genes essential for A motility, transposon insertion mutations with defective A motility

204 e functions from the phenotypes arising from transposon insertion mutations.

205 Finally, we show novel transposon insertions negatively correlate with Piwi-int

206 In a second mutant, the transposon insertion occurred in mrpB, which is part of

207 ased in a transposon mutant (NBC-28G9) where transposon insertion occurred in the 5' end of gidA gene

208 e show that we can determine the effect of a transposon insertion on its target gene(s) and prioritiz

209 aDIS experiments that predicts the impact of transposon insertions on nearby genes.

210 Gene inactivation by transposon insertion or allelic exchange is a powerful a

211 (gTME), and gene-disruption methods such as transposon insertion or site-specific homologous recombi

212 h on the basis of the assumption that recent transposon insertions or excisions create singleton or l

213 t model of endocarditis to test strains with transposon insertions or in-frame deletions in biofilm-a

214 ction was used to explain observed miniHimar transposon insertion patterns, and gene essentiality cal

215 psis containing T-DNA insertion (pbs3-2) and transposon insertion (pbs3-3) mutations in At5g13320 con

216 To identify transposon insertion points, a unique primer directed ou

217 We perform transposon insertion profiling by microarray (TIP-chip)

218 insertions selectively in the human genome, transposon insertion profiling by next-generation sequen

219 Large-scale transposon insertion projects provide excellent material

220 tatistically significant number of recurrent transposon insertions, respectively.

221 n of this mutation by genetic removal of the transposon insertion restored MAb 2-1-L8 binding.

222 Here, we analyzed transposon-insertion rice lines disrupted in OsHKT2;1.

223 A transposon insertion screen implicated the yejH gene in

224 Using a genome-wide transposon insertion screen, we identified an operon req

225 Here, we tested whether transposon insertion sequencing (INSeq) could be used to

226 oson-mediated mutagenesis in a method termed transposon insertion sequencing (TIS).

227 ADP1 single-gene deletion strains and a new transposon insertion sequencing (Tn-Seq) dataset that we

228 Transposon insertion sequencing (Tn-Seq) is a microbial

229 Transposon insertion sequencing (Tn-seq) is an emerging

230 We used transposon insertion sequencing (Tn-Seq) to define essen

231 Transposon insertion sequencing is a high-throughput tec

232 Here, a transposon insertion sequencing technology called INSeq

233 Here we use transposon insertion sequencing to identify A. baumannii

234 ance of tRNAs in the absence of thiI Through transposon insertion sequencing, we identified additiona

235 en 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which com

236 s for V. cholerae survival, and by combining transposon-insertion sequencing and transcriptomic data

237 We have refined analysis of transposon-insertion sequencing data by normalizing for

238 re unappreciated information inherent in all transposon-insertion sequencing data sets.

239 nhance extraction of biological meaning from transposon-insertion sequencing genomic data.

240 In a transposon-insertion sequencing screen to identify mutan

241 We used transposon-insertion sequencing to screen for genes that

242 tagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous an

243 Here, we used a transposon insertion site (TIS) sequencing-based strateg

244 After determining the transposon insertion site for each mutant, unique revers

245 Using PCR, we identified a transposon insertion site in the first intron of Nmnat2

246 Jak2 was identified as a common transposon insertion site in TLS-ERG-induced disease, st

247 The transposon insertion site of this mutant strain was with

248 We have designed a transposon insertion site profiling chip (TIP-chip), a m

249 tion sequencing allows affordable ultra-deep transposon insertion site recovery in high-throughput fo

250 -mediated tethering can effectively redirect transposon insertion site selection in human cells, but

251 also describe a method for semiquantitative transposon insertion site sequencing (QiSeq).

252 We used transposon insertion site sequencing (Tn-seq) to compreh

253 Here, we carried out a high-throughput, transposon insertion site sequencing analysis (TnSeq) to

254 The transposon insertion site was identified for 29 of the 1

255 Transposon insertion-site sequencing was used to analyze

256 quence reads mapped to millions of potential transposon insertion sites and a large portion of insert

257 In the analysis of these screens, transposon insertion sites are typically identified by t

258 To determine whether genes located at transposon insertion sites directly caused medulloblasto

259 g (SBCapSeq), that facilitates sequencing of transposon insertion sites from single tumor cells in a

260 Sequence analysis of transposon insertion sites from tumors and immortalized

261 Isolation of the transposon insertion sites identified genes known to be

262 Analysis of transposon insertion sites identified novel candidate ge

263 After sequencing transposon insertion sites in 9,250 random mutants, we a

264 Characterization of transposon insertion sites in the SB-induced tumors iden

265 ficient methods were established to identify transposon insertion sites in these lesions, and analysi

266 n pathways were conspicuous among the common transposon insertion sites in TLS-ERG-driven leukemia, s

267 Common transposon insertion sites occur among haplotypes from d

268 alyze approximately 2 x 10(5) unique de novo transposon insertion sites of the transposon Hermes in t

269 ion of direct, high-throughput sequencing of transposon insertion sites revealed fitness phenotypes o

270 induced astrocytoma tissue was extracted and transposon insertion sites were identified.

271 ost of additional sequencing to characterize transposon insertion sites.

272 sensitive and robust system for identifying transposon insertion sites.

273 ive molecular framework for the interplay of transposon insertion, SNP/indel mutation, and epigenetic

274 We show that genetic complementation of the transposon insertion strain partially restored the trans

275 ion was attributed to a natural CACTA family transposon insertion that inactivates Bx10c in maize lin

276 divergence between maize and teosinte, and a transposon insertion that inactivates Bx12 was under str

277 Three mucoid mutants were identified with transposon insertions that caused 1) an overexpression o

278 also close to the locations of several gypsy transposon insertions that disrupt Ubx expression, leadi

279 Finally, we discovered a large set of transposon insertions that trigger premature initiation

280 tion to characterize genomic regions lacking transposon insertions, this method is capable of identif

281 oughput DNA sequencing, screened genome-wide transposon insertion (Tn-seq) and transcriptome (RNA-seq

282 struct appropriate statistical tests for the transposon insertion tolerance of normal genes of intere

283 Transposon insertions typically resulted in altered expr

284 Transposon insertions upstream of csgC and lrhA or withi

285 A library of over 41,000 unique transposon insertions was analyzed before and after colo

286 P-mediated recombination between FRT-bearing transposon insertions was used to generate deletions, be

287 s of D. melanogaster, carrying single P[GT1] transposon insertions, we found 267 lines that showed si

288 a single transgenic line harboring five Tnt1 transposon insertions, we generated a near-saturated ins

289 llisepticum genome, the precise locations of transposon insertions were discerned.

290 Recurrent and mutually exclusive transposon insertions were identified in Myh9, Ppp1r12a,

291 nsertions (0.86%) were biofilm negative, and transposon insertions were located in 51 distinct genes/

292 Single-copy transposon insertions were transposed into the rat genom

293 in exons (8%) may deter insertion of Mutator transposon insertion, while CHG methylation at splice ac

294 A new collection of Minos transposon insertions will enhance the range and flexibi

295 One of these mutants possessed a transposon insertion within a gene, designated mrkI, enc

296 psis line containing a knock-out dissociator transposon insertion within the single copy AtPLMT gene

297 Transposon insertions within genes for sulfate assimilat

298 ered a set of genetic suppressors harbouring transposon insertions within genes of a locus encoding A

299 The maternal disruption of imprinting by transposon insertions within the Mez1 promoter suggests

300 Three novel Mez1 alleles containing Mutator transposon insertions within the promoter were identifie