ライフサイエンスコーパス: transposon mutagenesis

1 avily mutagenized individuals (site-selected transposon mutagenesis).

2 mic features based on the in vitro effect of transposon mutagenesis.

3 tor of Y. pestis, we performed mariner-based transposon mutagenesis.

4 a forward genetic screen using gene-breaking transposon mutagenesis.

5 cid UL25 open reading frame was disrupted by transposon mutagenesis.

6 rtion mutants were created for both genes by transposon mutagenesis.

7 ted from a serotype C strain 26404 by random transposon mutagenesis.

8 ream of the pilin gene pilE was targeted for transposon mutagenesis.

9 ity of the genome design in C. crescentus by transposon mutagenesis.

10 le to form biofilms in vitro and amenable to transposon mutagenesis.

11 R-A specific deletion mutants isolated using transposon mutagenesis.

12 ial protein that could not be uncovered with transposon mutagenesis.

13 tant libraries can be readily constructed by transposon mutagenesis.

14 he strategy of differential signature-tagged transposon mutagenesis.

15 rus AD169 genome by random and site-directed transposon mutagenesis.

16 n ftfL homolog identified previously through transposon mutagenesis.

17 useful for reverse genetics than for forward transposon mutagenesis.

18 entation system in Escherichia coli based on transposon mutagenesis.

19 s) were identified, and each was analyzed by transposon mutagenesis.

20 iviparous 14 (vp14) was recently obtained by transposon mutagenesis.

21 ants of M. xanthus were isolated through Tn5 transposon mutagenesis.

22 sion (CCR) of the hut operon was isolated by transposon mutagenesis.

23 y osmotica were sought by a further round of transposon mutagenesis.

24 ant, viviparous14 (vp14), were identified by transposon mutagenesis.

25 ctive for macrophage killing was isolated by transposon mutagenesis.

26 re derived from wild-type strain AA100 after transposon mutagenesis.

27 cobacterium smegmatis was isolated following transposon mutagenesis.

28 F gene was identified by random miniTn10-tet transposon mutagenesis.

29 pacX was cloned by impala transposon mutagenesis.

30 In global transposon mutagenesis, 173 insertion mutants out of 15,

31 Previously, we had isolated by transposon mutagenesis a Legionella pneumophila mutant t

32 Using transposon mutagenesis, a gene, designated hmpF, was ide

33 Transposon mutagenesis, allelic replacement, and electro

34 After mini-mariner transposon mutagenesis, an SDS-resistant suppressor muta

35 Transposon mutagenesis and a visual screen were used to

36 The use of a combination of transposon mutagenesis and arginine hydroxamate selectio

37 gene encoding this protein was identified by transposon mutagenesis and characterized.

38 an macrophages was generated by Tn903dIIlacZ transposon mutagenesis and classified into DNA hybridiza

39 Transposon mutagenesis and comparative sequence analysis

40 ipid A portion of the LOS, was identified by transposon mutagenesis and construction of an isogenic k

41 e we report a high-throughput approach using transposon mutagenesis and deep sequencing (Tn-seq) to i

42 ve whole-genome analysis of gene function by transposon mutagenesis and deep sequencing methodology h

43 Transposon mutagenesis and genetic studies revealed that

44 ed a deletion allele of ERG by site-selected transposon mutagenesis and have shown that seeds contain

45 motes commensalism, we used signature-tagged transposon mutagenesis and identified 29 mutants represe

46 We used genome-wide transposon mutagenesis and insertion-site sequencing, RN

47 ction" technology that combines high-density transposon mutagenesis and massively parallel sequencing

48 construction, screening approaches combining transposon mutagenesis and microarray technology, and th

49 operon deletion mutant was subjected to Tn5 transposon mutagenesis and new mutants selected using a

50 tifying individually deletable regions using transposon mutagenesis and progressively clustering dele

51 a forward-genetic procedure involving random transposon mutagenesis and rapid phenotypic screening, w

52 We performed a transposon mutagenesis and screened for mutants with alt

53 Transposon mutagenesis and screening for increased beta-

54 n, we subjected a dnaA(cos) mutant strain to transposon mutagenesis and selected mutant derivatives t

55 ficiency was bacterial conjugation, enabling transposon mutagenesis and targeted knockouts using suic

56 Using near-saturation transposon mutagenesis and testing of transposon-tagged

57 We applied hypersaturated transposon mutagenesis and transposon-directed insertion

58 Subcloning, transposon mutagenesis, and DNA sequence analysis reveal

59 nsposon insertions, stringent suppression of transposon mutagenesis, and few polar effects.

60 nt alleles of the DIF1 gene were isolated by transposon mutagenesis, and the mutants show complete ma

61 The irrE locus of R1 was also inactivated by transposon mutagenesis, and this strain was sensitive to

62 of-principle for the utility of the low-copy transposon mutagenesis approach for identifying cancer-d

63 Using a Tn3-based transposon mutagenesis approach, we have generated a poo

64 Using a transposon mutagenesis approach, we have identified a mu

65 In this study, using a powerful transposon mutagenesis approach, we have identified in S

66 Finally, using a transposon mutagenesis approach, we identified a new pos

67 as previously generated by using a Tn3-based transposon mutagenesis approach.

68 alovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach.

69 es can be identified on a genome-scale using transposon mutagenesis approaches; however, variability

70 lr4311, and all4388, identified initially by transposon mutagenesis, are such genes by complementing

71 The ymoBA locus was identified by transposon mutagenesis as a repressor of inv expression

72 Whole genome genetic fitness analysis using transposon mutagenesis combined with next-generation hig

73 One locus identified by transposon mutagenesis conferred protection against heme

74 charomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequen

75 Transposon mutagenesis, coupled with a novel reporter sy

76 simple and rapid method to analyse multiple transposon mutagenesis data sets allowing this technolog

77 e of molecular biology combined with limited transposon mutagenesis data, failed to produce a viable

78 d be identified in the same parent strain by transposon mutagenesis, despite extensive searches.

79 ors to drug resistance and demonstrated that transposon mutagenesis drives the development of 5-fluor

80 A pilot-scale transposon mutagenesis experiment using a modified auton

81 model agrees well with genome-scale in vivo transposon mutagenesis experiments, showing a Matthews c

82 ompared with data obtained from recent dense-transposon mutagenesis experiments.

83 o screen isogenic mutants generated by Tn916 transposon mutagenesis for defective biofilm formation.

84 ional mutant mice by whole-exome sequencing, transposon mutagenesis forward genetic screening, and tr

85 Transposon mutagenesis has thus provided a better unders

86 Transposon mutagenesis identified a LysR-like regulator,

87 T-POP transposon mutagenesis identified marT as a positive reg

88 Random transposon mutagenesis identified null mutations and ove

89 Transposon mutagenesis identified several gene products

90 Transposon mutagenesis identified two monooxygenases, Ab

91 Transposon mutagenesis implicated lipopolysaccharide (LP

92 Transposon mutagenesis in bacteria generally requires ef

93 ghly transcribed operons were insulated from transposon mutagenesis in both organisms.

94 Here, using random transposon mutagenesis in conjunction with in vitro mode

95 al galactose-sensitive adhesin, a system for transposon mutagenesis in fusobacteria was created.

96 scribe an efficient system for site-selected transposon mutagenesis in maize.

97 Using transposon mutagenesis in mice many laboratories have co

98 odules to rapidly generate tagged mutants by transposon mutagenesis in the metal-reducing bacterium S

99 ates, and is therefore a valuable vector for transposon mutagenesis in vertebrate models and for huma

100 a number of useful derivatives suitable for transposon mutagenesis in vivo or in vitro.

101 Transposon mutagenesis, in combination with parallel seq

102 Transposon mutagenesis indicated that the two complement

103 A second drl2 allele, produced by transposon mutagenesis, interacted synergistically with

104 mbination of high-throughput sequencing with transposon mutagenesis is a powerful approach for the id

105 High-copy transposon mutagenesis is an effective tool for creating

106 Mitomycin C-sensitive clones from a transposon mutagenesis library were screened.

107 Using transposon mutagenesis, lrs mutants were found in 11 dif

108 ejuni have been limited due to the lack of a transposon mutagenesis method.

109 inally, we directly compared T-DNA and Ac/Ds transposon mutagenesis methods in Arabidopsis on a genom

110 Improved transposon mutagenesis methods revealed a class of quasi

111 2), we have employed high-throughput shuttle transposon mutagenesis of a cosmid library prepared in E

112 Transposon mutagenesis of a Deltahha esp::lac strain exp

113 We present here a method for in vivo transposon mutagenesis of a methanogenic archaeon, Metha

114 Transposon mutagenesis of a strain carrying a deletion s

115 ate the genetic controls governing swarming, transposon mutagenesis of a TR (DeltaopaR1) strain was p

116 We performed transposon mutagenesis of a two-color fluorescent report

117 Transposon mutagenesis of A. oris yielded a mutant defec

118 Transposon mutagenesis of Agrobacterium tumefaciens iden

119 Transposon mutagenesis of an encapsulated, virulent stra

120 e high-throughput process relies on in vitro transposon mutagenesis of an ordered cosmid library; mut

121 In this study, transposon mutagenesis of an unencapsulated strain yield

122 Transposon mutagenesis of Bordetella pertussis was used

123 nstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.

124 By conducting transposon mutagenesis of exoK mutants of R. meliloti an

125 Through transposon mutagenesis of F. tularensis subsp. holarctic

126 e results of this study indicate that random transposon mutagenesis of infectious B. burgdorferi is f

127 We combined high-coverage transposon mutagenesis of influenza virus with a rapid h

128 Transposon mutagenesis of L. enzymogenes revealed that t

129 ata demonstrate the utility of mariner-based transposon mutagenesis of M. marinum and that M. marinum

130 Transposon mutagenesis of Mycobacterium tuberculosis has

131 rol of AlgB which affect algD transcription, transposon mutagenesis of nonmucoid algB derivatives of

132 Furthermore, transposon mutagenesis of oxa-23 or interactors of Oxa-2

133 ine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1.

134 Here, we have used random transposon mutagenesis of R. rickettsii to generate a sm

135 Signature-tagged transposon mutagenesis of Salmonella with differential r

136 Transposon mutagenesis of SBW25DeltafleQ (SBW25Q) produc

137 volved in swarming, we carried out extensive transposon mutagenesis of serovar Typhimurium, screening

138 In this study we have undertaken transposon mutagenesis of SH1000 to identify components

139 Transposon mutagenesis of strain AW1-PC (phcA1) generate

140 Transposon mutagenesis of Synechococcus elongatus PCC 79

141 for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4.

142 . marinum model, we have developed efficient transposon mutagenesis of the organism by using a Drosop

143 Transposon mutagenesis of the rugose variant led to the

144 Transposon mutagenesis of this plasmid identified the po

145 tone carboxylase subunits were identified by transposon mutagenesis of X. autotrophicus and sequence

146 Transposon mutagenesis of X. autotrophicus revealed a re

147 Here, we report the isolation, by transposon mutagenesis, of tat mutant strains that have

148 , we describe a simple system for performing transposon mutagenesis on naturally transformable organi

149 tants were identified from a second round of transposon mutagenesis performed in an mtr efflux pump-d

150 ) mutants was generated by using a Tn3-based transposon mutagenesis procedure.

151 ess as a generic tool supporting genome-wide transposon mutagenesis programs, these data provide insi

152 Transposon mutagenesis provides a direct selection for m

153 Transposon mutagenesis provides a useful tool for ricket

154 ompletion of the trypanosome genome project, transposon mutagenesis provides an important addition to

155 Whole genome transposon mutagenesis revealed a 19-gene locus, involve

156 Subcloning and transposon mutagenesis revealed a 5.6-kb region, designa

157 Random transposon mutagenesis revealed that disruption of rsmA

158 A transposon mutagenesis screen for additional regulators

159 Our previous transposon mutagenesis screen identified 95 lung infecti

160 Finally, a magellan-4 transposon mutagenesis screen identified glcK, a putativ

161 driving HCC, we performed a near-saturating transposon mutagenesis screen in a mouse HBV model of HC

162 genetic forces driving TNBC, we performed a transposon mutagenesis screen in a phosphatase and tensi

163 er genes, we carried out a low-copy piggyBac transposon mutagenesis screen in mice.

164 s hypothesis by performing a Sleeping Beauty transposon mutagenesis screen in which common insertion

165 The Sleeping Beauty (SB) transposon mutagenesis screen is a powerful tool to faci

166 Following a global transposon mutagenesis screen of EAEC 042, the transcrip

167 Transposon mutagenesis screen of meningitis-causing E. c

168 A transposon mutagenesis screen revealed additional compon

169 reporter system to facilitate a large-scale transposon mutagenesis screen to identify genes required

170 We used it to perform a Sleeping Beauty transposon mutagenesis screen to identify genes that coo

171 We have conducted a transposon mutagenesis screen to identify mutants defici

172 We used a genome-wide transposon mutagenesis screen to identify where mutation

173 Here, we describe a genome-wide transposon mutagenesis screen to isolate recipient mutan

174 siological signals affecting this pathway, a transposon mutagenesis screen was carried out to find fa

175 A transposon mutagenesis screen was performed with the rep

176 In a transposon mutagenesis screen, three transfer-defective

177 Through an extensive transposon mutagenesis screen, we have identified severa

178 During a transposon mutagenesis screen, we identified a novel gen

179 pyogenes mutant, SalY, was identified from a transposon mutagenesis screen, with over 200-fold attenu

180 terium extorquens AM1 were identified from a transposon mutagenesis screen.

181 ncogene in nonmelanoma skin cancer through a transposon mutagenesis screen.

182 s required for transfer, we have performed a transposon mutagenesis screen; we report here that LpqM,

183 Signature-tag transposon mutagenesis screening of a 1520-member librar

184 In this study, using a transposon mutagenesis screening, we identified a cell s

185 ogression, we performed Sleeping Beauty (SB) transposon mutagenesis screens in mice carrying sensitiz

186 nally organized, we carried out whole-genome transposon mutagenesis screens in Mtb strains deleted fo

187 critical for Yop delivery, we initiated two transposon mutagenesis screens using the mariner transpo

188 Using two genome-wide transposon mutagenesis screens, we identified 69 GAS gen

189 We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomo

190 ains were derived by three distinct methods: transposon mutagenesis, serial passage in the presence o

191 Transposon mutagenesis showed that a P. aeruginosa mutan

192 ms in this system proved to be refractory to transposon mutagenesis, so an M. catarrhalis strain was

193 In this study, we use signature-tagged transposon mutagenesis (STM) to conduct a screen for ran

194 ative selection strategy of signature-tagged transposon mutagenesis (STM) to screen mutants for loss

195 In this study, signature-tagged transposon mutagenesis (STM) was used to identify genes

196 In this study, we used a transposon mutagenesis strategy based on a two-step Slee

197 Using a unique transposon mutagenesis strategy that mutagenizes NSCs in

198 A high-density transposon mutagenesis strategy was applied to the Haemo

199 ts were created in E. coli using an in vitro transposon mutagenesis strategy.

200 bserved for biotin biosynthesis genes during transposon mutagenesis studies in mouse infection models

201 Transposon mutagenesis studies strongly suggest that BAP

202 Previous transposon mutagenesis studies with this organism were b

203 sional gel electrophoresis, and whole-genome transposon mutagenesis studies.

204 This property enabled a transposon mutagenesis study and growth studies to confi

205 n regulation of this two-component system, a transposon mutagenesis study was designed to identify su

206 Transposon mutagenesis suggested three possible targets:

207 ut transient targeted mutagenesis and random transposon mutagenesis suggests that stable targeted kno

208 pping techniques, including fragmentation by transposon mutagenesis, synthetic peptides, phage-displa

209 Here we describe an in vitro transposon mutagenesis system based on the Staphylococcu

210 We have developed an IS903-based transposon mutagenesis system for diverse gram-negative

211 Here we report on the development of a transposon mutagenesis system for use with marine Synech

212 Using the candystripe1 transposon mutagenesis system in sorghum, we have isolat

213 The Sleeping Beauty (SB) transposon mutagenesis system is a powerful tool that fa

214 ard that end, we have developed an efficient transposon mutagenesis system that makes use of a Himar1

215 liding, we developed a plasmid-based mariner transposon mutagenesis system that works effectively in

216 A novel transposon mutagenesis system was established and used t

217 A random in vivo transposon mutagenesis system was recently developed for

218 The in vitro transposon mutagenesis technique was shown to be a power

219 saSBase(LsL), that allows the restriction of transposon mutagenesis to a specific tissue of interest.

220 nvestigate ZIKV genetic flexibility, we used transposon mutagenesis to add 15-nucleotide insertions t

221 ode hypothetical proteins were determined by transposon mutagenesis to be required for optimal photoa

222 ated from an ongoing study using large-scale transposon mutagenesis to characterize gene function in

223 A new study uses Sleeping Beauty transposon mutagenesis to drive osteosarcomagenesis in t

224 We have used transposon mutagenesis to generate a library of PLD1 all

225 f a supercoiling-sensitive promoter and used transposon mutagenesis to generate a wide range of mutan

226 The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing

227 In this study, we used random transposon mutagenesis to identify a 3-deoxy-D-manno-oct

228 We used Sleeping Beauty (SB) transposon mutagenesis to identify events that cooperate

229 Using Tn5-transposon mutagenesis to identify factors that upend le

230 We used transposon mutagenesis to identify genes responsible for

231 We used random transposon mutagenesis to identify genes that are requir

232 study, we examined the feasibility of using transposon mutagenesis to identify infectivity-related f

233 se findings demonstrate the utility of using transposon mutagenesis to identify low-frequency and coo

234 Herein, we used random transposon mutagenesis to identify LVS genes that affect

235 We used random transposon mutagenesis to identify new genes involved in

236 We used transposon mutagenesis to identify S. Enteritidis virule

237 on of ER-PM contact sites we used saturating transposon mutagenesis to identify synthetic lethal muta

238 Here, we employ genome-wide transposon mutagenesis to identify the genes that suppor

239 We report the use of transposon mutagenesis to identify two genes required fo

240 We used transposon mutagenesis to investigate whether other gene

241 we take advantage of haploinsufficiency and transposon mutagenesis to perform large-scale loss-of-fu

242 We performed an ISS1 transposon mutagenesis to screen for acid-sensitive muta

243 We developed a strategy for high-efficiency transposon mutagenesis to screen for genes in B. hensela

244 Using signature-tagged transposon mutagenesis to screen for novel virulence fac

245 targeted cre-dependent Sleeping Beauty (SB) transposon mutagenesis to the blood-forming system using

246 As a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier t

247 Using transposon mutagenesis, two mutant strains were identifi

248 nvestigations of hemA regulation, we applied transposon mutagenesis under aerobic conditions, followe

249 ve developed an efficient system of in vitro transposon mutagenesis using a mariner-based transposon

250 re, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon

251 bank consisting of 11,354 mutants by mariner transposon mutagenesis using Brucella melitensis rough m

252 Transposon mutagenesis was conducted to identify inserti

253 rganisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants

254 Random T-POP transposon mutagenesis was employed to screen for insert

255 SB transposon mutagenesis was performed in either a wild-ty

256 Saturating transposon mutagenesis was performed in wild type and a

257 s regulation of motility by type 1 fimbriae, transposon mutagenesis was performed on a phase-locked t

258 fy genes necessary for bile resistance, MudJ transposon mutagenesis was performed on a strain contain

259 tify factors that regulate toxin expression, transposon mutagenesis was performed under non-inducing

260 sign functions of the various ORFs, in vitro transposon mutagenesis was performed using the cosmid DN

261 A benR mutant isolated by random transposon mutagenesis was unable to grow on benzoate.

262 of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generate a series of

263 Random transposon mutagenesis was used to generate A. baumannii

264 role of O antigen in virulence and survival, transposon mutagenesis was used to generate B. abortus r

265 Transposon mutagenesis was used to generate mutants of S

266 Transposon mutagenesis was used to identify an open read

267 Transposon mutagenesis was used to identify loci which n

268 Transposon mutagenesis was used to identify new genes th

269 Global transposon mutagenesis was used to identify nonessential

270 Random MudJ transposon mutagenesis was used to identify PmrA-PmrB-re

271 Transposon mutagenesis was used to identify regulators o

272 Transposon mutagenesis was used to identify sprE, which

273 Saturating transposon mutagenesis was used to identify the loci res

274 In the present study, TnphoA transposon mutagenesis was used to identify the TNF-alph

275 Transposon mutagenesis was used to identify transcriptio

276 In this study, signature-tagged transposon mutagenesis was used to identify Yersinia ent

277 Transposon mutagenesis was used to locate genes involved

278 ophs of Mycobacterium bovis BCG, obtained by transposon mutagenesis, was used to select methionine au

279 Using transposon mutagenesis we identified genes that were ess

280 Using random transposon mutagenesis, we determined that the phenotype

281 -mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of th

282 Using transposon mutagenesis, we have identified several regul

283 Using transposon mutagenesis, we identified 14 novel dynamic a

284 Using transposon mutagenesis, we identified a stand-alone poly

285 Using transposon mutagenesis, we identified factors that contr

286 Using Sleeping Beauty (SB) transposon mutagenesis, we identified novel genetic driv

287 Using transposon mutagenesis, we identified surface cell antig

288 iniBAC induction in Mycobacterium marinum By transposon mutagenesis, we identified that the operon is

289 protein depletion system in combination with transposon mutagenesis, we identify mutants of P. aerugi

290 Using transposon mutagenesis, we isolated a noncytotoxic strai

291 Using global transposon mutagenesis, we isolated and characterized ge

292 ing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing t

293 sed whole-genome fitness analysis, combining transposon mutagenesis with high-throughput sequencing,

294 seq) is an emerging technology that combines transposon mutagenesis with next-generation sequencing t

295 Here we report the use of transposon mutagenesis with the deep-sea bacterium Photo

296 ions in M. xanthus is extraordinarily broad, transposon mutagenesis with this eukaryotic genetic elem

297 Using transposon mutagenesis with Tn3HoHo1 and a binary transf

298 ae cytadherence was previously identified by transposon mutagenesis with Tn4001.

299 to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used.

300 We hypothesized that transposon mutagenesis would complement targeted knockou