ライフサイエンスコーパス: trypan blue

1 bee stages were visualised by staining with trypan blue.

2 ane integrity, blocking propidium iodide and Trypan blue.

3 scence was quenched by a cell viability dye, trypan blue.

4 C-labeled OS before and after quenching with trypan blue.

5 briefly with physiological buffer containing Trypan blue.

6 of SLCLs was determined by cell counts with trypan blue.

7 the number of rod-shaped myocytes excluding trypan blue.

8 +/- 3% (n = 5) of the 28-day cells excluded trypan blue.

9 ure, as many as 90% of the cells accumulated trypan blue.

10 ells at 48 hours as assessed by exclusion of trypan blue.

11 142 to 2530 mOsm, with and without 0.5 mg/mL trypan blue.

12 emarkably toxic to RPE cells with or without trypan blue.

13 y in vivo staining of the choroidal layer by trypan blue.

14 paracellular tracer flux was evaluated with trypan blue.

15 sensitive than human RPE (ARPE-19) cells to trypan blue.

16 staining of the inner limiting membrane with trypan blue.

17 reated with four different concentrations of trypan blue (0.1%, 0.05%, 0.025%, and 0.0125%), in combi

18 Y5Y cells as examined by oxygen consumption, trypan blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-t

19 , compared with images obtained prior to MGd/trypan blue administration (11.6 +/- 4.2 [standard devia

20 udies, nonviable cell nuclei were labeled by trypan blue after cold preservation and brief warm reper

21 the penetration and distribution of the MGd/trypan blue agent into CBD walls.

22 Morphologically, staining with trypan blue and alizarin red S showed an apparently inta

23 Both trypan blue and black India ink stained and were retaine

24 -limbal injection technique was tested using trypan blue and black India ink.

25 Trypan Blue and Indocyanine Green appear to be most effe

26 Trypan blue and phenol red are used as examples of synth

27 IL-1beta/TNF death was necrosis by trypan blue and propidium iodide positivity, absence of

28 s assessed by permeability to the vital dyes trypan blue and propidium iodide.

29 factors for posterior capsule staining with trypan blue and techniques to avoid this complication.

30 eath within 24 h as assayed by the uptake of trypan blue and the release of lactate dehydrogenase.

31 l of serum-starved C2C12, HSM, and NCM (MTT, trypan blue) and prevented taxol-induced apoptosis (casp

32 development were reintervention, the use of trypan blue, and a long follow-up.

33 ransepithelial resistance, impermeability to trypan blue, and confocal microscopy confirmed functiona

34 Tissue was also incubated with trypan blue, and cross-species immunoreactivity was dete

35 ssessment of endothelial cell injury in H&E, trypan blue, and immunostained (TIE2/Tek) biopsy specime

36 Cell survival and apoptosis were tested by Trypan blue, annexin V, and cleaved caspase-3 assays.

37 ornea guttata (aOR, 1.35; P = 0.006); use of Trypan blue (aOR, 1.76; P < 0.001); mechanical pupil dil

38 ornea guttata (aOR, 1.35; P = 0.006); use of Trypan blue (aOR, 1.76; P < 0.001); mechanical pupil dil

39 The trypan blue assay and a microscope-based propidium iodid

40 Cell survival was determined by trypan blue assay and ATP levels were measured with ATP

41 xyuridine triphosphate nick-end labeling and trypan blue assays to quantitate apoptosis and necrosis.

42 alysed in methylthiazoltetrazolium (MTT) and trypan blue assays.

43 e majority of apoptotic eosinophils excluded trypan blue at 48 hours post CD69 ligation.

44 Trypan blue-based automated counters, typically recommen

45 ed by incubating ARPE-19 for 10 minutes with trypan blue, brilliant blue, bromophenol blue, fast gree

46 ion of colony formation or for cell death by trypan blue by up to 2 logs for paclitaxel and up to 1 l

47 Inadvertent posterior capsule staining with trypan blue can occur in eyes that appear structurally n

48 e and the small intestine as demonstrated by Trypan blue cholangiography, and a liver histological pi

49 Trypan blue colocalized to the cut edge of retinal flatm

50 Trypan blue distribution time, an indicator of the hepat

51 Trypan blue distribution time, which is used to index th

52 in the rinse effluent, serum transaminases, trypan blue distribution to index microcirculation, and

53 EC injury was evaluated by trypan blue, DNA fragmentation, membrane phosphatidylser

54 Secondary measures included the use of trypan blue during CCC and correlating errant CCC and su

55 Cell viability was determined with a trypan blue dye assay.

56 ty or cellular DNA synthesis as evaluated by trypan blue dye exclusion and [3H]-thymidine incorporati

57 Cell death was evaluated by trypan blue dye exclusion and a more efficient specific

58 Cell viability was measured by a trypan blue dye exclusion assay.

59 Cell survival was determined with the trypan blue dye exclusion test.

60 Trypan blue dye exclusion was used to assess cell viabil

61 Cell viability was assayed by trypan blue dye exclusion, and cleaved caspase-3 immunor

62 ion of the tetrazolium compound to formazan, trypan blue dye exclusion, and clonogenic assays.

63 ated from the rat retina were assessed using trypan blue dye exclusion, perforated-patch recordings,

64 ntaining retinal microvessels was assayed by trypan blue dye exclusion.

65 lution (gadopentetate dimeglumine mixed with trypan blue dye) in and around the prostate, thereby con

66 Toxicity was determined by trypan blue dye-exclusion and WST-1 mitochondrial dehydr

67 function, and DNA synthesis were measured by trypan blue dye-exclusion assay, mitochondrial dehydroge

68 Cell viability (CV) was determined by a trypan blue dye-exclusion assay.

69 Cell viability was determined by a trypan blue dye-exclusion assay.

70 living and dead cells as distinguished with trypan blue dye.

71 assessed by viable cell recovery (VCR) with trypan blue, ethidium bromide/acridine orange staining,

72 Trypan blue exclusion analysis suggested that the loss o

73 ious human tumor cell lines were assayed for trypan blue exclusion and ability to form colonies after

74 Viability was assessed by trypan blue exclusion and apoptosis by morphologic asses

75 enza virus-induced cell death as measured by trypan blue exclusion and caspase activity.

76 attenuation of cytotoxicity, as measured by trypan blue exclusion and colony formation after treatme

77 Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent

78 lular toxicity or apoptosis as determined by trypan blue exclusion and FACS analyses.

79 companied by assessment of cell viability by trypan blue exclusion and flow cytometry; fluid dynamic

80 e number of viable thymocytes as assessed by trypan blue exclusion and fluorescence-activated cell so

81 o reduce MTT which was followed by decreased Trypan blue exclusion and increased Lactate dehydrogenas

82 Necrosis was determined by trypan blue exclusion and lactate dehydrogenase release

83 ted to 90 min of SI and 2 h RO for necrosis (trypan blue exclusion and lactate dehydrogenase release)

84 and DNA laddering, or necrosis, assessed by trypan blue exclusion and lactate dehydrogenase release.

85 Necrosis was determined using trypan blue exclusion and LDH release assay and apoptosi

86 s exhibited no cytotoxicity, as evaluated by trypan blue exclusion and mitochondrial function assays.

87 Trypan blue exclusion and MTT assays evaluated TSA cytot

88 Using trypan blue exclusion and MTT assays, there was no evide

89 Besides, Trypan Blue Exclusion and MTT methods were used to measu

90 s following toxin treatment, as evidenced by trypan blue exclusion and neutral red uptake.

91 th than non-ischemic controls as assessed by Trypan Blue exclusion and propidium iodide (PI) uptake o

92 The trypan blue exclusion and TUNEL assays were used to stud

93 lant were evaluated histologically utilizing trypan blue exclusion as an indicator of cell viability.

94 disruption was measured quantitatively by a trypan blue exclusion assay and by release of (3)H from

95 ination induced up to 40-50% cell death by a trypan blue exclusion assay in a dose-dependent manner u

96 Cell death was evaluated by trypan blue exclusion assay in corneal fibroblasts treat

97 d PDLF and GF by DNA fragmentation assay and trypan blue exclusion assay, either in the presence or a

98 sensitive fluorescent dye, Fluo-3, AM, and a trypan blue exclusion assay, we evaluated the ability of

99 Cell viability was evaluated by trypan blue exclusion assay.

100 344 lymphoid cells in a complement-dependent trypan blue exclusion assay.

101 ble cell number was also evaluated using the Trypan blue exclusion assay.

102 s and cell proliferation on the basis of the trypan blue exclusion assay/methylthiazolyldiphenyl-tetr

103 and apoptosis of hFOB cells were assessed by trypan blue exclusion assays and 4',6-diamidino-2-phenyl

104 Cytotoxicity was examined qualitatively by trypan blue exclusion assays and quantitatively by chrom

105 Cytotoxic activity was assessed by trypan blue exclusion assays with two cytotoxic strains

106 e-mediated dUTP-biotin nick end labeling and trypan blue exclusion assays, as well as lactate dehydro

107 n survival assays, and cytotoxic activity by Trypan blue exclusion assays.

108 48.8% +/- 10.3% of the cells being viable by trypan blue exclusion at 600 and 300 microg/ml, respecti

109 ehydrogenase into the medium and decrease in trypan blue exclusion by cells in the monolayer.

110 Cell viability was assessed via Trypan Blue exclusion cell counts, and neuronal damage w

111 No change in RPE morphology or trypan blue exclusion compared with controls was observe

112 Viability measurements by trypan blue exclusion confirmed that the injury was larg

113 Trypan blue exclusion deemed that these doses were not c

114 Viability measured by trypan blue exclusion indicated that cells were intact u

115 l morphology were also assessed by using the trypan blue exclusion method and scanning electron micro

116 The trypan blue exclusion method was used to test RPE cell v

117 Human RECs exposed to TA were assayed for trypan blue exclusion or activated caspase-3.

118 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the be

119 Our data from microscopy studies, trypan blue exclusion staining, and apoptotic DNA ladder

120 Morphologic and trypan blue exclusion techniques were used for toxicity

121 r combination was evaluated in cytotoxicity (Trypan Blue exclusion) and cell migration (Boyden Chambe

122 Cytotoxic effects (trypan blue exclusion) are first noted at 50 microM EB i

123 so-PGF2alpha had no effect on the viability (Trypan Blue exclusion) of U937 monocytes or HMEC.

124 No cellular toxicity (trypan blue exclusion) was observed at 20 muM, and photo

125 ell number (by cell counting), viability (by trypan blue exclusion), and cellular metabolic activity

126 , Bcl-2 inhibited loss of cell viability (by trypan blue exclusion), the appearance of morphologicall

127 iated EC apoptosis (DNA content analysis and trypan blue exclusion).

128 Trypan blue exclusion, [(3)H]thymidine incorporation, an

129 ieved and assessed, were < or = 2% intact by trypan blue exclusion, and none of the intact cells were

130 Cell growth was measured by trypan blue exclusion, and the MTT assay and apoptosis w

131 XTT conversion, (3)H-leucine incorporation, trypan blue exclusion, and the presence of DNA laddering

132 poptosis, and proliferation were measured by trypan blue exclusion, annexin-V/7-Aminoactinomycin D st

133 10 of 11 cell lines evaluated as well as in trypan blue exclusion, cellular ATP or caspase 3/7, 8 an

134 HA-BAD-expressing clones was demonstrated by trypan blue exclusion, clonogenic cell assay, and flow c

135 Apoptosis was monitored by trypan blue exclusion, colorimetric cell assay, CPP32 ac

136 Apoptosis was quantified by trypan blue exclusion, DNA fragmentation, and caspase 3

137 Apoptosis was examined using trypan blue exclusion, laddering of DNA, CPP32 assay, an

138 lity as measured by total ATP concentration, trypan blue exclusion, or 3-(4,5-dimethylthiazol-2-yl)-2

139 vival by bromodeoxyuridine incorporation and trypan blue exclusion, respectively, upon stimulation of

140 orphology, release of lactate dehydrogenase, trypan blue exclusion, the 3-(4,5-dimethylthiazol-2-yl)-

141 Based on trypan blue exclusion, the cells obtained from clinical

142 MPO release was noncytotoxic as assessed by trypan blue exclusion.

143 fected in parallel were intact, as judged by trypan blue exclusion.

144 cell growth and survival as determined using trypan blue exclusion.

145 age against the conventional assays, such as Trypan blue exclusion.

146 stances from the impact site was assessed by trypan blue exclusion.

147 Cell viability was analyzed with trypan blue exclusion.

148 briefly with physiological buffer containing trypan blue for determination of sinusoidal endothelial

149 The use of trypan blue increased from 55.3% in the baseline cohort

150 ise ratios of CBD walls before and after MGd/trypan blue infusions were compared in the two groups by

151 s of a transcholecytic access to deliver MGd/trypan blue into CBD walls of six living pigs.

152 eriments demonstrated the penetration of MGd/trypan blue into the CBD walls.

153 Trypan blue is US Food and Drug Administration-approved

154 Cell viability was assessed by Trypan Blue, lactate dehydrogenase (LDH) release and MTT

155 ce, and cytotoxicity was monitored using the trypan blue life/death assay directly on the surface.

156 teries of seven living pigs, and the MGd and trypan blue mixture was infused into arterial walls with

157 In addition, an MGd/trypan blue mixture was locally infused into CBD walls o

158 ere also associated with marked increases in trypan blue nuclear staining of A549 monolayers.

159 d by lactate dehydrogenase (LDH) release and trypan blue nuclear staining.

160 Addition of contrast agents (trypan blue or brilliant blue R) improve the signal-to-n

161 levels of AMF colonization is staining with trypan blue or ink, which is scored using the time-consu

162 preserved cellular integrity as measured by trypan blue or propidium iodide exclusion and [ATP].

163 gene-vector media by mixing Magnevist with a trypan-blue or a lentiviral vector carrying a green fluo

164 [95% CI 1.2-13.4], P = 0.02), and the use of trypan blue (OR = 4.1 [95% CI 1.3-13.1], P = .02) as pre

165 cells (14.8% at 24 h and 34.4% at 48 h) and trypan blue-positive apoptotic cells (8.4% at 24 h and 2

166 the level of DNA fragmentation and decreased trypan blue-positive cells in PDLF compared to GF during

167 reduced necrosis as indicated by decrease in trypan blue-positive myocytes and leakage of lactate deh

168 nusoidal endothelial cell injury by counting trypan blue-positive nuclei in histological sections.

169 time, position, and mode of fusion, aided by trypan blue quenching of Qdot fluorescence, revealed tha

170 orter axial length, longer follow-up, use of trypan blue, reintervention, and bilateral surgery were

171 vivo experiment confirmed the uptake of MGd/trypan blue, showing an increased contrast-to-noise rati

172 r, and mitochondrial bioenergetics utilizing trypan blue, Southern blotting, and extracellular flux a

173 o observed reduced cell death, visualized by trypan blue stain and reduced electrolyte leakage, in th

174 ce under basal conditions and an increase in trypan blue stained cells.

175 ecrease in cell viability and an increase in trypan blue-stained cells, cell apoptosis, and cleaved c

176 None of the samples showed trypan blue-stained cells.

177 ificantly greater number of hepatocytes were trypan blue-stained in fatty livers (32 vs. 0.6%), accom

178 ified by specular microscopy and counting of trypan-blue-stained cells.

179 ttenuated neuronal cell death as assessed by trypan blue staining 5-6 h after the exposures.

180 idative stress; cell death was determined by trypan blue staining and deoxyribonucleic acid (DNA) lad

181 Cytotoxicity was assessed by observation of trypan blue staining and measurement of lactate dehydrog

182 Trypan blue staining confirmed cell death and demonstrat

183 Trypan blue staining demonstrated the viability of endot

184 l with the population-averaged viability and trypan blue staining experiments.

185 Trypan blue staining indicated that 72 to 100% of cells

186 l viability assays such as the commonly used trypan blue staining method to coral cells is not straig

187 ng after storage/reperfusion, as assessed by Trypan blue staining of nonparenchymal cells.

188 Trypan blue staining revealed that PMNs cocultured with

189 ametazime-labeled leukocytes examined by the trypan blue staining technique at 6-hr postradiolabeling

190 sion (gentamicin survival) and cytotoxicity (trypan blue staining) of PA103 mutants deficient in spec

191 xyuridine incorporation), cell viability (by trypan blue staining), and apoptosis (by annexin V stain

192 of MLO-Y4 osteocyte-like cells, assessed by trypan blue staining, caspase-3 cleavage, and TUNEL assa

193 Cell viability was assessed by trypan blue staining, cell counting, and phase-contrast

194 hmmeter), and cytotoxicity was determined by trypan blue staining.

195 l)-2,5-diphenyltetrazolium bromide assay and Trypan blue staining.

196 Cell viability was determined with trypan blue staining.

197 on of p44/42 MAP kinase and reduced both the trypan-blue staining (n = 13) and the release of lactate

198 Cell viability was determined by Trypan Blue (TB) exclusion; cell injury was determined b

199 a greater percentage of cells staining with trypan blue than when cells were transfected with either

200 rvest, proteolytic activity and the time for trypan blue to distribute homogeneously were elevated th

201 e membrane-impermeable fluorescence quencher trypan blue to selectively image internalized alpha-syn

202 e staining by allowing the posterior flow of trypan blue under the iris and through the zonules to th

203 nces of caspase-3 activity, cell detachment, trypan blue uptake and aberrant nuclei were all delayed

204 The increased trypan blue uptake and thiobarbituric acid reactive subs

205 Trypan blue uptake by the HL-60, but not the K562 cells,

206 The increased trypan blue uptake in 355 nm-irradiated cells in the pre

207 A 24-h exposure to 250-300 nM resulted in trypan blue uptake in 50% of A549 cells at 72 h and a 50

208 The SLC trypan blue uptake was increased but similar in both gro

209 Plasma membrane permeability assessed by trypan blue uptake was used to measure cell damage in mu

210 Hepatocyte and SLC trypan blue uptake were minimal and similar in both grou

211 hibiting less lactate dehydrogenase release, trypan blue uptake, and apoptotic cell death (terminal d

212 by inhibition of colony formation, increased trypan blue uptake, and development of apoptotic morphol

213 y included leakage of lactate dehydrogenase, trypan blue uptake, morphology, and formazan production.

214 rter proteins, GFP and luciferase, or induce trypan blue uptake, which indicated that expression of D

215 n, caspase activation, PARP degradation, and trypan blue uptake.

216 s assessed morphologically and quantified by trypan blue uptake.

217 ed after irradiation did not cause increased trypan blue uptake.

218 a significant decrease in hepatocyte and SLC trypan blue uptake.

219 Trypan blue was infused at completion of reperfusion to

220 x vivo evaluation, a 2-mL mixture of MGd and trypan blue was locally infused into coronary artery wal

221 ifference in the rate of errant CCCs whether trypan blue was used or not.

222 a prelude to necrotic cell death and the dye trypan blue was used to confirm that swelling can result

223 leting model of ischemia, cells permeable to trypan blue were counted as killed by 60 and 120 min of

224 ive cases of posterior capsule staining with trypan blue were identified from cataract surgeries perf

225 R28 cells exposed to 0.1% trypan blue with and without light showed a significant

226 ARPE-19 and R28 cells exposed to trypan blue with or without illumination did not show an

227 ARPE-19 cells exposed to trypan blue, with or without light, did not show any sig

228 essful intracoronary infiltration of MGd and trypan blue within coronary artery walls.