ライフサイエンスコーパス: tubulin

1 ither spontaneously or by oligomers of gamma-tubulin.

2 anging the microenvironment of the activated tubulin.

3 d the proportion of Galpha(s) complexed with tubulin.

4 sh of the compound with the T5-loop of alpha-tubulin.

5 ein, identified by mass spectrometry as beta-tubulin.

6 a polymerase or acts dependently with gamma-tubulin.

7 , is the most abundant modification of brain tubulin.

8 to the disordered C-terminal tails (CTTs) of tubulin.

9 despread microtubule nucleation factor gamma-Tubulin.

10 ne 5a targets the colchicine-binding site on tubulin.

11 skeleton proteins such as actin, myosin, and tubulin.

12 ule-associated protein-2 (MAP-2) and betaIII-tubulin.

13 of the tails results in a step of monomeric tubulin.

14 nonoscapine) binds to the colchicine site of tubulin.

15 uring mitosis through interacting with alpha-tubulin.

16 tion of both the alpha- and beta-subunits of tubulin.

17 tubulin mRNAs in response to excess soluble tubulin.

18 el and engages the amino terminus of nascent tubulins.

19 ial cell division protein FtsZ (a homolog of tubulin); 5) is competitive with paclitaxel for binding

20 Tubulin, a Rossmann GTPase, demonstrates the potential o

21 a binder against terminal tyrosine of alpha-tubulin, a unique PTM site.

22 Here we found that detyrosinated alpha-tubulin accumulates on correct, more stable, kinetochore

23 Galpha(s) to lipid rafts and that increased tubulin acetylation (due to HDAC6 inhibition) and antide

24 While tissue homogenate showed no changes in tubulin acetylation between control, depressed suicides,

25 lly, we found that kindlin-2 maintains alpha-tubulin acetylation by inhibiting the microtubule-associ

26 ependently, we found 35.99% and 16.11% alpha-tubulin acetylation for mouse spinal cord and brain homo

27 tudy reveals a direct link between decreased tubulin acetylation in human depression and the increase

28 This study examined tubulin acetylation in whole-tissue homogenate, plasma m

29 dynamics characterized by differential alpha-tubulin acetylation is a hallmark of cancer, neurodegene

30 Hence, accurate quantitation of alpha-tubulin acetylation is required in human disease and ani

31 in expression of the enzymes responsible for tubulin acetylation or deacetylation.

32 ibody-free proteomics assay to measure alpha-tubulin acetylation targeting protease AspN-generated pe

33 oteomics assay enables quantitation of alpha-tubulin acetylation, and is applicable across various fi

34 AC6), a cytosolic deacetylase that regulates tubulin acetylation, in CF mice restores growth and infl

35 We hypothesized that alpha-tubulin acetyltransferase (alphaTAT), which both stabili

36 hus, our study reveals an important role for tubulin acetyltransferase activity in presynaptic mainte

37 We reveal a high gamma-/alphabeta-tubulin affinity, which facilitates assembly of a MT fro

38 We find that microtubules polymerized from tubulin alone can treadmill, albeit with opposite direct

39 eptides from the C-terminal segment of human tubulin alpha1A/B.

40 a molecular ratio of about one zinc atom per tubulin-alphabeta dimer.

41 Post-translational modifications of tubulin also alter microtubule dynamics.

42 Reduction of gamma-tubulin also rescued dendrite regeneration in background

43 (myosin) and cytoskeletal proteins (desmin, tubulin) also underwent 4HNE adduction.

44 A recent in vitro study demonstrated that tubulin anchors Galpha(s) to lipid rafts and that increa

45 tubulin heterodimers, which exist in soluble tubulin and at sites of microtubule polymerization and d

46 A-mediated interaction of Merlin with alpha-tubulin and ezrin suggest a potential role for Merlin in

47 rations in levels of neurite markers betaIII tubulin and fibroblast growth factor 12, with differenti

48 In the past, studying the contribution of tubulin and microtubules to spindle assembly has been li

49 osis and cell cycle analysis, and effects on tubulin and microtubules).

50 evel of knockdown of two target genes, alpha-tubulin and mitochondrial RNA polymerase (mtpol), were s

51 ocesses in nucleation, one promoted by gamma-tubulin and one promoted by XMAP215.

52 microtubule stabilizers covalently bind beta-tubulin and overcome clinically relevant taxane resistan

53 podocytes showed dispersed acetylated alpha-tubulin and rare protrusions.

54 levels of neuronal cytoskeletal marker beta3-tubulin and synaptic marker postsynaptic density 95 prot

55 nown molecules, including the template gamma-tubulin and the polymerase XMAP215.

56 rker O4), neuronal markers (nestin and B-III-tubulin) and fibroblast-associated markers (CD90/Thy1 an

57 (nestin), (c) neuronal phenotype (BrdU/beta3-tubulin), and (d) neuronal maturity (NeuN) in the subven

58 tworks based on polymerized proteins: actin, tubulin, and driven by motor proteins, such as myosin, k

59 , in turn, inhibits the acetylation of alpha-tubulin, and promotes the dynamic assembly of microtubul

60 racellularly and CD44 colocalized with alpha-tubulin as a result of MSU exposure and ECD-shedding red

61 XMAP215 and gamma-tubulin promote alphabeta-tubulin assembly in an additive, not synergistic, manner

62 that reduces the nucleation lag seen in bulk tubulin assembly.

63 Acetylation of alpha-tubulin at conserved lysine 40 (K40) amino acid residue

64 ledge of the amount of soluble and polymeric tubulin at mitotic centrosomes.

65 We report that [free tubulin] at steady state does not equal CC(NetAssembly),

66 ome or nascent tubulin interaction abolished tubulin autoregulation and showed chromosome segregation

67 vels after enough hours have passed to allow tubulin autoregulation to proceed.

68 DNA fingerprinting through tubulin-based polymorphism (TBP) and a new assay, TBP li

69 ase A2 receptor, protein kinase C zeta type, tubulin beta-4B class IVb, vimentin), only antibodies ag

70 Beta-tubulin (beta-Tub) was used as a loading control to esti

71 Mutations disrupting tubulin binding decrease microtubule density at the lead

72 ing edge of polarized cells, suggesting that tubulin binding may play a role in MEKK1 activity at the

73 h is free of this impurity, does not exhibit tubulin-binding activity.

74 ers encode tubulin proteins and a variety of tubulin-binding and -regulating proteins, but it is very

75 Ablation of tubulin-binding cofactors (TBCs) further sensitizes cell

76 We found that Tau's PRR is an independent tubulin-binding domain that has tubulin polymerization c

77 e or absence of a polymerization catalyst or tubulin-binding drugs.

78 We also show that MEKK1 mutations at the tubulin-binding interface of the TOG domain recur in pat

79 d that sequesters the kinesin motor domain's tubulin-binding surface, structurally distorting the mot

80 teractions of the alpha-tubulin CTT with the tubulin body contributes to the stiffness of the assembl

81 ly ordered cytoskeleton components actin and tubulin both in vivo and in vitro.

82 s competitive with paclitaxel for binding to tubulin but not with vinblastine, crocin, or colchicine;

83 Centrosomes concentrate soluble alpha/beta tubulin by about 10-fold over the cytoplasm, reaching pe

84 in spindles and functions with the substrate tubulin by an unknown mechanism.

85 show that inhibiting the maturation cycle of tubulin by using a point mutant in beta-tubulin confers

86 fined by the mean size of the protective GTP-tubulin cap.

87 lly add glutamates to internal glutamates in tubulin carboxy-terminal tails (branch initiation, throu

88 VASH1- and VASH2-SVBP complexes function as tubulin carboxypeptidases in cardiomyocytes, with a pred

89 VASH-SVBP complexes are active tubulin carboxypeptidases in cardiomyocytes.

90 d chimera versions of TPX2 suggest that TPX2-tubulin co-condensation enhances the efficiency of TPX2-

91 TPX2-tubulin co-condensation preferentially occurs on pre-exi

92 motors in a specific manner, widely known as tubulin code hypothesis.

93 tin code," similar to the "histone code" or "tubulin code," controlling functional shifts to these ce

94 rms and post-translational modifications, a "tubulin code," which is thought to support the diverse m

95 overing the mechanistic underpinnings of the tubulin code.

96 pindle MTOCs distribution, that is the gamma-tubulin complex receptor Spc72 and the protein Kar9, and

97 The Spc110 C terminus links the gamma-tubulin complex to the central plaque of the SPB by bind

98 in, Spc110, binds to and activates the gamma-tubulin complex via its N terminus, allowing nuclear mic

99 giving a combined total monomer and polymer tubulin concentration at centrosomes of up to 660 uM.

100 Tubulin concentration is autoregulated by feedback contr

101 ous assembly conditions, including different tubulin concentrations, the presence or absence of a pol

102 e of tubulin by using a point mutant in beta-tubulin confers hyperstable microtubules at low temperat

103 erences in intrinsic properties of different tubulins contribute to the control of microtubule mass a

104 a number of cytoskeletal proteins, including tubulin, cortactin, and the formin mDia2, regulates both

105 Our results show that the alpha-tubulin CTT does not protrude out from the microtubule s

106 suggests that the interactions of the alpha-tubulin CTT with the tubulin body contributes to the sti

107 results reveal an unappreciated step in the tubulin cycle.

108 lterations through its effector PAK1 and the tubulin cytoskeleton, thereby enhancing migration and in

109 C6) is a multidomain cytosolic enzyme having tubulin deacetylase activity that has been unequivocally

110 Immunostaining of beta-III tubulin demonstrated that corneal nerve fiber metrics we

111 ) methanone (abbreviated as QW-296), a novel tubulin destabilizing agent with little susceptible to t

112 and VASH1B, respectively) mRNA and elevated tubulin detyrosinase activity in cells.

113 identified a novel regulatory mechanism for tubulin detyrosinase, and illustrated that the VEZF1- an

114 shown to regulate angiogenesis by acting as tubulin detyrosinases.

115 ic error correction, and the extent of alpha-tubulin detyrosination allows centromeric MCAK to discri

116 the mitotic errors caused by excessive alpha-tubulin detyrosination independently of its global impac

117 activities to constitutively increase alpha-tubulin detyrosination near kinetochores compromised eff

118 totic error correction is regulated by alpha-tubulin detyrosination remains unknown.

119 ose activity in vitro is suppressed by alpha-tubulin detyrosination-a posttranslational modification

120 signaling and metabolic inputs that trigger tubulin differential expression, suggesting their novel,

121 y, conformation changes (compression) in the tubulin dimer following the hydrolysis of GTP have been

122 As a result, when the GDP state tubulin dimer is exposed at the growing MT end, these fa

123 the AAA motor exerts the forces that extract tubulin dimers and sever the microtubule.

124 s), cylindrical protein polymers composed of tubulin dimers are key components of the cytoskeleton.

125 gated the underlying interaction energy when tubulin dimers associate laterally by performing all-ato

126 ognition and efficient deacetylation of free tubulin dimers both in vitro and in vivo Overall, our re

127 MD potential energy in our BD simulations of tubulin dimers confirms that the lateral bond is weak on

128 by the conformation and nucleotide state of tubulin dimers within the microtubule lattice.

129 iciently with a cooperativity of 4 alphabeta-tubulin dimers.

130 ed by a "GTP cap" that consists of GTP-bound tubulin dimers.

131 uanosine triphosphate hydrolysis is found in tubulin (dis-)assembly.

132 taneously, thereby quantifying the alphabeta-tubulin dissociation constant (8.48 +/- 1.22 nM) and its

133 ter the removal of the compound; 3) binds to tubulin [dissociation constant (K(d)) 0.4 +/- 0.1 muM] a

134 -like kinase 1 signaling underlies the gamma-tubulin distribution defects observed with Gravin loss.

135 a brief historical overview of research into tubulin diversity and highlight recent progress toward u

136 ytoskeleton, understanding how cells utilize tubulin diversity is crucial to understanding cellular p

137 nsition where laterally associated alphabeta-tubulins drive gamma-TuRC into a closed conformation.

138 thoxy group of noscapine prevents binding to tubulin due to a steric clash of the compound with the T

139 ulation and asymmetric distribution of gamma-tubulin during mitosis.

140 pounds, including agents that interfere with tubulin dynamics and reduced cyst growth without affecti

141 Acetylation of tubulin enables microtubules emanating from this centre

142 ta-tubulin harboring Cys239, but not beta(3) tubulin encoding Ser239.

143 did not extend neurites or increase beta-III tubulin expression.

144 hollow tapered barrel structure composed of tubulin fibers.

145 ng the motor in cultured cells and measuring tubulin fluorescence levels after enough hours have pass

146 ion of discontinuous, short acetylated alpha-tubulin fragments, and the decrease of microtubule-rich

147 rization of differentially modified pools of tubulin from Tetrahymena thermophila.

148 We seek to investigate tubulins from different tissues and different organisms

149 be controlled by titrating the ratios of the tubulins from the two frog species.

150 Mutations in tubulin genes are associated with severe human brain mal

151 Essential effector proteins read the tubulin glutamylation pattern, and its misregulation cau

152 F phenotype, while spermadhesin-1, gelsolin, tubulins, glyceraldehyde-3-phosphate dehydrogenase, calm

153 ired growth of microtubules formed with beta-tubulin harboring Cys239, but not beta(3) tubulin encodi

154 Cell lines expressing mutant beta-tubulin have also been reported to be resistant to rigos

155 The alphabeta-tubulin heterodimer is the fundamental building block of

156 spindle's main building block, the alphabeta-tubulin heterodimer, has yet to be studied.

157 s and clarify the energetics and kinetics of tubulin heterodimerisation.

158 e their relationship from binding alpha-beta tubulin heterodimers to the larger proportions of microt

159 trates a clear preference for binding curved tubulin heterodimers, which exist in soluble tubulin and

160 The MEKK1 TOG domain binds to tubulin heterodimers-a canonical function of TOG domains

161 Bacterial tubulin homolog FtsZ self-assembles into dynamic protofi

162 FtsZ protein is a highly conserved bacterial tubulin homolog.

163 y coupled to the dynamic behavior of FtsZ, a tubulin homolog.

164 ization, modulates the acetylation status of tubulin, HSP90, TGF-beta, and peroxiredoxins.

165 berrant expression of the neural marker beta-tubulin III, and an overall reduction in numbers of cort

166 alter the distal border of centrosomal gamma-tubulins, illustrating a new role of sDAPs.

167 get engagement was assessed by confocal anti-tubulin immunofluorescence to quantify microtubule bundl

168 different from those revealed by anti-alpha-tubulin immunostaining, making these two neuronal marker

169 ated gene expression of NeuN, MAP-2, betaIII-tubulin in addition to growth-associated protein-43 (GAP

170 oton count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

171 We found that Arl4D colocalized with gamma-tubulin in centrosomes and the depletion of Arl4D result

172 co-localization at the nanoscale of zinc and tubulin in dendrites with a molecular ratio of about one

173 ing the spatial proximity of alpha- and beta-tubulin in microtubules using super-resolution detection

174 e reduces levels of the major TRiC substrate tubulin in P. falciparum parasites.

175 ASP repairs lattice damage by regulating GTP-tubulin incorporation into the break site.

176 hes CC(NetAssembly) asymptotically as [total tubulin] increases, and depends on the number of stable

177 ssion correlated to a decrease of acetylated tubulin induced by tau overexpression in N2a cells.

178 TC5 mutants incapable of ribosome or nascent tubulin interaction abolished tubulin autoregulation and

179 Genetic and chemical perturbation of CPAP-tubulin interaction activates extra centrosomes to nucle

180 engthening the longitudinal gamma-/alphabeta-tubulin interaction.

181 Furthermore, tubulin interacts closely with Galpha(s), the G-protein

182 on and mass spectrometry analysis that alpha-tubulin interacts with galectin-8 during mitosis.

183 architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate micr

184 For example, tubulin is activated and deactivated for assembly by a g

185 specificity of taccalonolide binding to beta-tubulin is demonstrated by immunoblotting, which allows

186 lter the head binding and propel cargo along tubulin is supplied by ATP at a ring 1,500 amino acids a

187 o the heterogeneity of microtubule dynamics: tubulin isoform composition [9, 10] and post-translation

188 echanics are modulated by a cell's choice of tubulin isoforms and post-translational modifications, a

189 tructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provid

190 teomics assay reported an induction of alpha-tubulin K40 acetylation upon Trichostatin A stimulation

191 Tau tubulin kinase 2 (TTBK2) is a critical regulator of cili

192 Tau tubulin kinase 2 (TTBK2) is a key player in the cilium a

193 Reactivity to 4 antigens, vimentin, beta-tubulin, lamin A/C, and apolipoprotein L2, was significa

194 nomethyl auristatin E: MMAE; a high-affinity tubulin ligand).

195 Most bacteria divide by corralling the tubulin-like FtsZ protein to mid-cell, where it assemble

196 l protein, either the actin-like MreB or the tubulin-like FtsZ.

197 ) macro-chloroplast lines overexpressing the tubulin-like GTPase protein gene FtsZ1 from Arabidopsis

198 We show that gamma-tubulin localises asymmetrically to the somatic Golgi wi

199 allows live-cell super-resolution imaging of tubulin localization and motion with a resolution of ~30

200 that, during depression, membrane-localized tubulin maintains a lower acetylation state, permitting

201 Thus, membrane tubulin may play a role in mood disorders, which could b

202 This suggested that deacetylated-tubulin might be more prevalent in depression.

203 em data provide strong evidence for membrane tubulin modification leading to reduced efficacy of the

204 We use mass photometry to observe tubulin monomers and heterodimers in solution simultaneo

205 asexual reproduction and results in aberrant tubulin morphology suggesting protein aggregation.

206 e 3-kinase (PI3K) activity, indeed regulates tubulin mRNA stability via changes in microtubule dynami

207 that triggers cotranslational degradation of tubulin mRNAs in response to excess soluble tubulin.

208 tro, and expression of a rationally designed tubulin mutant with a mutation in the rigosertib binding

209 We conclude that the tubulin mutation severely impairs the central hearing pa

210 r binder as tyrosination sensor, a live-cell tubulin nanobody specific towards tyrosinated microtubul

211 pc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determi

212 nucleation requires assembly of 8 alphabeta-tubulins, nucleation from gamma-TuRC occurs efficiently

213 cKO oocytes either failed to organize alpha-tubulin or developed an abnormally small bipolar spindle

214 izing kinesins that preferentially bind free tubulin over microtubules.

215 0% mixture of acetylated/nonacetylated alpha-tubulin peptides in the background of human olfactory ne

216 Tubulins play crucial roles in cell division, intracellu

217 l complex harboring a conoid, made of unique tubulin polymer fibers.

218 in vitro, while Augmin alone does not affect Tubulin polymerisation dynamics, it stimulates gamma-TuR

219 Here we traced the waves of tubulin polymerization and depolymerization that occur a

220 ing site in tubulin protein was confirmed by tubulin polymerization assay and molecular modeling.

221 independent tubulin-binding domain that has tubulin polymerization capacity.

222 onstant (K(d)) 0.4 +/- 0.1 muM] and inhibits tubulin polymerization in vitro; 4) had no effect upon t

223 e successful synthesis of the drug molecule "tubulin polymerization inhibitor" free from trace metal

224 y a specific marker for Golgi outposts-TPPP (tubulin polymerization promoting protein)-that we use to

225 XMAP215/Stu2/Alp14 accelerates tubulin polymerization while processively tracking micro

226 The most active compounds inhibited tubulin polymerization, with IC(50) values of 1.9-8.2 mu

227 gh transcriptome profiling, we show that the tubulin polymerization-promoting protein (TPPP) ringmake

228 EBV-miR-BART12 binds to the 3'UTR region of Tubulin Polymerization-Promoting Protein 1 (TPPP1) mRNA

229 roximately 5% ON01500, a potent inhibitor of tubulin polymerization.

230 nticancer activity of novel congeners of the tubulin-polymerizing molecule (+)-discodermolide.

231 Microtubules are dynamic tubulin polymers responsible for many cellular processes

232 ngths in dynamically unstable populations of tubulin polymers.

233 A new study reveals how differences in tubulin populations between two related Xenopus frog spe

234 n is dependent upon changes in the status of tubulin post-translational modifications indicative of h

235 biochemical forms in different cells due to tubulin posttranslational modifications (PTMs).

236 l properties of GTP- and GDP-bound alphabeta-tubulin predict the concentration dependence of microtub

237 analog capable of trapping Ser239 in beta(3) tubulin, presumably as a hemiacetal.

238 Finally, we show that XMAP215 and gamma-tubulin promote alphabeta-tubulin assembly in an additiv

239 -8 binding to the colchicine-binding site in tubulin protein was confirmed by tubulin polymerization

240 in human neuronal migration disorders encode tubulin proteins and a variety of tubulin-binding and -r

241 ofactor-dependent conformational dynamics of tubulin proteins.

242 ssion while target and reference (histone or tubulin) proteins were marked by fluorescent protein-tag

243 ts into biological functions of the cellular tubulin PTM "code."

244 Tubulin PTMs are known to affect microtubule stability,

245 re exists no tool that can specifically mark tubulin PTMs in living cells, thus severely limiting our

246 Here, we show that tubulins purified from two closely related frogs, Xenopu

247 n the contribution of other HDAC6 domains on tubulin recognition.

248 a new role for Gravin in coordinating gamma-tubulin recruitment during mitosis and illuminates the m

249 Complexes of Mto2 and Mto1 with gamma-tubulin regulate microtubule assembly.

250 ion of this machinery are coordinated by the tubulin-related GTPase FtsZ, which was found to form tre

251 mination of the relative contribution of key tubulin residues and taccalonolide moieties for drug-tar

252 acking AC9 fail to successfully assemble the tubulin-rich core of their apical complex, called the co

253 While the MT nucleator, gamma-tubulin ring complex (gamma-TuRC) has been identified, p

254 The gamma-tubulin ring complex (gamma-TuRC) is an essential regula

255 that besides the microtubule nucleator gamma-tubulin ring complex (gamma-TuRC), the branching effecto

256 correctly localising the MT nucleator, gamma-Tubulin Ring Complex (gamma-TuRC), within the cell.

257 The gamma-tubulin ring complex (gammaTuRC) is a microtubule nuclea

258 The gamma-tubulin ring complex (gammaTuRC) is the major microtubul

259 art, MAP20 does not cooperate with the gamma-tubulin ring complex in microtubule nucleation.

260 Mukherjee and Conduit introduce gamma-tubulin ring complexes (gamma-TuRCs), multi-protein comp

261 In contrast to textbook models of tubulin self-assembly, we have recently demonstrated tha

262 In dynamin 1-depleted MPCs by RNAi, alpha-tubulin showed a dispersed linear filament-like localiza

263 ssed nonsuicides, plasma membrane-associated tubulin showed significant decreases in acetylation from

264 ing a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion,

265 fied tetratricopeptide protein 5 (TTC5) as a tubulin-specific ribosome-associating factor that trigge

266 , what is different between the GTP- and GDP-tubulin states that enables microtubule growth and short

267 ng a microtubule-destabilizing mutation in a tubulin subunit.

268 teady state in mass, leaving enough of their tubulin subunits soluble to allow rapid growth and turno

269 otubules, which are cytoskeletal polymers of tubulin subunits.

270 lines harboring a Thr238Ala mutation in beta-tubulin sufficient to induce compound resistance.

271 py (cryo-EM) structures reveal the essential tubulin tail glutamates gripped by a double spiral of el

272 se-driven, ratchet-like translocation of the tubulin tail through the pore.

273 In particular, the precise role of tubulin tails and tail modifications in the diffusion pr

274 shed light on the roles played by disordered tubulin tails and tail modifications in the molecular me

275 Here, we show that beta-tubulin tails are necessary and sufficient for severing.

276 al role in protein diffusion; the disordered tubulin tails enhance affinity but slow down diffusion,

277 hat polyglutamylation and polyglycylation of tubulin tails lead to slower protein diffusion along MTs

278 carcinoma xenografts and the combination of tubulin-targeting agent paclitaxel with the BCR-ABL inhi

279 ubtle changes in the pose of binding to beta-tubulin that could account for the improved anticancer a

280 ubules are cylindrical polymers of alphabeta-tubulin that play critical roles in fundamental processe

281 was rescued by concurrent reduction of gamma-tubulin, the core microtubule nucleation protein.

282 ild-type MT is performed in steps of dimeric tubulin, the removal of the tails results in a step of m

283 Dynein binds to tubulin through two coiled coil stalks and a stalk head.

284 abolite that binds to the colchicine site of tubulin to induce mitotic arrest through a microtubule c

285 e by addition of bent guanosine triphosphate tubulin to the tips of curving protofilaments.

286 astin utilizes a hand-over-hand mechanism of tubulin translocation and microtubule remodeling.

287 We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progeni

288 a high activation energy barrier in lateral tubulin-tubulin interactions.

289 -terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Delta2 cleavage, two post-trans

290 Experimental manipulation of tubulin tyrosine ligase (TTL) or carboxypeptidase (Vasoh

291 Glutamylation, introduced by tubulin tyrosine ligase-like (TTLL) enzymes, is the most

292 ylation levels in cells by western blotting (tubulin vs histone acetylation), and by assessing their

293 with probe competition, indicating that beta-tubulin was the functional target.

294 ed by the fact that physiologically relevant tubulins were not available.

295 nds preferentially to microtubules over free tubulin, which contrasts with microtubule-depolymerizing

296 a direct link between the MEKK1 protein and tubulin, which is likely to be relevant to cancer cell m

297 Of note, acetylation of alpha-tubulin, which maintains microtubule flexibility and sta

298 X2 phase separates into a co-condensate with tubulin, which mediates microtubule nucleation in vitro

299 Using mutated human tubulin with blocked GTP hydrolysis, we demonstrate that

300 To contrast the binding of B2 versus DDM in tubulin, X-ray crystallography studies revealed a shift