ライフサイエンスコーパス: two-color

1 ue, or nearly dark, thus alternating between two colors.

2 e generalized to colocalization of more than two colors.

3 Ds) in living yeast and mammalian cells with two colors.

4 n microscopy imaging in three dimensions and two colors.

5 ackground heterogeneity relative to a proper two-coloring.

6 image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed a

7 t emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells.

8 lls encapsulated in collagen gels, obtaining two-color 4D super-resolution data sets spanning tens of

9 Together these two reagents enable two-color activation of neural spiking and downstream sy

10 sensory experience, performing simultaneous two-color activity recording, and applying optical pertu

11 ication of adjacent primer pairs, labeled in two colors, allowed us to narrow the 4q35.1 breakpoint t

12 Fluorescent two-color analysis of Gag-TC in HeLa cells revealed that

13 index unit (RIU) by using a self-referenced two-color analysis.

14 from a global maximum likelihood analysis of two-color and three-color photon trajectories.

15 These methods are directly applicable to two-color antibody arrays.

16 essentially equivalent between the one- and two-color approaches and strongly suggest that this vari

17 compared with ten other body tissues using a two color array experimental design.

18 on is a promising method for the analysis of two color array experiments that compare multiple sample

19 We show how this improved the performance of two-color array analysis for arrays for genomic analysis

20 We describe a method for analyzing two-color array data to efficiently measure the differen

21 A two-color array of 20 excitation and deactivation beams

22 However, for data from two-color arrays, tests for differential expression may

23 ting different analysis methods designed for two-color arrays.

24 echnologies, such as single-color arrays and two-color arrays.

25 itu hybridization (PNA FISH) method, a rapid two-color assay for detection of C. albicans and C. glab

26 The second application is a two-color assay for the specific detection of the A-to-T

27 the two types of QD-probe conjugates in the two-color assay format is advantageous to maximize assay

28 s been combined with a cytokine Elispot in a two-color assay to confirm that cytotoxicity and interfe

29 Using a two-color assay to simultaneously monitor poly(A) tail r

30 at concentrations as low as 100 fM using the two-color assay.

31 4-fold increase in throughput over standard two-color assays.

32 Two-color Blink determined that different CTLs rarely oc

33 Our approach highlights how two-color calcium imaging can help identify and localize

34 elengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-b

35 ed for three-color whole-body imaging of the two-color cancer cells interacting with the GFP-expressi

36 ensitivity SHAPE experiments, we developed a two-color capillary electrophoresis approach with zeptom

37 large amount of research has been devoted to two-color cDNA arrays to improve experimental design, no

38 oligonucleotide arrays, each hybridized with two-color cDNA samples derived from 20 different human t

39 We report a two-color, cell-based screen to identify specific recept

40 learned a color change-detection task where two colored circles (selected from a 4-color set) were p

41 uorescence imaging readily distinguished the two color-coded cell lines and their different abilities

42 riate thresholds for the analysis of sets of two-color coincidence data.

43 sing a simple microfluidic device to perform two-color coincidence detection (TCCD) and single pair F

44 the method of single-molecule fluorescence, two-color coincidence detection (TCCD) to detect coincid

45 we have been able to employ single-molecule two-color coincidence detection (TCCD) to observe direct

46 Two-color coincidence detection is a single-molecule flu

47 listic diagnostic applications, we show that two-color coincidence detection of single-stranded DNA m

48 This method should allow the application of two-color coincidence detection to more complex biologic

49 es can be directly counted without employing two-color coincidence detection.

50 Examples of suitable dyes for such two-color colocalization include Oregon Green or Alexa F

51 each virus using three different colors and two-color combinatorial labeling.

52 It is perceived when two color cone types in the retina (blue and red) with n

53 m quantitative statistical colocalization on two-color confocal images, specifically of plant cells.

54 Two-color confocal microscopy indicated that ErbB2 is co

55 Combining SaCas9 and SpCas9, we demonstrated two-color CRISPR imaging with the capability to resolve

56 we report the development of an alternative two-color CRISPR labeling method using only the well-cha

57 istics of the switch are characterized using two-color cytometry.

58 d SIM setup that is fast enough to record 3D two-color datasets of living whole cells.

59 their gaze in the direction opposite one of two color-defined targets.

60 iments typically use either a one-color or a two-color design to measure mRNA abundance.

61 Langerhans using a microfluidic system with two-color detection.

62 ake monomeric PAmCherry1 a preferred tag for two-color diffraction-limited photoactivation imaging an

63 We have recently combined two-color direct stochastic optical reconstruction micro

64 discriminate large T's from L's, or bisected two-color disks from their mirror images under the same

65 Two-color DNA microarray-based detection was demonstrate

66 ochromator and a downstream chicane enables "two-color" double pulses with femtosecond duration and t

67 For data from a two-color dye swap experiment, the one-sample test appea

68 dyes or tautomeric benzofluorescein class of two-color dyes.

69 ounds surveyed, five displayed well-resolved two-color emission in solvents as nonpolar as hexane.

70 Using two-color evanescent field microscopy, we imaged the lip

71 luorescent proteins in different colors, and two-color evanescent-field microscopy was used to view s

72 different fluorophores in solution by using two-color excitation and detection of coincidence fluore

73 In the latter case, by using two color excitations, the conjugate could be simultaneo

74 A two-color experiment that made use of two different size

75 fewer arrays) will perform as efficiently as two-color experiments.

76 This higher resolution, two-color extension of the cPALM technique will expand t

77 ddress these issues, we have developed a new two-color Fab labeling method that allows two samples to

78 These results demonstrate that two-color FFS is a powerful tool for measuring dynamic p

79 Dual, two-color FISH staining revealed coinfection of numerous

80 hagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin

81 t 3 months posttransplant, was determined by two-color flow cytometric analysis of peripheral blood g

82 ercentage of donor T cells was quantified by two-color flow cytometric analysis using antibodies spec

83 l repertoire was evaluated in chimeras using two-color flow cytometry and monoclonal antibodies direc

84 luorescence of CaValpha2delta1, indicated by two-color flow cytometry assays and confocal imaging, an

85 cence of CaValpha2delta1 as characterized by two-color flow cytometry assays and confocal imaging; 2)

86 To overcome these limitations, we used two-color flow cytometry to identify and select microvas

87 cells expressing RANKL on their surfaces by two-color flow cytometry using FITC-conjugated osteoprot

88 le-cell transcriptomics coupled with dynamic two-color fluorescence are used by Gehart et al. (2019)

89 We have developed an in vitro, two-color fluorescence assay to monitor kinetics of sing

90 al neurons and astrocytes were assessed in a two-color fluorescence assay.

91 th a semiconductor quantum dot (QD)-mediated two-color fluorescence coincidence detection scheme.

92 We have used two-color fluorescence coincidence detection to directly

93 In two-color fluorescence correlation spectroscopy (TCFCS),

94 Fluorescence-based methods like two-color fluorescence cross-correlation spectroscopy ca

95 , raster image correlation spectroscopy, and two-color fluorescence cross-correlation spectroscopy.

96 re achieved by utilizing a CE-LIF array with two-color fluorescence detection.

97 ADCP was measured by two-color fluorescence flow cytometry using PKH2 (green

98 receptor subtypes and their G-proteins using two-color fluorescence fluctuation spectroscopy (FFS) of

99 ip and clinical significance, we developed a two-color fluorescence in situ hybridization (FISH) tech

100 apex of amplification were used to develop a two-color fluorescence in situ hybridization assay and a

101 Two-color fluorescence in situ hybridization was consist

102 ecture of human kinetochores, we developed a two-color fluorescence light microscopy method that meas

103 Similar to two-color fluorescence measurements, multiple enzyme lab

104 Two-color fluorescence microscopy confirmed that both un

105 nules (CGs)) on the single-particle level by two-color fluorescence microscopy in a time-resolved man

106 PIE-FCCS is a two-color fluorescence microscopy method that is directl

107 We use an in vivo two-color fluorescence microscopy technique to determine

108 , were quantified in a blinded fashion using two-color fluorescence microscopy.

109 surfaces at the single-particle level using two-color fluorescence microscopy.

110 Here we describe an improved two-color fluorescence nanoscopy technique that can achi

111 This assay requires analyzing two-color fluorescence patterns in yeast colonies, which

112 Binding affinity was quantified by two-color fluorescence ratio imaging of cells stained wi

113 We developed a two-color fluorescence reporter assay in Escherichia col

114 labeled vesicles, were detected by measuring two-color fluorescence signals simultaneously.

115 We detect this encoded two-color fluorescence signature of each target by perfo

116 inct padlock probes, each rendering a unique two-color fluorescence signature to a nucleic acid targe

117 ere observed, each exhibiting characteristic two-color fluorescence signatures: 1) primary fusion, in

118 tiple CpG island loci was then detected in a two-color fluorescence system.

119 Our two-color fluorescence-activated cell sorting-based scre

120 efficiency, high-throughput and quantitative two-color fluorescence-activated cell sorting-based scre

121 g green fluorescent protein were isolated by two-color fluorescence-activated cell sorting.

122 Incorporation of two colored fluorescent probes in each of the components

123 n microarrays, we trained neural networks on two-color fluorescent detection of serum IgG binding and

124 We synthesized a two-color fluorescent dextran to measure ASL [K(+)], TAC

125 oli mRNAs at single-gene resolution by using two-color fluorescent DNA microarrays.

126 We report a new two-color fluorescent imaging system to visualize the mo

127 Two-color fluorescent in situ hybridization on dissociat

128 Elapsed time was measured with a two-color fluorescent marker gene that converts from exp

129 errations, which is commonly achieved with a two-color fluorescent oligonucleotide probe (TaqMan) des

130 We have developed a two-color fluorescent reporter for cellular assays of ex

131 We performed transposon mutagenesis of a two-color fluorescent reporter strain to identify new re

132 We have constructed two-color fluorescent reporter strains in which ompF and

133 Using two-color fluorescent repressor-operator systems and a s

134 A simple, two-color fluorescent sample labeling and detection stra

135 We have employed a two-colored fluorescent reporter system to isolate FHF a

136 cceptors absent or photobleached, from which two-color FRET data is collected in the same experiment.

137 The two colors from Alexa Fluor 532 and YOYO-3 were disperse

138 e faster and more accurate discrimination of two colors from different categories than two colors fro

139 of two colors from different categories than two colors from the same category, even when same- and d

140 However, mesopic and dark-adapted two-color fundus-controlled perimetry (FCP, also called

141 Our approach is based on the expression of a two-color fusion protein in a vector that allows the ins

142 ection of circulating bacteria cells (CBCs), two-color gold and multilayer magnetic nanoparticles wit

143 ects of background heterogeneity on properly two-colored graphs to those with alternative schemes in

144 ssay format, we demonstrate development of a two-color homogeneous electrophoretic immunoassay for co

145 esis and without byssogenesis were used in a two-color hybridization to reveal the gene differential

146 We have extended these tools for analysis of two-color images to resolve heteromeric interactions bet

147 Using a two color imaging approach that allows tracking of singl

148 Using two-color imaging and fast extracellular solution change

149 pands the scope of biotin ligase labeling to two-color imaging and yeast-based applications.

150 form two-color live-cell STED microscopy and two-color imaging in vivo.

151 pHuji plus SEP pair, we perform simultaneous two-color imaging of clathrin-mediated internalization o

152 Two-color imaging of epidermal growth factor (EGF) ligan

153 Two-color imaging of tethered vesicles with Myo2p, the G

154 The method is based on two-color imaging of the relaxation of the protein conce

155 We demonstrate a two-color imaging system with single-molecule resolution

156 s make PATagRFP an excellent protein tag for two-color imaging techniques, including conventional dif

157 Simultaneous two-color imaging was used to correlate changes in endos

158 to cell-based therapies for retinal disease, two-color imaging was used to localize transplanted cell

159 Using two-color imaging we demonstrate co-transport of Rab11A

160 iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cel

161 ble with signal-amplification techniques and two-color imaging.

162 Moreover, confocal microscopy combined with two color immunofluorescence showed co-localization of t

163 Frozen sections were stained by two-color immunofluorescence for C4d, type IV collagen a

164 ned antigen-coated particles, as revealed by two-color immunofluorescence microscopy, and these cells

165 Two-color immunofluorescence revealed that one-third of

166 for several latent and lytic transcripts and two-color immunofluorescence staining to analyze express

167 Two-color immunofluorescent labeling of TLR2 and cytoker

168 biocytin-labeled PNs were visualized with a two-color immunoperoxidase procedure using nickel-enhanc

169 Two-color in situ analysis revealed that almost all HS3S

170 y-ionization mass spectrometry combined with two-color infrared photodissociation (IRPD) spectroscopy

171 The measurements are carried out by applying two-color, infrared-infrared photodissociation mass spec

172 Using two-color interphase fluorescence in situ hybridization

173 at the air-water interface using femtosecond two-color IR-pump/vibrational sum-frequency probe spectr

174 of about 10 s, evidenced by isomer-selective two-color (IR-IR) spectroscopy.

175 Here, we demonstrate a two-color irradiation scheme whereby initiating species

176 By merging two-color iSPIM with automated lineaging techniques we r

177 ltaneous comparison of results from one- and two-color labeling designs, using two independent RNA sa

178 Here we demonstrate specific and orthogonal two-color labeling of repetitive sequences in living hum

179 Using two-color labeling, colocalization of both components of

180 Colocalization of two-color labels using wide-field microscopy is a method

181 hollow waveguide using synthesized few-cycle two-color laser waveforms.

182 r, we present a novel assay scheme that uses two-color latex labels for rapid multiplex detection of

183 eneous mammalian samples using a ratiometric two-color lectin microarray approach.

184 stinct monomeric NIR FP variants, we perform two-color live-cell STED microscopy and two-color imagin

185 Furthermore, using two-color, live-cell superresolution cross-correlation s

186 We applied our two-color localization method to uncover an unexpected ~

187 ifferential expression more efficiently than two-color loop designs.

188 e-color loop designs are more efficient than two-color loop designs.

189 by simulation and compared to several other two-color microarray designs.

190 Two-color microarray experiments in which an aliquot der

191 In two-color microarray experiments, well-known differences

192 llows users to find optimal loop designs for two-color microarray experiments.

193 A primary reason for using two-color microarrays is that the use of two samples lab

194 or microarrays and the ratio error model for two-color microarrays or ratios built from two single-co

195 Three-color microarrays, compared with two-color microarrays, can increase design efficiency an

196 correction of cross-channel correlation for two-color microarrays, identification of genes specific

197 Using two-color microarrays, we analyzed the transcriptomes of

198 tent of probe-specific dye and slide bias in two-color microarrays.

199 e edited region in the genome in between the two color moieties.

200 riment varying the relative frequency of the two color morphs of D. sambucina to test whether rare mo

201 The results demonstrate the utility of the two-color MRP1 construct for investigating ATP-binding c

202 Two-color MRP1 transport activity was normal, as shown b

203 y MRP1 structural dynamics, we engineered a "two-color MRP1" construct by fusing green fluorescent pr

204 tion, we coupled our quenching approach to a two-color NA assay with correlated, two-color, single-mo

205 Here, we use two-color near-field ultrafast electron microscopy to ph

206 Herein we report a paper-based two-color oligonucleotide detection assay with tunable s

207 sion of both sensors in a single cell allows two-color optical control of transcription both in batch

208 aged biomolecules at 900 nm may allow facile two-color optical interrogation of bimodal signaling pat

209 The model is experimentally confirmed using "two-color" optical spin noise spectroscopy on a mixture

210 asy-to-use method to evaluate the quality of two-color (or more) fluorescence coincidence measurement

211 tes as high as 4 s in one color and 8.5 s in two colors over tens of time points.

212 Two-color PALM shows that the freely diffusible mitochon

213 Here, we report the use of two-color photoactivated localization microscopy (PALM)

214 super-resolution techniques such as one- and two-color photoactivated localization microscopy (PALM).

215 ility, we paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging

216 We used single and two-color photoactivated localization microscopy to stud

217 One version of the two-color photoanode, based on an organic push-pull dye

218 t a new and versatile method for fabricating two-color photoanodes featuring spatially isolated chrom

219 Sequenced two-color photoexcitation permits the sub-picosecond mod

220 We performed a two-color photon echo experiment on a bacterial reaction

221 Here, we show that a set of two-color photon-echo experiments performs quantum state

222 Working with a two-color platform, this study uses ten spike-in microar

223 wn prospectively, has not been available for two-color platforms.

224 ed "continuously red-photoactivatable green" two-color probe for live cell imaging.

225 ce in situ hybridization was performed using two-color probes for BCL6, MYC, IGH-BCL6, and del(6)(q22

226 gene knockdown studies, as demonstrated in a two color proof-of-principle study with two biological t

227 of different AMPA receptor subunits by using two-color pulse-chase labeling.

228 y be drastically increased with an optimized two-color pulse.

229 ingle-color pump and 24th (30 eV) in case of two-color pump.

230 Two-color quenched probe pairs were then tested in the d

231 G1, S, and G2, while Fucci(SCA) permitted a two-color readout of G1 and S/G2.

232 ) at nascent endocytic sites was revealed by two-color real-time fluorescence microscopy; the type I

233 l is studied using DC slice ion imaging with two-color reduced-Doppler detection at 243 nm and 212 nm

234 This method provides an ability to evaluate two-color registration compatible with the scale of mole

235 Using a combination of two-color resonant two-photon ionization (2C-R2PI), lase

236 wn that the fingerprints labeled with one or two colors, resulting in 40-70 bands per clone, were ass

237 were probed in replicate experiment sets by two-color, rolling circle amplification on microarrays c

238 nd plots BAF values for all samples, using a two-color scheme for intuitive visualization.

239 Drosophila relies on the comparison between two color-sensitive photoreceptors, R7 and R8.

240 the translocation step we have extended our two-color single molecule fluorescence coincidence metho

241 We applied two-color single quantum dot tracking to quantify recept

242 This analysis uses two-color single-frame images to provide a systems-level

243 We demonstrated two-color single-molecule imaging by observing the spati

244 imaging microscopy (FRET/FLIM) combined with two-color single-molecule tracking to study the effect o

245 ole-genome genotyping (WGG) assay based on a two-color, single-base extension (SBE), single-nucleotid

246 ach to a two-color NA assay with correlated, two-color, single-molecule fluorescence detection.

247 evice, and measuring the extent of mixing of two colored solutions under different angles and at flow

248 spatial intensity distribution analysis and two-color spatial cumulant analysis (2c-SpCA).

249 We call these new methods two-color spatial intensity distribution analysis and tw

250 Two-color spatio-temporal image cross-correlation spectr

251 Using simultaneous two-color spinning-disk confocal microscopy, we monitor

252 The discussion focuses on two-color spotted cDNA microarrays, but many of the same

253 intralaboratory quality control protocol for two color, spotted microarrays was developed using cDNA

254 Two-color sptPALM imaging was demonstrated using several

255 Following training, the women were shown two colored squares and asked to choose the one with the

256 Two-color staining showed that those cells that were abl

257 bsorption bands in the visible region of the two colored states of the molecular switch.

258 We also find that bulk, two-color, static quenching FRET experiments are best su

259 Two-color stimulated-emission-depletion microscopy indic

260 ipants first learned to discriminate between two color stimuli, one paired with an electrical shock (

261 nderstand this process, we developed a novel two-color structured illumination microscopy with single

262 io-orthogonal labeling strategy that enables two-color super-resolution (STED) and 3D confocal imagin

263 ectrally distinguishable probes, we obtained two-color super-resolution images of mitochondria and th

264 directly test this model, we carried out 3D, two-color super-resolution microscopy of histones and DN

265 es also facilitate acquisition of live-cell, two-color, super-resolution images, expanding the utilit

266 As a result, we have achieved routine two-color superresolution imaging and single-molecule de

267 Two-color superresolution imaging demonstrated colocaliz

268 compatible with time-lapse and simultaneous two-color superresolution imaging in living cells.

269 Using two-color superresolution imaging, we observed that the

270 Two-color superresolution localization microscopy confir

271 teins in T and HeLa cells using quantitative two-color superresolution localization microscopy.

272 psin-Hsc70 coclusters are also visualized by two-color superresolution microscopy.

273 )C SWNT isotopes, we demonstrate multiplexed two-color SWNT Raman-based protein detection.

274 ew self-assembly mode can be considered as a two-color symmetry-broken double gyroid morphology, prov

275 Using a two-color system to visualize pairs of chromosome loci i

276 for multiplex detection beyond the available two-color systems.

277 To answer this, we let observers search for two colored targets among distractors.

278 r the S-cone pathway (isolated using Stiles' two-color threshold method) for the same locations.

279 Using elegant two-color time-lapse imaging, the authors showed that mi

280 nd comove with KIF5B along axons revealed by two-color time-lapse imaging.

281 We examined the feasibility of using a two-color time-resolved detection scheme with microdevic

282 We report on the design and performance of a two-color, time-resolved detector for the acquisition of

283 However, two-color TIRF microscopy using fluorescent proteins fus

284 Furthermore, two-color TIRFM showed that Rab11a-mCherry was present i

285 Furthermore, two-color total internal reflection fluorescence (TIRF)

286 Here we image with two-color total internal reflection fluorescence microsc

287 mentation assays and direct visualization by two-color total internal reflection fluorescence microsc

288 munostaining of the endogenous proteins, and two-color total internal reflection fluorescence structu

289 plate was sequenced on the microchip using a two-color two-lifetime format with POP-6 as the sieving

290 base flipping using a microfluidic mixer and two-color two-photon (2c2p) fluorescence lifetime imagin

291 Using in vivo two-color two-photon imaging with genetically encoded ca

292 hermore, using a complementary SR technique, two-color, two-dimensional stimulated emission depletion

293 ced in a single capillary gel column using a two-color, two-lifetime format.

294 We used two-color, two-photon uncaging to fire and block action

295 We prove that all properly two-colored, undirected, regular graphs are evolutionari

296 The two colors were either from the same or different catego

297 Two colors were successively presented within a block of

298 A two-color Western blotting technique is used to determin

299 Russian speakers were faster to discriminate two colors when they fell into different linguistic cate

300 By means of two-color z-scan fluorescence correlation spectroscopy,