ライフサイエンスコーパス: ubiquitylated

1 effector-binding loop, and is GTP-bound and ubiquitylated.

2 n E3 ligase MDM2 and, upon DNA damage, is de-ubiquitylated.

3 on and have mapped the sites on which LAT is ubiquitylated.

4 all three subunits of the 9-1-1 complex are ubiquitylated.

5 CRL4(Cdt2) is recruited and the substrate is ubiquitylated.

6 he rate at which the transcription factor is ubiquitylated.

7 n to cause hamster NAT2 to be constitutively ubiquitylated.

8 y, but not all, UIM-containing proteins were ubiquitylated.

9 tment was able to interact with MDM2 and was ubiquitylated.

10 ro but only when NRF1 protein is highly poly-ubiquitylated.

11 ifying factor that recruits the target to be ubiquitylated.

12 is revealed that H2AX is both sumoylated and ubiquitylated.

13 levels of polysumoylated Nrf2 that was also ubiquitylated.

14 l kinase RIOK3 specifically recognizes these ubiquitylated 40S ribosomes through a unique ubiquitin-i

15 RIOK3 induces the degradation of ubiquitylated 40S ribosomes through progressive decay of

16 RPA is hyper-phosphorylated and ubiquitylated after DNA damage.

17 iochemical evidence indicating that HtsRC is ubiquitylated and degraded by the proteasome.

18 nitially PARylated by PARP1 and subsequently ubiquitylated and degraded by the proteasome.

19 tb1p and Btb2p are stable proteins, Btb3p is ubiquitylated and degraded in a Pcu3p-dependent manner.

20 Upon completion of MII, Isc10 is ubiquitylated and degraded in an AMA1-dependent manner,

21 , PIF3 was also rapidly phosphorylated, poly-ubiquitylated and degraded in response to blue light.

22 ow that fission yeast S phase cyclin Cig2 is ubiquitylated and degraded via both the SCF and the APC/

23 ek2A, which directly binds the APC/C, can be ubiquitylated and destroyed in Fizzy/Cdc20-depleted Xeno

24 Emi1 accumulates before mitosis and is ubiquitylated and destroyed in mitosis, independent of t

25 s carbon source transporters are known to be ubiquitylated and endocytosed when glucose-starved cells

26 The mutant receptors were poorly ubiquitylated and exhibited decreased association with t

27 the absence of Pma1-D378N, wild-type Pma1 is ubiquitylated and it undergoes internalization from the

28 of the activity trajectories originated from ubiquitylated and non-ubiquitylated FBH1 molecules revea

29 ve for ATP-dependent in vitro degradation of ubiquitylated and non-ubiquitylated proteins and did not

30 hat neuronal nitric-oxide synthase (nNOS) is ubiquitylated and proteasomally degraded.

31 nant catalytic light chain (LC) of BoNT/E is ubiquitylated and rapidly degraded in cells.

32 le-stranded DNA beyond the break and is then ubiquitylated and removed from chromatin by the same pat

33 ied proteomes using parallel quantitation of ubiquitylated and SUMOylated peptides.

34 compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to gen

35 phyA is ubiquitylated and targeted for proteasome-mediated degra

36 H2B was shown to be ubiquitylated and then deubiquitylated at the GAL1 core

37 CD80, CTLA-4 remained ligand bound, and was ubiquitylated and trafficked via late endosomes and lyso

38 howed that the mutant protein was apparently ubiquitylated and was preferentially located in the cyto

39 We now show that Pma1-D378N is ubiquitylated, and it heterooligomerizes with wild-type

40 p12(I) is ubiquitylated, and mutations of its unique carboxy-termi

41 a Derlin-1- and p97-dependent fashion, poly-ubiquitylated, and targeted for proteasomal degradation.

42 We show that both Phm5p and Cps1p are ubiquitylated, and that in a doa4 mutant, which has redu

43 find a pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub) outside of canon

44 of mouse glomeruli revealed that podocin is ubiquitylated at two lysine residues.

45 UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulati

46 R1 and AFB1-3, which indicates that they are ubiquitylated by a CUL1-dependent E3 ligase.

47 Here we report that RECQL4 is ubiquitylated by a mitochondrial E3 ligase, MITOL, at tw

48 be a substrate of AtCHIP, because it can be ubiquitylated by AtCHIP in vitro and because the activit

49 FtsH1 can be ubiquitylated by AtCHIP in vitro, and the steady-state l

50 In ERAD, membrane and luminal substrates are ubiquitylated by ER-resident RING-type E3 ubiquitin liga

51 tion of the APC, as the N-terminal domain is ubiquitylated by purified APC in vitro.

52 nts lacking IpaH1.4 expression are bound and ubiquitylated by RNF213 in the cytosol of IFNgamma-prime

53 Here, we show that the FACT subunit Spt16 is ubiquitylated by San1 (an E3 ubiquitin ligase) and degra

54 ogen synthase kinase 3 at serine 33 and then ubiquitylated by SCF(FBXW7) and degraded.

55 BOK is ubiquitylated by the AMFR/gp78 E3 ubiquitin ligase compl

56 During mitosis, CYCLIN A and CYCLIN B are ubiquitylated by the anaphase-promoting complex/cyclosom

57 omal H2A/H2B and H3/H4 complexes are broadly ubiquitylated by the APC/C in an unexpected manner.

58 roteins not involved in a repair complex are ubiquitylated by the E3 ubiquitin ligase CHIP and subseq

59 to be an hsp90 "client" protein that can be ubiquitylated by the hsp70-dependent E3 ubiquitin ligase

60 We show that MCM3 is ubiquitylated by the KEAP1-CUL3-RBX1 complex in cells an

61 Under basal conditions, NRF2 is continuously ubiquitylated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase

62 ion of Rtt101 or mutating H3 lysine residues ubiquitylated by the Rtt101(Mms1) ligase impairs nucleos

63 the G2 phase of the cell cycle, and CP110 is ubiquitylated by the SCF(Cyclin F) ubiquitin ligase comp

64 ort that the central player in HR, RAD51, is ubiquitylated by the SCF(FBH1) complex.

65 In general, G(1) and S phase cyclins are ubiquitylated by the SCF, whereas ubiquitylation of mito

66 alpha2) is a short-lived protein known to be ubiquitylated by two distinct pathways, one involving th

67 ere, we report that Ephexin5 can be directly ubiquitylated by UBE3A.

68 Both the internalization of ubiquitylated cargo from the plasma membrane and its sor

69 Ubiquitylated cargo proteins are recognized by ubiquitin

70 Epsin1 recruits ubiquitylated cargo to endocytic sites.

71 ted by ubiquitylation and, unlike sorting of ubiquitylated cargo, is insensitive to functional inhibi

72 o the class E compartment, which accumulates ubiquitylated cargoes in cells lacking ESCRT components.

73 action of UIM-containing proteins with other ubiquitylated cellular proteins.

74 quitylated CFTR, whereas p97 associated with ubiquitylated CFTR.

75 f its paralog RNF169, but how they recognize ubiquitylated chromatin is unknown.

76 ing of various cellular substrates including ubiquitylated chromatin proteins.

77 rgeted ATP-dependent segregase that extracts ubiquitylated client proteins from membranes, protein co

78 mpanied by a reduced ability of HSJ1 to bind ubiquitylated clients and to exert its chaperone activit

79 The Cdc48/p97 segregase then associates with ubiquitylated CMG, leading rapidly to helicase disassemb

80 In the absence of CRL2(Lrr1), Mcm7 is not ubiquitylated, CMG unloading is inhibited, and a large s

81 Ubiquitylated DAT was detected in the plasma membrane us

82 that the cellular levels of Pol beta and its ubiquitylated derivative are modulated by Mule and ARF a

83 diated through specific association with K63 ubiquitylated, detergent insoluble, higher order mutant

84 Mechanistically, RNF168 directly ubiquitylated DHX9 to facilitate its recruitment to R-lo

85 oluble) proteins showed they are extensively ubiquitylated during heat stress, especially in plants d

86 ion of E6 protein stability and suggest that ubiquitylated E6 could be a substrate for USP15 ubiquiti

87 Unmodified alpha2 also bound to and was ubiquitylated efficiently by Slx5-Slx8.

88 Expression of ubiquitylated Egfr (Egfr-Ub) was increased in Min/+ ente

89 strumental in the extraction and delivery of ubiquitylated ER proteins for proteasomal degradation.

90 quitylates ERCC1 in vitro, and the levels of ubiquitylated ERCC1 are markedly enhanced in USP45 knock

91 Reversal of these defects requires ubiquitylated FANCI-FANCD2.

92 tories originated from ubiquitylated and non-ubiquitylated FBH1 molecules revealed that ubiquitylatio

93 misfold and would need to be recognized and ubiquitylated for degradation.

94 clin-dependent kinase inhibitor p27(Kip1) is ubiquitylated for proteasomal degradation in Wnt10b-indu

95 ng domain provides enhanced affinity for the ubiquitylated form of PCNA and is essential for in vivo

96 ubstrate Sic1 stabilized Sic1 in vivo in its ubiquitylated form.

97 the design of biosensors for specific (poly)ubiquitylated forms of individual substrates.

98 ed in response to DNA damage, and to isolate ubiquitylated forms of RNA polymerase II.

99 -course experiments reveal that the non- and ubiquitylated forms of the Cat-domain are stable in cell

100 n WT mice, coincident with the appearance of ubiquitylated forms of the protein.

101 preferential physical interactions with the ubiquitylated forms of the replicative sliding clamp, pr

102 rotein (VCP)/p97 promotes the degradation of ubiquitylated GS, resulting in its accumulation in cells

103 ting a repressive histone modification, mono-ubiquitylated H2A (H2Aub), during meiosis.

104 xpression of Ph with an intact SAM increases ubiquitylated H2A in cells.

105 nstrated that the HoxC5 gene is regulated by ubiquitylated H2A in HeLa cells and that ubiquitylated H

106 by ubiquitylated H2A in HeLa cells and that ubiquitylated H2A is localized on 5' regulatory regions

107 BCOR complex components and mono-ubiquitylated H2A localize to BCL6 targets, indicating t

108 We establish that RNF169 binds to ubiquitylated H2A-Lys13/Lys15 in a manner that involves

109 s distinct from that by which 53BP1 binds to ubiquitylated H2A-Lys15 highlighting the diversity in si

110 We additionally show that both Rad6 and ubiquitylated H2B are absent from two regions of transcr

111 region, and Spt16 regulates the formation of ubiquitylated H2B both globally and at the GAL1 gene.

112 35 into COMPASS, and increasing the level of ubiquitylated H2B in H2A tail mutants suppresses the H3K

113 ition of p97 function affected the amount of ubiquitylated H2B in muscle cells.

114 ombinant chromatin template containing fully ubiquitylated H2B to gain valuable insights.

115 Here we demonstrate, using chemically ubiquitylated H2B, a direct stimulation of hDot1L-mediat

116 used uH2A, as well as a previously generated ubiquitylated H2B, in chaperone-coupled nucleosome stabi

117 ne-associated and overall cellular levels of ubiquitylated H2B.

118 ransferase DNMT1, a module known to bind the ubiquitylated H3 (H3Ub), as a specific reader for H3K9me

119 y for the semisynthesis of site-specifically ubiquitylated histone H2A (uH2A).

120 cruited to sites of DNA damage by binding to ubiquitylated histone H2A.

121 2 promoter and hydrolyzes monoubiquitin from ubiquitylated histone H2B and blocks transcription of th

122 ays to demonstrate that the direct effect of ubiquitylated histones on nucleosomal stability is in fa

123 lymerase beta (Polbeta), crucial for BER, is ubiquitylated in a BER complex-dependent manner by TRIP1

124 , bacterially expressed CYP2E1(Delta3-29) is ubiquitylated in a CHIP-dependent manner when it is incu

125 olog, budding yeast Polymerase eta itself is ubiquitylated in a manner dependent on its ubiquitin-bin

126 slation in vitro, ribosome-stalled NSPs were ubiquitylated in an Ltn1-dependent manner, while still r

127 w that the TCR:CD3 complex is constitutively ubiquitylated in immature double positive (DP) thymocyte

128 ted in plants (EIN3 and JAZ6) or shown to be ubiquitylated in other eukaryotes (ribosomal subunits, e

129 nly a few have been conclusively shown to be ubiquitylated in planta.

130 ded those predicted by genetic studies to be ubiquitylated in plants (EIN3 and JAZ6) or shown to be u

131 study revealed over 1,200 proteins that were ubiquitylated in primary mouse CD4(+) T cells and highli

132 a model in which only a fraction of pol II, ubiquitylated in response to cisplatin damage of DNA, di

133 resin to show that the Def1 protein becomes ubiquitylated in response to DNA damage, and to isolate

134 ngly, tau (full-length or truncated) was not ubiquitylated in situ.

135 Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating

136 ed E3-ligase activity in vitro and also self-ubiquitylated in the presence of ubiquitin-activating an

137 in isoforms that are both phosphorylated and ubiquitylated in the yeast Saccharomyces cerevisiae, ide

138 Specifically, we show that Siz1 can be ubiquitylated in vivo and is degraded in an Slx5-depende

139 Here we show that FRQ is likely ubiquitylated in vivo, and its proper degradation requir

140 substrates, the UBR1/2-bound RECQL4 was not ubiquitylated in vivo, and was a long-lived protein in H

141 ly phosphorylated forms of Zip1, and Zip1 is ubiquitylated in vivo, indicating that Zip1 is a substra

142 demonstrate that TIN2 binds to Siah2 and is ubiquitylated in vivo.

143 (Cdk9), the catalytic subunit of P-TEFb, is ubiquitylated in vivo.

144 ed polyglutamine diseases is the presence of ubiquitylated inclusion bodies, which sequester various

145 retained in the ER and can form cytoplasmic ubiquitylated inclusions.

146 Substrates in rad4Delta cells are ubiquitylated, indicating that Rad4 regulates a postubiq

147 ytes was associated with the accumulation of ubiquitylated intermediates of troponin I and was protea

148 d knockdown of USP8 enhanced accumulation of ubiquitylated KCa3.1, thereby inhibiting channel degrada

149 te levels of LRP6 and increases the level of ubiquitylated LRP6.

150 Arginine substitution of the ubiquitylated lysine impairs this inactivation mechanism

151 We identified three ubiquitylated lysine residues and showed that DNA ligase

152 hibition of proliferation and replacement of ubiquitylated lysine residues reduced the in vitro ubiqu

153 lation of ubiquitylated yeast SOD1 and other ubiquitylated mammalian proteins activate ExoU.

154 nherent to Cdc48, which specifically unfolds ubiquitylated Mcm7 and thereby disassembles CMG.

155 Ubiquitylated MDC1 associates with chromatin before and

156 m arrangement of UBDs is required to protect ubiquitylated Met4 from degradation and enables direct i

157 Once internalized, ubiquitylated MHC class I is targeted to the late endocy

158 t of p21WAF1/CIP1 turn-over and show how non-ubiquitylated molecules might bypass the 19S regulator o

159 is phosphorylated by ATM and interacts with ubiquitylated MRE11 to mediate early steps of homologous

160 agy receptor, TAX1BP1, mediates clearance of ubiquitylated Mtb and targets bacteria to LC3-positive p

161 eir tethered nascent chains, and deliver the ubiquitylated nascent chains to the proteasome.

162 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex, with

163 esolution model of human RNF169 binding to a ubiquitylated nucleosome, and validate it by electron cr

164 he diversity in site-specific recognition of ubiquitylated nucleosomes.

165 is study, we show that a strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3

166 When replication terminates, CMG is ubiquitylated on its Mcm7 subunit and disassembled by th

167 domain (CTD) of PTCH1 interacts with and is ubiquitylated on K1413 by the E3 ubiquitin-protein ligas

168 Furthermore, we show that USP4 is ubiquitylated on multiple sites including those on cyste

169 HMGCS1 is ubiquitylated on two C-terminal lysines during mTORC1 in

170 und at high affinity and is released only in ubiquitylated or partially disassembled forms.

171 lysine residues overlap with those that are ubiquitylated, our results suggest that one major functi

172 cleolar isolation revealed a distribution of ubiquitylated p53 that supports these findings.

173 This generates the apoptotically active non-ubiquitylated p53.

174 n incoming nucleotide, and wild type or mono-ubiquitylated PCNA (Ub-PCNA).

175 c finger (UBZ) domain, helps recruit FAN1 to ubiquitylated PCNA accumulated at stalled forks.

176 rtan, a protein that specifically recognizes ubiquitylated PCNA and plays an important role in cellul

177 We observed increased levels of ubiquitylated PCNA and significantly lower mutation freq

178 ing FAN1 activities and its interaction with ubiquitylated PCNA may offer therapeutic opportunities f

179 Directed against total ubiquitylated PCNA or specifically K63-polyubiquitylated

180 s1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambiv

181 In vitro, Spartan engages ubiquitylated PCNA via both a PIP box and a UBZ domain.

182 Surprisingly, while Spartan is recruited by ubiquitylated PCNA, knockdown of Spartan compromised chr

183 Thus, as a "reader" of ubiquitylated PCNA, Spartan promotes an unexpected feed-

184 regulating peroxisome abundance by removing ubiquitylated peroxisome membrane proteins to prevent pe

185 pla3(148M/M) mice, and only trace amounts of ubiquitylated PNPLA3 were seen in these animals.

186 ckdown of USP47 causes an increased level of ubiquitylated Pol beta, decreased levels of Pol beta, an

187 and cisplatin indicated that the undegraded ubiquitylated polymerase was mostly unbound or only loos

188 proteasomes by concurrent interactions with ubiquitylated proteasome subunits/targets and lipidated

189 by an absence of eif2alpha phosphorylation, ubiquitylated protein accumulation, and proteotoxicity.

190 ceptor plays a prominent role in recognizing ubiquitylated protein aggregates for vacuolar degradatio

191 ) and was associated with elevated levels of ubiquitylated protein and stabilization of the Ub-depend

192 ESCRT-I complex, which functions in sorting ubiquitylated protein cargoes into multivesicular bodies

193 ovide a general model for the disassembly of ubiquitylated protein complexes by Cdc48.

194 on degradation substrate exhibited decreased ubiquitylated protein degradation.

195 SIL1 function in mouse results in ER stress, ubiquitylated protein inclusions, and degeneration of sp

196 o tightly regulated and determines whether a ubiquitylated protein is destroyed or deubiquitylated an

197 red, which could suggest that it cleaves the ubiquitylated protein itself.

198 ata 1) demonstrate that the enhanced pool of ubiquitylated protein observed during neuritogenesis in

199 roteins play an important role in delivering ubiquitylated protein substrates to the proteasome for d

200 Through mass spectrometric analysis of ubiquitylated proteins affinity-purified from etiolated

201 n vitro degradation of ubiquitylated and non-ubiquitylated proteins and did not display substrate-sti

202 gnaling (Sts)-2 is associated with c-Cbl and ubiquitylated proteins and has been implicated in the re

203 Moreover, JS-K decreases total ubiquitylated proteins and increases p53 levels, which i

204 iquitylated proteins or co-isolation of both ubiquitylated proteins and their interacting proteins.

205 Ubiquitylated proteins are degraded by the 26 S protease

206 Ubiquitylated proteins are directed into a large number

207 It has been suggested that ubiquitylated proteins are directed to inclusion bodies

208 Ubiquitylated proteins are typically recognized using on

209 rotein sorting (VPS) pathway, where it binds ubiquitylated proteins as they are sorted into vesicles

210 tein recognition domain that is used to bind ubiquitylated proteins during vacuolar protein sorting,

211 aproteasomal ubiquitin receptor that targets ubiquitylated proteins for degradation.

212 andem ubiquitin-binding domains that protect ubiquitylated proteins from degradation.

213 n ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the p

214 Analysis of ubiquitylated proteins identified by di-glycine remnant

215 receptors Ufd1-Npl4 are essential to unfold ubiquitylated proteins in many areas of eukaryotic cell

216 Interestingly, neither of these two ubiquitylated proteins interacts with Nedd4 through PY-m

217 in of Vps36/ESCRT-II also inhibit sorting of ubiquitylated proteins into the yeast vacuole.

218 Intracellular deposition of aggregated and ubiquitylated proteins is a prominent cytopathological f

219 The ability to bind and cleave ubiquitylated proteins is consistent with AT3 playing a

220 a dynamic balance between free ubiquitin and ubiquitylated proteins is key to UPS function, the mecha

221 whereas the decision to degrade or sequester ubiquitylated proteins is mediated by the Hsp70s.

222 Proteasomal degradation of ubiquitylated proteins is tightly regulated at multiple

223 These effects resulted in an accumulation of ubiquitylated proteins leading to protein aggregation an

224 either selective purification of covalently ubiquitylated proteins or co-isolation of both ubiquityl

225 TUG depletion had no marked effect on total ubiquitylated proteins or p97 localization, TUG overexpr

226 proteins, yet the cellular role and fate of ubiquitylated proteins remain unclear.

227 Short-lived ubiquitylated proteins that are normally rapidly degrade

228 bition and accumulate high-molecular weight, ubiquitylated proteins that are poorly degraded by the p

229 It binds ubiquitylated proteins through its Ubiquitin Interacting

230 ut the mechanisms that govern recruitment of ubiquitylated proteins to IBs are not well understood.

231 ociated (UBL/UBA) proteins that help shuttle ubiquitylated proteins to the 26S proteasome for breakdo

232 ors that aid in the handling and delivery of ubiquitylated proteins to the proteasome.

233 nickel chelate-affinity chromatography, the ubiquitylated proteins were trypsinized, separated by tw

234 asome inhibitors, which block degradation of ubiquitylated proteins, are widely used in research, and

235 chains, which are detectable on endogenously ubiquitylated proteins, dampen cellular turnover of thes

236 ssue-dependent reductions of diubiquitin and ubiquitylated proteins, increased deubiquitylation activ

237 to the licensing factor CDT-1 and additional ubiquitylated proteins, thus promoting CDC-48/p97-depend

238 glycine remnant profiling was used to reveal ubiquitylated proteins, which in combination with whole-

239 interaction of the endocytic machinery with ubiquitylated proteins.

240 interaction of VCP with Ufd1 as well as with ubiquitylated proteins.

241 at has a critical role in the degradation of ubiquitylated proteins.

242 s been spent on methods to purify and detect ubiquitylated proteins.

243 ted in accumulation of high molecular weight ubiquitylated proteins.

244 n, negatively regulates cellular turnover of ubiquitylated proteins.

245 ss1 cause growth defects and accumulation of ubiquitylated proteins.

246 ectrophoresis to identify phosphorylated and ubiquitylated receptor intermediates.

247 Required for Transport (ESCRT), which target ubiquitylated receptors to intra-lumenal vesicles (ILVs)

248 These misfolded channels are poly-ubiquitylated, recognized by p97, and targeted for prote

249 itro, and ubp3 cells have elevated levels of ubiquitylated RNAPII in vivo.

250 In agreement with this, ubiquitylated RNAPII is hypophosphorylated at serine 5 i

251 ated T cells and stabilized UBR5, which then ubiquitylated RORgammat in response to TGF-beta signalli

252 ed ubiquitin ligase activity, and similarly, ubiquitylated Siz1p demonstrates reduced SUMO ligase act

253 Due to the low abundance of ubiquitylated species in biological samples, considerabl

254 recognition protein Rad4 and an increase in ubiquitylated species of Rad23.

255 Ubiquitylated species of the transducin gamma-subunit (T

256 d-light treatment, PhyA becomes the dominant ubiquitylated species, with ubiquitin attachment sites m

257 ic target proteins by modulating the pool of ubiquitylated species.

258 chain, and provide evidence that it and the ubiquitylated substrate are committed to each other afte

259 The resulting multi-ubiquitylated substrate is degraded by the 26S proteasom

260 The identity of the ubiquitylated substrate on bacteria is unknown.

261 ad4, a Rad23-binding protein, also regulates ubiquitylated substrate turnover.

262 -terminal Arg of a substrate and target the (ubiquitylated) substrate to the proteasome.

263 en shown to bind ubiquitin (Ub) chains, many ubiquitylated substrates also associate with 26S proteas

264 family of proteins that preferentially binds ubiquitylated substrates and multi-Ub chains through a m

265 TUG overexpression caused an accumulation of ubiquitylated substrates and targeted both TUG and p97 t

266 Remarkably, in Ssa1/Ssa2-depleted cells, ubiquitylated substrates are sequestered into detergent-

267 Proteasomes are activated when ubiquitylated substrates bind to them and interact with

268 lar machinery responsible for recognition of ubiquitylated substrates can make subtle distinctions be

269 19S regulatory particle (RP), which prepares ubiquitylated substrates for degradation, has remained e

270 uggesting a possible role in the transfer of ubiquitylated substrates from Cdc48 to the proteasome or

271 large network of adaptor proteins to process ubiquitylated substrates in different cellular pathways,

272 proximity labeling method that biotinylates ubiquitylated substrates in proximity to an E3 ligase of

273 in recent years to comprehensively identify ubiquitylated substrates of a particular pathway.

274 mitochondria from mouse neurons to identify ubiquitylated substrates of endogenous Parkin.

275 roach, we inhibited the proteasome to "trap" ubiquitylated substrates on the SCF(FBXW11) E3 complex.

276 ubiquitylation, unfolding, and channeling of ubiquitylated substrates to the 20S proteasome.

277 ting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their deg

278 The mechanism underlying the delivery of ubiquitylated substrates to the proteasome is poorly und

279 nt domains, Rad23 serves as a bridge linking ubiquitylated substrates to the proteasome.

280 binding protein involved in the transfer of ubiquitylated substrates to the proteasome.

281 MultiDsk binds ubiquitylated substrates with unprecedented avidity, and

282 SP29 displayed nonspecific reactivity toward ubiquitylated substrates.

283 quence of events prior to the degradation of ubiquitylated substrates.

284 mechanisms used by the proteasome to capture ubiquitylated substrates.

285 uitin conjugates directly and so link p97 to ubiquitylated substrates.

286 tasis that plays a central role in unfolding ubiquitylated substrates.

287 ever, the accumulated p68 appears to be poly-ubiquitylated, suggesting a possible defect in proteasom

288 E-STUB accurately identifies the direct ubiquitylated targets of protein degraders, including co

289 trategies have emerged to isolate endogenous ubiquitylated targets, including technologies based on t

290 sin-containing protein (VCP) is recruited to ubiquitylated Tau fibrils, resulting in their efficient

291 UBE3A ubiquitylated the highly conserved Lys160 residue on SIR

292 Basally, ubiquitylated translocon import substrates accumulate, s

293 K991 becomes ubiquitylated upon oxidative DNA damage, and while CSB K

294 These phosphorylation events allow ubiquitylated Vac17 to be released from Myo2 and Vac8.

295 We demonstrate that Miranda is ubiquitylated via its C-terminal domain; removal of this

296 Because DNA ligase I has been reported to be ubiquitylated, we used a proteomic approach to map ubiqu

297 In addition, Tgamma was exclusively ubiquitylated when Tbetagamma was dissociated from Talph

298 gene expression unless the activator is mono-ubiquitylated, which protects it from the ATPases.

299 Because few ubiquitylated yeast proteins are known to be degraded by

300 We report here that a subpopulation of ubiquitylated yeast SOD1 and other ubiquitylated mammali