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1 oluble Htt-polyQ aggregates using analytical ultracentrifugation.
2 by solution NMR spectroscopy and analytical ultracentrifugation.
3 Released EVs were isolated by differential ultracentrifugation.
4 subjects or patients with CKD by sequential ultracentrifugation.
5 ical properties following iodixanol gradient ultracentrifugation.
6 ects, as well as mouse HDL, were isolated by ultracentrifugation.
7 in solution, as revealed through analytical ultracentrifugation.
8 ddition of sI-Pht, as assessed by analytical ultracentrifugation.
9 AA80, and further confirm this by analytical ultracentrifugation.
10 2 +/- 10 mol/mol as determined by analytical ultracentrifugation.
11 multi-angle light scattering, and analytical ultracentrifugation.
12 ro study using gel filtration and analytical ultracentrifugation.
13 ion of hydrodynamic parameters in analytical ultracentrifugation.
14 ules using sedimentation velocity analytical ultracentrifugation.
15 on chromatography and equilibrium analytical ultracentrifugation.
16 ing and stochiometric analysis by analytical ultracentrifugation.
17 he CC and the NTD as monitored by analytical ultracentrifugation.
18 ter purification by sucrose density gradient ultracentrifugation.
19 ) ( approximately 10(6) IU/ml), even without ultracentrifugation.
20 at can be purified by immunoprecipitation or ultracentrifugation.
21 re sacrificed and plasma MP were isolated by ultracentrifugation.
22 size exclusion chromatography and analytical ultracentrifugation.
23 confirmed to exist in solution by analytical ultracentrifugation.
24 wn by fluorescence anisotropy and analytical ultracentrifugation.
25 ysis of supernatants and pellets obtained by ultracentrifugation.
26 nsmission electron microscopy and analytical ultracentrifugation.
27 butions to homodimer stability by analytical ultracentrifugation.
28 e prepared by sequential ultrafiltration and ultracentrifugation.
29 ious chromatography resins and by analytical ultracentrifugation.
30 pendent proteins), based on density gradient ultracentrifugation.
31 e-detected sedimentation velocity analytical ultracentrifugation.
32 those from solution studies using analytical ultracentrifugation.
33 cell-culture supernatant fluids by isopycnic ultracentrifugation.
34 dimentation-diffusion analysis by analytical ultracentrifugation.
35 nal hydrodynamic information from analytical ultracentrifugation.
36 tions, as analyzed by sedimentation velocity ultracentrifugation.
37 lation Kit or by differential centrifugation/ultracentrifugation.
38 Exosomes were prepared using ultracentrifugation.
39 ligonucleotide-binding assays and analytical ultracentrifugation.
40 (1) analysis of Lp(a) fractions isolated by ultracentrifugation; (2) immunoprecipitation of plasma u
44 angle X-ray scattering (SAXS) and analytical ultracentrifugation analysis of the EcoP15I holoenzyme a
46 onal cryo-electron microscopy and analytical ultracentrifugation analysis, we determined the structur
48 uman cytotrophoblasts (CTBs) by differential ultracentrifugation and characterized them using transmi
54 d several domain constructs using analytical ultracentrifugation and correlated VAI binding and PKR i
56 arance with and without exosomes isolated by ultracentrifugation and determined exosome-induced amylo
58 Mutational analysis coupled with analytical ultracentrifugation and equilibrium denaturations showed
61 rat brain alphabeta-tubulin using analytical ultracentrifugation and fluorescence anisotropy, observi
63 TP protofilaments was observed by analytical ultracentrifugation and fluorescence correlation spectro
64 293T cell EVs isolated by flotation gradient ultracentrifugation and from exosomes containing the tet
67 m purified human platelets, were isolated by ultracentrifugation and labeled with biotin or PKH67.
69 of our OmpW-micelle complex using analytical ultracentrifugation and molecular dynamics simulations.
71 inker with sedimentation velocity analytical ultracentrifugation and NMR spectroscopy reveals that th
73 m spectra, as well as equilibrium analytical ultracentrifugation and size exclusion chromatography.
75 e of truncated M. sexta sGC using analytical ultracentrifugation and small-angle X-ray scattering (SA
77 ution structure of native FHR5 by analytical ultracentrifugation and small-angle X-ray scattering.
79 lar lysate fractionation by density gradient ultracentrifugation and subsequent analysis by proteome-
80 20 and 400 nm from human serum and FBS using ultracentrifugation and sucrose gradient centrifugation.
82 tions in relation to C3b and C3u, analytical ultracentrifugation and x-ray and neutron scattering stu
83 nd temperatures was determined by analytical ultracentrifugation and X-ray and neutron scattering.
85 pattern of multimers observed in analytical ultracentrifugation, and a concentration dependence of a
86 size-exclusion chromatography and analytical ultracentrifugation, and also binds to its putative rece
87 tein/lipid ratio shown by isopycnic gradient ultracentrifugation, and are small in size with a mean d
88 gle particle electron microscopy, analytical ultracentrifugation, and bio-layer interferometry, we pr
90 amagnetic relaxation enhancement, analytical ultracentrifugation, and DEER EPR, indicate that the tra
91 ing small-angle x-ray scattering, analytical ultracentrifugation, and dynamic light scattering techni
92 tallography, solution scattering, analytical ultracentrifugation, and electron microscopy we determin
93 d to multiangle light scattering, analytical ultracentrifugation, and electron paramagnetic resonance
94 oblot, immunoprecipitation, density gradient ultracentrifugation, and enzyme-linked immunosorbent ass
96 tures, Ca(2+) binding capacities, analytical ultracentrifugation, and light-scattering profiles of th
97 ntified by NMR spectroscopy, SPR, analytical ultracentrifugation, and microcalorimetry glycopeptides
98 NMR chemical shift perturbation, analytical ultracentrifugation, and native electrospray ionization
99 entary surface plasmon resonance, analytical ultracentrifugation, and native mass spectrometry experi
100 tical gel filtration, sedimentation-velocity ultracentrifugation, and negative-stain electron microsc
101 egates and Abeta monomers using differential ultracentrifugation, and purifying them >6000 fold by du
102 sm and fluorescence spectroscopy, analytical ultracentrifugation, and semi-native gel electrophoresis
103 we used native mass spectrometry, analytical ultracentrifugation, and size-exclusion chromatography c
104 using microscale thermophoresis, analytical ultracentrifugation, and size-exclusion chromatography s
105 tering, dynamic light scattering, analytical ultracentrifugation, and small angle X-ray scattering ea
106 graphy, dynamic light scattering, analytical ultracentrifugation, and small angle x-ray scattering ex
108 easured after vertical spin density-gradient ultracentrifugation, and triglycerides were directly mea
109 higher sedimentation velocities in gradient ultracentrifugations, and a longer incubation time in an
111 ent, sonication and sucrose density-gradient ultracentrifugation are subsequently used to isolate the
116 id testing by vertical spin density gradient ultracentrifugation (Atherotech, Birmingham, Alabama) fr
117 profiling by vertical spin density gradient ultracentrifugation (Atherotech, Birmingham, Alabama) fr
118 Here, we show how two techniques, analytical ultracentrifugation (AUC) and total organic carbon analy
119 ces in instrumentation have moved analytical ultracentrifugation (AUC) closer to a possible validatio
122 Sedimentation equilibrium (SE) analytical ultracentrifugation (AUC) is a gold standard for the rig
125 ission Electron Microscopy (TEM), Analytical Ultracentrifugation (AUC), and UV/Vis spectroscopy.
127 e report the novel application of Analytical Ultracentrifugation (AUCF) to characterise the polymeric
128 on agrees quantitatively with our analytical ultracentrifugation-based measurements and previously pu
129 01Bio, Wako and iZON along with conventional ultracentrifugation-based method for exosome yield, puri
131 ne has shown that isopycnic density gradient ultracentrifugation can produce structurally and electro
132 ochron iButton) that can be inserted into an ultracentrifugation cell assembly and spun at low rotor
133 ing small-angle x-ray scattering, analytical ultracentrifugation, circular dichroism, x-ray fiber dif
139 mers to a single compact dimer by analytical ultracentrifugation, coupled with a >30 A decrease in ma
140 tive polyacrylamide gel electrophoresis, and ultracentrifugation data show that the dimer is in equil
142 this protocol describes sample preparation, ultracentrifugation, data acquisition, and data analysis
144 The recent application of density gradient ultracentrifugation (DGU) for structural sorting of sing
145 ation of (13)C labeling and density gradient ultracentrifugation (DGU) to produce an array of (13)C-l
146 m estuarine sediments using density gradient ultracentrifugation (DGU), followed by online flow-throu
149 biophysical approaches, including analytical ultracentrifugation, dynamic light scattering, and therm
150 nts (FtsZ-GTP) as demonstrated by analytical ultracentrifugation, dynamic light scattering, fluoresce
151 ns below 30 muM, as determined by analytical ultracentrifugation, dynamic light scattering, size excl
152 d as a tetramer, as determined by analytical ultracentrifugation, electron microscopy, and electrospr
153 methods that includes sedimentation velocity ultracentrifugation, electron microscopy, and hydrodynam
158 were synthesized and utilized in analytical ultracentrifugation experiments and demonstrate that MER
159 e bonds, NMR solution studies and analytical ultracentrifugation experiments are reported in addition
160 X-ray co-crystal structure analysis and ultracentrifugation experiments clearly demonstrate that
164 thermal titration calorimetry and analytical ultracentrifugation experiments indicated that the preva
167 synchrotron x-ray scattering and analytical ultracentrifugation experiments showed that the carbohyd
168 enceforth referred to as VanS) in analytical ultracentrifugation experiments to study VanS oligomeric
174 ptor (hPVR) using various techniques such as ultracentrifugation, fluorescence-activated cell sorting
175 icle and dissolved Ag fractions (ICPMS after ultracentrifugation), for the characterization of Ag-NP
177 pplied to RNA extracted from EVs isolated by ultracentrifugation from the plasma of five healthy volu
178 er related methods (filter assay, analytical ultracentrifugation, gel electrophoresis and size-exclus
180 ce of the sample cell assembly in analytical ultracentrifugation holds the sample solution between wi
182 the proteins was determined using analytical ultracentrifugation in the absence or presence of high c
183 them (>70%) are retained even after extended ultracentrifugation in the preparations of vesicle-deple
185 ues (dynamic light scattering and analytical ultracentrifugation) in an attempt to provide the basis
186 ed on sedimentation velocity (SV) analytical ultracentrifugation, in combination with a novel multiwa
188 mutagenesis, gel filtration, and analytical ultracentrifugation indicated that dimers form the basic
193 d using SWCNTs separated by density gradient ultracentrifugation, it is found that the high-pressure
194 th placebo, evolocumab significantly reduced ultracentrifugation LDL cholesterol at 12 weeks by 30.9%
195 he primary endpoint was percentage change in ultracentrifugation LDL cholesterol from baseline at wee
196 12, LDL-C reduction measured by preparative ultracentrifugation (least squares mean [standard error
197 cal lipids were also analyzed using gradient ultracentrifugation, liquid chromatography-mass spectrom
198 oteins by Isotope Tagging after Differential ultraCentrifugation (LOPIT-DC) and compare this method t
202 solated through an optimized ultrafiltration/ultracentrifugation method and characterized with variou
205 by size exclusion chromatography, analytical ultracentrifugation, multiangle laser light scattering,
206 and software for multiwavelength analytical ultracentrifugation (MWL-AUC) experiments, demonstrating
207 s verified using a combination of analytical ultracentrifugation, native electrospray mass spectromet
208 termediates were characterized by analytical ultracentrifugation, native gel electrophoresis, and ele
209 amic and structural studies using analytical ultracentrifugation, NMR, and small-angle x-ray scatteri
211 c assembly which are confirmed by analytical ultracentrifugation of both the crystallized domain and
213 l packing analysis, combined with analytical ultracentrifugation of Sonic Hh-GAG complexes, suggests
215 Dynamic light scattering and analytical ultracentrifugation on UNC-6 (with and without its C-dom
217 the need for less efficient methods such as ultracentrifugation or high-performance liquid chromatog
218 substrate for conversion, and attenuated by ultracentrifugation or incubation with SOD1 misfolding-s
219 ired purification of viral particles through ultracentrifugation or PEG precipitation, was PEG indepe
221 ority of studies to date have focused on the ultracentrifugation pellet, potentially losing a novel s
225 ix-free assay of co-sedimentation analytical ultracentrifugation, reinforced by dynamic light scatter
226 xclusion chromatography (SEC) and analytical ultracentrifugation, remain the default tools in charact
227 aining fraction could be however purified by ultracentrifugation resulting in the mitigation of MCPDE
228 small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in
229 rified complexes by sucrose density gradient ultracentrifugation revealed that the three proteins for
230 lectron microscopy with data from analytical ultracentrifugation reveals the crucial role of kinetic
231 assays, PARP activity assays, and analytical ultracentrifugation sedimentation analysis, we found tha
233 from both GST pulldown assays and analytical ultracentrifugation show that GstDnaB1-300 is sufficient
234 fluorescence anisotropy decay and analytical ultracentrifugation show that the iM structure has a com
239 nce (NMR) spectroscopy as well as analytical ultracentrifugation, showing that they have a weight ave
241 Hsp90 dimers and oligomers were evaluated by ultracentrifugation, size-exclusion chromatography coupl
242 eltaC) is further validated using analytical ultracentrifugation, size-exclusion chromatography, NMR
245 ng size-exclusion chromatography, analytical ultracentrifugation, small-angle X-ray scattering (SAXS)
246 ses including circular dichroism, analytical ultracentrifugation, small-angle x-ray scattering, and h
247 magnetic resonance spectroscopy, analytical ultracentrifugation, small-angle X-ray scattering, molec
248 and fluorescence spectroscopies, analytical ultracentrifugation, splicing assays, and cell microscop
251 ross-linking, gel filtration, and analytical ultracentrifugation studies aimed at evaluating interact
252 ecSecA dimerization based on our analytical ultracentrifugation studies of SecA L6A and shown to for
255 orescence, circular dichroism and analytical ultracentrifugation studies showed that POT1 binding is
257 ng pull-down, gel filtration, and analytical ultracentrifugation studies, we show that the N-terminus
258 -dependent circular dichroism and analytical ultracentrifugation suggest that the htt(NT) sequence, w
259 h extracellular vesicles (EVs) and remain in ultracentrifugation supernatants of cell-conditioned med
260 ination of X-ray crystallography, analytical ultracentrifugation, surface plasmon resonance and doubl
261 oscopy and sedimentation velocity analytical ultracentrifugation (svAUC) to undertake initial structu
262 sorting method can be combined with existing ultracentrifugation SWCNT sorting methods to produce "or
263 this work, using a combination of analytical ultracentrifugation techniques and DNA binding experimen
264 , to show by NMR spectroscopy and analytical ultracentrifugation that at biologically relevant concen
265 by surface plasmon resonance and analytical ultracentrifugation, that individual HTH motifs of the B
266 Liposome suspensions were concentrated by ultracentrifugation; the pellets were reconstituted in w
267 ld-type proteins were assessed by analytical ultracentrifugation, thioflavin T binding, transmission
270 of site-directed mutagenesis and analytical ultracentrifugation to derive thermodynamic parameters f
273 interactions in solution, we used analytical ultracentrifugation to measure the dimerization constant
274 nia We used fluorescence-detected analytical ultracentrifugation to measure tubulin dissociation over
275 spectrometry, was complemented with density ultracentrifugation to reveal the colocalized, or dissoc
277 ng size exclusion chromatography, analytical ultracentrifugation, transmission electron, and atomic f
278 ENP isolation from the conditioned medium by ultracentrifugation (UC) can take ~3 d, the AF4 fraction
280 EVs have traditionally been purified by ultracentrifugation (UC), however UC has limitations, in
281 gation behavior of AgNPs were examined using ultracentrifugation, ultrafiltration, and asymmetrical f
282 anotubes (SWNTs), sorted by density-gradient ultracentrifugation, undergo self-assembly using depleti
287 rential scanning calorimetry, and analytical ultracentrifugation, we demonstrate the AgamOBP48 dimeri
289 gel filtration chromatography and analytical ultracentrifugation, we found that a fully functional co
290 small-angle X-ray scattering and analytical ultracentrifugation, we found that ClipCG12 adopts a lar
292 gle x-ray scattering analysis and analytical ultracentrifugation, we show that NaD1 forms dimers in s
293 ry human DC-secreted EV (DC-EV), isolated by ultracentrifugation, were characterized for their size,
294 size-exclusion chromatography and analytical ultracentrifugation, whereas other class III nucleotidyl
295 aracterization technique based on analytical ultracentrifugation, which demonstrates exceptional pote
298 al cell fractionation using density gradient ultracentrifugation with multiplexed quantitative proteo
300 ion structure by a combination of analytical ultracentrifugation, X-ray scattering and constrained mo