ライフサイエンスコーパス: unwound

1 hile hydrolyzing approximately 5 ATPs per bp unwound.

2 telomeric duplexes that are otherwise poorly unwound.

3 to the substrates that were the most readily unwound.

4 tly more than one ATP molecule per base pair unwound.

5 es in the 5' untranslated region of mRNA are unwound.

6 hese structures, the SH1 helix is seen to be unwound.

7 icates that the U5-IR stem becomes partially unwound.

8 in initial rate and maximum amount of duplex unwound.

9 probe-plasmid interactions once the site is unwound.

10 and nucleic acid that forms as duplexes are unwound.

11 as the double helix melts, and before it is unwound.

12 teraction of a Chi site contained within the unwound 3' ss-DNA tail with the RecC subunit during DNA

13 UvrD preferentially unwound 3'-single-stranded tailed duplex substrates over

14 wounded (277+/-15 microm) compared with the unwounded (356+/-6 microm) corneas (P=0.0008) after 16 w

15 in maintaining the split trajectories of the unwound 5' and 3' strands.

16 ing, whereby the AdnA nuclease processes the unwound 5' strand to liberate a short oligonucleotide pr

17 of the panhandle structure by N protein, the unwound 5' terminus likely remains transiently bound to

18 in the viral RNA and remains associated with unwound 5' terminus.

19 All four proteins unwound a 10 bp helix in vitro in the presence of ATP; h

20 WRN unwound a 3'-single-stranded (ss)DNA-tailed duplex subst

21 P143 unwound a 40-nucleotide primer in an ATP-dependent manne

22 y base pairs it has unwound, and once it has unwound a critical length, it reverses the unwinding rea

23 FANCJ and BLM synergistically unwound a DNA duplex substrate with sugar phosphate back

24 However, Pif1 working with pol delta readily unwound a full-length Okazaki fragment initiated by a fo

25 blocking Rac1 interactions until irradiation unwound a helix linking LOV to Rac1.

26 In unwounded aged skin (versus unwounded younger skin), the

27 sodium-coupled transporter, LeuT, define an unwound alpha-helix as the central element of the ion-bi

28 isoforms and similar cleavage efficiency of unwound alpha1(I) and alpha2(I) chains suggested increas

29 ure would need to adopt an unusual, slightly unwound, alpha11/3 helix conformation (three complete tu

30 Furthermore, the maximum amount of duplex unwound also decreased with increasing stability.

31 a checkpoint response only after the DNA was unwound and DNA polymerase alpha had been loaded.

32 hyrin bound, the pi helix is not extended or unwound and is in the "substrate-bound" conformation.

33 ent stalling, G-quadruplexes are efficiently unwound and replicated.

34 ery to access the DNA, the chromatin must be unwound and the DNA cleared of histone proteins.

35 mation, the two terminal stems are "open" or unwound and the other stems are closed.

36 origins are recognized, the DNA molecule is unwound and the replicative helicase is loaded onto the

37 rnover conditions, although the substrate is unwound and the repressor displaced when the single-stra

38 bubble expands downstream until 18 bases are unwound and the RNA is at least 7 nt long, at which poin

39 e further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an a

40 After splicing, U2/U6 is unwound and U6 annealed to U4 to reassemble the tri-snRN

41 For initial nicking of the DNA, a locally unwound and unpaired DNA duplex forms a zipper via alter

42 for the monocyte-specific antigen CD11b, in unwounded and epithelial scrape-wounded mouse corneas.

43 NK, M-CSF, and OPG proteins were detected in unwounded and wounded mouse corneas by immunocytochemist

44 BLM can 'measure' how many base pairs it has unwound, and once it has unwound a critical length, it r

45 stalled forks are actively dechromatinized, unwound, and repressed by an ATR-dependent checkpoint pa

46 y and characteristically interacted with the unwound, antisense strand E7 siRNA.

47 No MCM2-positive staining was observed in unwounded areas at any time point.

48 67, whereas no Ki67 staining was observed in unwounded areas under any condition tested.

49 hat the DNA of the rDNA ARS is not as easily unwound as the H4 ARS.

50 te in heterotrimeric collagen I is partially unwound at equilibrium.

51 The alpha2 helix is unwound at its N terminus, which appears to be essential

52 uitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 4

53 ning the present tetrahydroepoxide adduct is unwound at the lesion site, whereas the diol epoxide add

54 e triple helical structure has to be locally unwound before hydrolysis, but this process is not well

55 , defined as the average number of basepairs unwound between two successive rate limiting steps in th

56 defined as the average number of base-pairs unwound between two successive rate-limiting steps repea

57 , with as many as 42,300 base pairs of dsDNA unwound by a single RecBCD enzyme molecule.

58 the tRNA acceptor stem is not substantially unwound by NC in the absence of the RNA genome, that is,

59 A PNA-RNA substrate was not unwound by NS3 under similar conditions.

60 The PNA-DNA substrate was unwound by NS3, but the observed rate of strand separati

61 rminated with 3'-ssDNA; however, such DNA is unwound by RecQ to create ssDNA for RecJ exonuclease.

62 3' single-stranded tail is not recognized or unwound by Sgs1.

63 s were functional RNAs (ribozymes) that were unwound by the helicase, and the first synthesised prote

64 a long forked duplex that is not completely unwound by the helicase.

65 After the duplex DNA template is unwound by the T7 helicase, specific primers anneal to t

66 eplaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8 complex.

67 are well separated from the promoter region unwound by the XPB DNA helicase and extend, respectively

68 an half of the mitochondrial genome could be unwound by Twinkle during a single DNA-binding event.

69 The interbend DNA was unwound by use of the intercalator chloroquine, or, alte

70 e rate of formation and the stability of the unwound complex depend profoundly on supercoiling and th

71 ty DnaA binding sites to a completely loaded unwound complex, increasing both the precision of DNA re

72 g by trapping the non-template strand in the unwound conformation.

73 it is typically assembled, and an extended, unwound conformation.

74 hat all three DNA strands adopt extended and unwound conformations similar to those of RecA-bound dsD

75 e site of VKOR that alters between wound and unwound conformations.

76 Unwounded contralateral eyes served as controls.

77 Contralateral eyes were unwounded controls.

78 In normal unwounded cornea, lumican is expressed by stromal kerato

79 helial injury, but not in keratocytes in the unwounded cornea.

80 < 0.01) fewer gammadelta T cells resident in unwounded corneal epithelium, which failed to increase i

81 Unwounded corneas served as control specimens.

82 KL, M-CSF, and OPG proteins were detected in unwounded corneas, but were expressed at higher levels i

83 In unwounded corneas, EGFR was localized in basal cells and

84 In unwounded corneas, TbetaR-I and TbetaR-II were present a

85 ansfer experiments (from wounded cultures to unwounded cultures) confirmed the existence of a soluble

86 to a novel conformation, that of a slightly unwound, curved, planar amphipathic alpha 11/3 helix (th

87 However, the structure of a pre-unwound D1D2:dsRNA complex remains elusive, and thus, th

88 a, including structures of apo-D1D2 and post-unwound D1D2:single-stranded RNA complex, and the struct

89 At high pH helix B is unwound, destroying the substrate binding pocket.

90 Local zones of easily unwound DNA are characteristic of prokaryotic and eukary

91 Although any individual RecBCD molecule unwound DNA at a constant rate for an average of approxi

92 g H. pylori addA(NUC)B or addAB(NUC) mutants unwound DNA but had approximately half of the exonucleas

93 the time-course for formation of completely unwound DNA displayed a distinct lag phase that increase

94 varying size, we demonstrate that all of the unwound DNA generated at a stalled replication fork can

95 ions affect the generation of a distorted or unwound DNA intermediate that has been implicated in hai

96 lecting the transient formation of partially unwound DNA intermediates during unwinding catalyzed by

97 lecting the transient formation of partially unwound DNA intermediates.

98 at very low ionic strength, where regions of unwound DNA may exist in the duplex.

99 s(-1) and a dissociation step from partially unwound DNA of k(off) = 1.9 s(-1).

100 but only if the R loops are within an easily unwound DNA sequence.

101 , MTERF1 binds a significantly distorted and unwound DNA structure, exhibiting a protein conformation

102 pin, slipped strand, triplex, quadruplex, or unwound DNA structures.

103 this region of T antigen provides the proper unwound DNA substrate for initiation to occur, we demons

104 The DnaB-DnaC complex binds to the unwound DNA within the Escherichia coli replication orig

105 ide (27), both of which also inhibited top2, unwound DNA, and are assumed to be DNA intercalators.

106 ular Cell, heterodimerizes with FANCM, binds unwound DNA, and reveals how the Fanconi anemia core com

107 a distinct lag phase for formation of fully unwound DNA, indicating that unwinding occurs in discret

108 that detect only the final product of fully unwound DNA.

109 s to oxidizable bases in the damaged, partly unwound DNA.

110 transiently in mRNA and in single-stranded, unwound DNA.

111 and stromal cells induces new HFs in adult, unwounded dorsal mouse skin.

112 d foot skin of patients with diabetes and in unwounded dorsal skin of diabetic mice (P < 0.05).

113 al ring-shaped T7 helicase molecules as they unwound double-stranded DNA (dsDNA) or translocated on s

114 DHX36 unwound dsDNA poorly compared with G4s of comparable int

115 ules are bound to each fork of the partially unwound dsDNA, and interact with the 5' arm and 3' ss/ds

116 vities that can operate on each strand of an unwound duplex DNA.

117 ested under conditions in which the helicase unwound duplex DNA.

118 Extension of the 5'-tail of the unwound duplex induces a large conformational change in

119 ucleotides to form a highly asymmetrical and unwound duplex.

120 ited to accommodate one or two strands of an unwound duplex.

121 o examine the fate of ORC when origin DNA is unwound during replication initiation, we determined the

122 vations suggest that most oriC copies become unwound during stationary phase, forming an initiation-l

123 DNA turn, consistent with the length of DNA unwound during transcription initiation.

124 m loop (U6 ISL), a stable helix that must be unwound during U4/U6 assembly.

125 c Holliday junction structures, which can be unwound efficiently by WRN and BLM.

126 Unwounded endothelium acted as a negative control.

127 expressed independent of the JNK pathway in unwounded epidermis.

128 ating epithelium, but rather in the adjacent unwounded epithelium of the cornea, with most cells bein

129 tructured loop or a Watson-Crick duplex were unwound equally well by both enzymes.

130 P-positive nerve density in both wounded and unwounded eyes compared with vehicle-treated corneas.

131 In unwounded eyes, cyclin D showed diffuse cytoplasmic loca

132 acunarity analysis effectively discriminated unwounded fibromodulin-null versus wild-type skin as wel

133 relate to the number of base pairs that are unwound for each ATP that is hydrolysed.

134 for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule.

135 e number of degranulated MCs is increased in unwounded forearm and foot skin of patients with diabete

136 many genetic observations, the detection of unwound fork structures in vivo and the identification o

137 gyrase-dependent formation of FI*, a highly unwound form of supercoiled DNA.

138 Cytosolic Ca(2+) concentrations in unwounded fou2 were found to be lower than in the unwoun

139 This strand is then unwound from its complement and transferred in the 5'-to

140 c stability are thus shown to be more easily unwound from one side than the other, in a quantifiable

141 These ultimately unwound further to reveal segmented portions of the fibe

142 ChlR1 unwound G-quadruplex (G4) DNA with a strong preference f

143 FANCJ unwound G4 DNA substrates in an ATPase-dependent manner.

144 metastructure is composed of bound water and unwound gelatin polypeptides.

145 mutants, harboring substitutions within the unwound GMG loop and substrate binding pocket that mimic

146 was in excess of the substrate, NS3 (500 nM) unwound >80% of a DNA substrate containing a 15-nucleoti

147 viously shown to bind to hormone as a partly unwound helix, forms a complete alpha-helix that displac

148 shed the virulence of DeltaBcsep4 mutants on unwounded hosts.

149 is reactions until about four base pairs are unwound in a burst.

150 FANCJ efficiently unwound in a kinetic and ATPase-dependent manner entropi

151 Short RNA helices are unwound in a single ATPase cycle, but the ATP requiremen

152 The number of base pairs unwound in a single binding event for Dda is increased f

153 helicase, i.e. the number of the base-pairs unwound in a single catalytic step is only 1.4(+/- 0.2).

154 r experiments, which show that SNAREs can be unwound in a single encounter with NSF.

155 blished an RNA substrate for NS3 that can be unwound in a single sub-step.

156 suggests that origin regions are frequently unwound in native chromatin.

157 U4 and U6 are then unwound in order for U6 to pair with U2 to form the spli

158 at 4-5 nt of downstream gap-proximal DNA are unwound in the binary complex.

159 eir complex with a bubble DNA having one arm unwound in the crystal.

160 amp opening allows DNA to be loaded into and unwound in the RNAP active-center cleft, that DNA loadin

161 mulated with a mechanism in which the DNA is unwound in two kinetic steps with rate constant of k(unw

162 als single strands soon after they have been unwound in vitro.

163 positions a nucleosome, evidently partially unwound, in a structure that facilitates Gal4 binding to

164 esence of excess enzyme, the quantity of DNA unwound increased significantly as the length of the sin

165 Strikingly, BLM unwound individual DNA molecules in a repetitive manner,

166 In turn, helix D is unwound into a flap now partially covering the N-termina

167 way, small interfering RNAs (siRNAs) must be unwound into their component strands, then assembled wit

168 The sequence unwound is the 18-bp A + T-rich segment that links the t

169 s manner while the second copies the already unwound lagging-strand template in a discontinuous manne

170 Consistently, WRN efficiently unwound large (CTG)(n) hairpins and promoted DNA polymer

171 to the central channel of the N-tier and the unwound leading single-strand DNA traverses the channel

172 esized both in wounded leaves and in distal, unwounded leaves in response to herbivory or other mecha

173 The increase in PLA activity in the systemic unwounded leaves was biphasic in wild-type tomato plants

174 aximizes at about 4-6 hr in both wounded and unwounded leaves, and then declines.

175 In unwounded leaves, the levels of these oxylipin-containin

176 G activity in extracts from both wounded and unwounded leaves.

177 Unwounded left eyes were normal controls.

178 structures were composed of chains that were unwound like a yarn ball.

179 While xDNA2 acts on ssDNA unwound mainly by the Xenopus Werner syndrome protein (x

180 Corneal nerve density was measured in unwounded mice.

181 age N4 gp2 protein, Drc likely binds locally unwound middle promoters and recruits the phage RNA poly

182 Electron microscopy revealed completely unwound molecules.

183 substrate containing a Holliday junction is unwound most efficiently.

184 In the unwounded mouse cornea, type XVIII collagen was expresse

185 D11b antigen were detected in the stromas of unwounded mouse corneas.

186 ing analysis of 6,154 cells from wounded and unwounded mouse skin revealed that MCS-01 primarily alte

187 standard substrate for 3'-->5' helicases, is unwound much less efficiently by BLM and WRN than are th

188 o form a block to SMARCAL1 or by reannealing unwound nascent strands to their parental template.

189 UT-1, PGK-1, nor VEGF mRNA was detectable in unwounded nontransgenic skin.

190 Although the existence of different unwound nucleosome states has been hypothesized, there h

191 the existence of two distinct states of the unwound nucleosome, which are accessible at physiologica

192 end of the triple helix, which may leave the unwound oligonucleotide susceptible to nuclease degradat

193 PcrA also efficiently unwound oligonucleotides containing a duplex region and

194 transmembrane segment 5 (TM5i) in either an unwound or a helical conformation.

195 These studies also suggest that partially unwound or noncomplementary regions of DNA could be phys

196 ng that the oxidation process is favored for unwound or single-strand regions of DNA.

197 e activity appears to be specific for highly unwound, or single strand-containing substrates.

198 The class D mutants unwound origin DNA normally but were compromised in thei

199 of RPA, the ssDNA binding protein that marks unwound origins before polymerase recruitment.

200 ppears to be dictated by the geometry of the unwound part of the transmembrane (TM) helix 3, mostly a

201 The NMDGT motif on the partially unwound part of the transmembrane helix TM7 and the resi

202 s release to the cytoplasm provided that the unwound part of TM3 switches from a shielding to a yield

203 To determine whether unwounded plants can release significant amounts of vir

204 ciently transferred this T-DNA into cells of unwounded plants in the absence of exogenous vir gene in

205 e growth restriction can be recapitulated in unwounded plants when the LOX3 pathway is activated gene

206 To activate LOX3-dependent JA production in unwounded plants, we employed hyperactive TPC1 variants.

207 ical interactions between A. tumefaciens and unwounded plants.

208 e and phospholipase), determine JA levels in unwounded plants.

209 trong effect on the initial concentration of unwound plasmids.

210 ites attention to the functional role of the unwound portion of TM helices (TM6 Trp-202-Glu-208 in Ad

211 athway between the sodium-binding sites, the unwound portion of transmembrane helix 1 and the substra

212 ound-induced (win) mRNAs are detected in the unwounded portion of that leaf and in specific leaves th

213 f residues in the opposing hairpin loops and unwound portions of adjacent helices.

214 branch migration by RecA, where a completely unwound product consisting of the paired nascent leading

215 d SSB; however, RuvAB generates a completely unwound product consisting of the paired nascent leading

216 tion fork and stimulates FEN-1 to cleave the unwound product in a structure-dependent manner.

217 rved pi helix was in the extended, partially unwound "product release" state.

218 ep, preventing the transition of a partially unwound promoter DNA intermediate to the fully opened DN

219 he ability of BC200 to act as an acceptor of unwound quadruplexes via a cytosine-rich region near the

220 oncentrations (1 nM), the bulk of the DNA is unwound rapidly by pre-bound UvrD complexes upon additio

221 A shorter 12-bp substrate is unwound rapidly under single turnover conditions.

222 Unwounded rat corneas served as control samples.

223 showed that heteroduplex DNA is likely to be unwound rather than degraded.

224 o acids 128-142 and 147-154) separated by an unwound region (amino acids 143-146).

225 xibility of a Gly-Met-Gly (GMG) motif in the unwound region of transmembrane segment 6 (TM6) is centr

226 of oriC, whereas DnaB fails to bind to this unwound region, the open structure is insufficient by it

227 C, likely due to the presence of extensively unwound regions in the plasmid.

228 All proteins unwound RNA and DNA best at pH 6.5, which demonstrate th

229 d translocate axially from wounded shoots to unwounded roots in a LOX2-dependent manner.

230 RNA, thereby facilitating the release of the unwound rRNA mother strand and the recycling of DbpA for

231 omain (TM6a) that is separated by a central, unwound section from a cytoplasmically localized domain

232 the reporter in the roots and hypocotyls of unwounded seedlings.

233 ing, duplex unwinding and degradation of the unwound sense strand and RNA-induced silencing complex f

234 The first base pair of the duplex is unwound, separating the 5' nucleotide of the guide from

235 cases, the complexes are characterized by an unwound SH1 helix first seen in an unusual 2.5-A scallop

236 ked its N-terminal RPA interaction site also unwound short DNA duplex substrates similar to wild-type

237 AdnAB helicase under conditions in which the unwound single strands are coated by SSB and thereby pre

238 ing a synthetic sequence that mimics freshly unwound single-stranded DNA at replication fork showed t

239 o load RecA protein onto the chi-containing, unwound single-stranded DNA.

240 lecular G4 DNA likely to form in transiently unwound single-stranded genomic regions.

241 ge (80S), ATP-enhanced complex that contains unwound siRNAs, cofractionates with known RNAi factors,

242 ression was strongly increased compared with unwounded skin and the signal was localized to the wound

243 tral endopeptidase expression in wounded and unwounded skin as well as in cells derived from human sk

244 sion, the presence of nondegranulated MCs in unwounded skin is required for proper wound healing, and

245 undetectable in keratinocytes isolated from unwounded skin, but induced in cells following contact w

246 In unwounded skin, cells are nourished by plasma.

247 In unwounded skin, human keratinocytes (HKs) are in contact

248 d skin, but not in ganglia that projected to unwounded skin, suggesting that neurons respond to a loc

249 during the anagen phase of the hair cycle in unwounded skin.

250 in dermatan sulfate synthesis compared with unwounded skin.

251 indistinguishable from those in age-matched unwounded skin.

252 g index (LI) that was markedly elevated from unwounded specimens in the first 48 h after abrasion.

253 ems primarily target transcripts, instead of unwound ssDNA in TECs, for immunity against double-stran

254 to defined DNA substrates and stabilizes the unwound ssDNA product, resulting in a ~5-fold stimulatio

255 nwinding and/or by POT1 loading on partially unwound ssDNA strands to prevent strand re-annealing.

256 vironment in the absence of the receptor, is unwound starting at T(32) to provide optimal contacts in

257 in complex with a 23-base pair dsRNA at pre-unwound state, revealing that two DDXs recognize a 2-tur

258 eported strain in the catalytically relevant unwound state, suggesting that this state is distinct fr

259 o capture its DNA substrate when it is in an unwound state.

260 A complex that is thought to represent a pre-unwound state.

261 might stabilize replication origins in their unwound state.

262 r results demonstrate not only that multiple unwound states exist but that their accessibility can be

263 We anticipate that the two unwound states reported here will be the basis for futur

264 nealing of a complementary RNA by making the unwound strand more accessible.

265 randed (ss) DNA that is complementary to the unwound strand.

266 ity of WRN helicase by maintaining partially unwound strands in a melted state, rather than preventin

267 e, a serine and a main-chain carbonyl in the unwound stretch of trans-membrane helix 5 at the deepest

268 conjunctiva exhibited increases in LIs from unwounded subjects at singular timepoints (within 24 h o

269 ion did not require new DNA synthesis on the unwound template strand but did require RNA primer synth

270 es with high affinity (Ka > 10(8) M(-1)) and unwound the DNA duplex through intercalation (unwinding

271 When bound to ssDNA, the enzyme unwound the DNA in the 3'-to-5' direction.

272 anded DNA-binding protein, FANCJ efficiently unwound the DNA substrate harboring the thymine glycol d

273 WRN efficiently unwound the forked duplex with streptavidin bound just u

274 ated that if the terminator DNA is partially unwound, the resulting melted DNA could bind tightly to

275 HrP helix is gently curved and C-terminally "unwound." The receptor accommodates the altered binding

276 -tail and the length of the duplex DNA to be unwound, this activity is sufficiently strong to mask th

277 under certain conditions RecG preferentially unwound three-strand junction DNA.

278 sform analysis in discriminating scar versus unwounded tissue in a wild-type mouse model.

279 In unwounded tissue, Smad 2 was cytoplasmic.

280 substrate binding by stabilizing the partly unwound TM1' helix.

281 ical barriers within the genome that must be unwound to ensure cellular genomic integrity.

282 differ when sequences are presented from an unwound triple helix versus an independent single strand

283 The two enzymes unwound triplexes without requirement for a duplex exten

284 ory cytokine TNFalpha is elevated >3-fold in unwounded TSG-6-null skin and increases further after wo

285 WRN unwound two important intermediates of replication/repai

286 ity on DNA polymers, showing that DNA can be unwound under extremely permissive conditions that favor

287 We find that U4/U6 is efficiently unwound using DNA oligonucleotides by coupling unwinding

288 he amount of double-stranded DNA that can be unwound using the free energy derived from hydrolysis of

289 ity and the number of intraepithelial DCs in unwounded (UW) corneas.

290 s frequent mode, longer stretches of DNA are unwound via a path that is separate from the one leading

291 However, the same duplex was readily unwound when a noncomplementary 5' tail was added to for

292 The 3' stem is unwound when U6 RNA base-pairs with U4 RNA during splice

293 Compared with the epithelial layer that is unwounded, which is non-migratory, solid-like and jammed

294 unwind the DUE region but unlike the easily unwound, wild-type H4 ARS.

295 Particularly, the GC-rich dsDNAs are unwound with lower amplitudes under single-turnover cond

296 ously aligned duplexes that are extended and unwound within a RecA filament.

297 roximal portion of the leuV promoter that is unwound within the open complex.

298 nded fou2 were found to be lower than in the unwounded WT, but they increased in a similar manner in

299 In unwounded aged skin (versus unwounded younger skin), the level of miR-200c was also

300 meable peroxide by micropipette perfusion to unwounded zebrafish tail fins.