ライフサイエンスコーパス: vigilin

1 hnRNP K homology-domain RNA-binding protein, vigilin.

2 itro-selected RNAs with purified recombinant vigilin.

3 logy to the selected sequences, did not bind vigilin.

4 in Western blot analysis with antibodies to vigilin.

5 ellogenin mRNA-binding protein (VitRNABP) is vigilin.

6 o a family of proteins collectively known as vigilins.

7 ncing revealed that the purified protein was vigilin, a conclusion confirmed in Western blot analysis

8 he function(s) and RNA binding properties of vigilin, a ubiquitous protein with 14 KH domains, remain

9 followed by mass spectrometry and identified vigilin, a ubiquitously expressed multifunctional RNA-bi

10 To probe sites of vigilin action in vertebrate cells, we performed nucleic

11 One KH domain protein of unknown function, vigilin (also known as the high density lipoprotein-bind

12 Vigilin, an evolutionarily conserved KH domain-containin

13 Competition between vigilin and CRD-BP for binding to the CRD may therefore

14 , we highlight the multifaceted functions of vigilin and discuss the findings in the context of gene

15 or binding the 69-nt sequence, through which vigilin and HuR exert opposing effects on c-fms expressi

16 mic-reticulum-localized RNA-binding proteins vigilin and ribosome-binding protein 1 directly bound vi

17 These studies uncover a role for vigilin as a key regulator of hepatic Apob translation a

18 HDLBP, which encodes the RNA-binding protein vigilin, as a candidate tumor suppressor deleted at 2q37

19 edicted and subsequently identified a strong vigilin binding site near the 3' end of human dystrophin

20 The vigilin-binding site in the vitellogenin B1 mRNA 3'-UTR

21 We demonstrated that vigilin binds and negatively regulates MICB expression t

22 Vigilin binds to a region of the vitellogenin mRNA 3'-un

23 Crosslinking studies reveal that vigilin binds to CU-rich regions in the mRNA coding sequ

24 Vigilin binds to the vitellogenin mRNA 3'-UTR site with

25 Vigilin bound to a vitellogenin mRNA 3'-UTR probe with a

26 We report that members of the conserved Vigilin class of proteins have a high affinity for inosi

27 In the presence of RNA, the Vigilin complex recruits the DNA-PKcs enzyme, which appe

28 Molecular complexes including vigilin comprise proteins involved with RNA editing and

29 and genomic approaches, we demonstrate that vigilin controls very-low-density lipoprotein (VLDL) sec

30 that mutation or depletion of the Drosophila Vigilin, DDP1, leads to altered nuclear morphology and d

31 We analyzed binding of RNA targets by vigilin/DDP1/SCP160p and by c-myc coding region instabil

32 Mechanistic studies show that vigilin decreased c-fms mRNA stability.

33 lethal, indicating an essential function for vigilin distinct from its proposed role in chromosome pa

34 Additionally, vigilin downregulation in target cells led to a signific

35 ntial role in chromosome partitioning, human vigilin exhibits a higher affinity for Drosophila dodeca

36 rate the therapeutic potential of inhibiting vigilin for cardiovascular diseases.

37 Whereas recombinant vigilin has no detectable protective effect on PMR-1 cle

38 More recently, we have identified that vigilin in humans plays a critical role in efficient rep

39 s on c-fms expression, suggesting a role for vigilin in suppression of breast cancer progression.

40 These data support a role for vigilin in the hormonal control of mRNA metabolism.

41 function remains unknown, proposed roles for vigilin include chromosome partitioning at mitosis, faci

42 Furthermore, vigilin inhibited c-fms translation.

43 e as a cellular vigilin target through which vigilin inhibits the expression of c-fms mRNA and protei

44 These data show that vigilin is an essential protein in human cells, support

45 Vigilin is essential for cell viability and functions in

46 We have previously reported that vigilin is essential for heterochromatin-mediated gene s

47 Furthermore, nuclear Vigilin is found in complexes containing not only the ed

48 els early in siRNA knockdown indicating that vigilin is not a global regulator of translation.

49 We recently showed that vigilin is the estrogen-inducible protein in polysome ex

50 ogen, providing additional confirmation that vigilin is the estrogen-inducible protein which binds to

51 Direct confirmation that vigilin is the VitRNABP was obtained from RNA gel mobili

52 Here, we show that the RBP vigilin is upregulated in livers of obese mice and in pa

53 le KH-domain proteins, collectively known as vigilins, is indispensable for the heterochromatin-media

54 Consequently, hepatic vigilin knockdown decreases VLDL/low-density lipoprotein

55 tarved, HeLa cells showed that RNAi-mediated vigilin knockdown is rapidly lethal, indicating an essen

56 unaffected by the several fold reduction in vigilin levels early in siRNA knockdown indicating that

57 ailability of purified PMR-1 and recombinant vigilin made it possible to test the hypothesis that RNA

58 Xenopus liver vigilin mRNA and the VitRNABP exhibited similar inductio

59 Altering association of either vigilin or HuR with c-fms mRNA in vivo reciprocally affe

60 trol of translation, and are consistent with vigilin playing a critical role in cytoplasmic mRNA meta

61 Vigilin proteins, the absence of which is known to cause

62 rotein in human cells, support the view that vigilin's most essential functions are neither chromosom

63 Vigilin/Scp160p/DDP1 is a ubiquitous and highly conserve

64 hich demonstrated that antibodies to chicken vigilin supershifted the Xenopus VitRNABP band.

65 Vigilin suppresses while HuR encourages cellular motilit

66 -nt c-fms mRNA 3' UTR sequence as a cellular vigilin target through which vigilin inhibits the expres

67 ences and structures important in binding of vigilin to RNA, before vigilin was purified, we develope

68 mycin and H33342 as inhibitors of binding of vigilin to the vitellogenin mRNA 3'-UTR.

69 portant in binding of vigilin to RNA, before vigilin was purified, we developed a modified in vitro g

70 d structural requirements for interaction of vigilin with RNAs.