ライフサイエンスコーパス: voltage clamp

1 low-voltage inactivation under two-electrode voltage clamp.

2 eriod; and steady-state INa inactivation via voltage clamp.

3 ivalent cations under perfused two-electrode voltage clamp.

4 enopus oocytes, and studied by two-electrode voltage clamp.

5 current-voltage (I-V) relation obtained via voltage clamp.

6 , fura-PE3, to image Ca(2+) under whole-cell voltage clamp.

7 conducting channels determined by whole-cell voltage clamp.

8 ge steps to cells by simultaneous whole-cell voltage clamp.

9 pha(+) cells and activated SK currents under voltage clamp.

10 mbryonic kidney (HEK) cells under whole-cell voltage clamp.

11 evoked currents measured using two electrode voltage clamp.

12 sing human alpha7 nAChR with a two-electrode voltage clamp.

13 effect on net ionic currents measured under voltage clamp.

14 s and ex vivo brain slices, using whole-cell voltage clamp.

15 analogue were studied by using two-electrode voltage-clamp.

16 m cultured bag cell neurons under whole-cell voltage-clamp.

17 proteins, and NCX1 currents using whole-cell voltage clamping.

18 annel recording was performed after 72 hr by voltage-clamping.

19 hDAT (human dopamine transporter) current in voltage-clamped (-60 mV) oocytes consistent with a DAT i

20 on was assayed using dual cell two-electrode voltage clamp after expression in Xenopus oocytes.

21 Whole-cell voltage-clamp analyses in HEK293 cells demonstrated that

22 ey-293 cells, and functions were assessed by voltage clamp analysis.

23 activation was quantified by Ussing chamber voltage clamp analysis.

24 Moreover, presynaptic voltage-clamp analysis revealed increased Ca(2+)-current

25 Voltage-clamp analysis revealed that the NaV1.8/G1662S s

26 Voltage-clamp analysis showed a strong hyperpolarizing (

27 Our voltage-clamp analysis showed that R1279P depolarizes ch

28 ng of their functional properties comes from voltage-clamp analysis, the predominant approach for inv

29 ted the kinetic properties of the mutants by voltage clamp and (32)P uptake.

30 In this study, using whole cell voltage clamp and mechanical measurement techniques, we

31 n Xenopus laevis oocytes using two-electrode voltage clamp and on Cx43 and Cx46 expressed in HeLa cel

32 is oocyte expression system by two-electrode voltage clamp and optical osmotic swelling assays.

33 responses were recorded by using 2-electrode voltage clamp and single-channel electrophysiology, wher

34 Experiments in voltage clamped and field stimulated ventricular myocyte

35 (LSO) from postnatal day 7 (P7) to P96 using voltage-clamp and auditory brainstem responses.

36 Using voltage-clamp and cAMP-protein kinase A (PKA) FRET senso

37 Using acutely isolated neurons, mixed voltage-clamp and current-clamp experiments were done at

38 Using whole-cell voltage-clamp and current-clamp recordings in acute hipp

39 in adult skeletal muscle fibers subjected to voltage-clamp and on RyR1 channel activity after incorpo

40 Each methylnicotine was investigated using voltage-clamp and radioligand binding techniques.

41 e isolated from CeA neurons under whole-cell voltage clamp, and their response to selective BK channe

42 urrents were recorded using 2-microelectrode voltage clamping, and surface expression was analyzed by

43 Combining multielectrode array, voltage-clamp, and current-clamp recordings, we assessed

44 T channels expressed in Xenopus oocytes were voltage-clamped, and distinct LRET signals were obtained

45 adients in the LUVs in the same manner as in voltage-clamped animal cells.

46 ively measured beyond the limitations of the voltage-clamp approach using fast Ca(2+) imaging with lo

47 of directional inhibition, as required for a voltage-clamp artifact.

48 Here, we test this voltage-clamp-artifact hypothesis in recordings from 62

49 us laevis oocyte-based automated 2-electrode voltage clamp assay.

50 We used a voltage-clamp assay on Xenopus oocytes injected with the

51 per CSF sample was performed in a whole-cell voltage-clamp assay.

52 sured in Xenopus oocytes using two-electrode voltage clamp assays.

53 an isophtalate (SBFI) AM from unperturbed or voltage-clamped astrocytes and respective glutamate tran

54 in Xenopus oocytes by using a two-electrode voltage clamp at -100 mV.

55 We probed Ca(2+) activation without voltage clamp by applying Na(+)-free (0 Na(+)) solution

56 fect on the degree of Ca(2+) alternans in AP voltage-clamped cells, confirming that suppression of Ca

57 rge and small alternans CaTs were applied to voltage-clamped cells.

58 Na(+) fluxes measured in vitro under voltage clamp conditions in controls demonstrate that bo

59 Under voltage clamp conditions, ATP activated large outward cu

60 Responses recorded under voltage-clamp conditions had identical short latencies a

61 Under voltage-clamp conditions in outside-out patches, this cr

62 bed by a continuum electrostatic model under voltage-clamp conditions, the control of ion flow by the

63 lifetime of ASAP1, CAESR, and ArcLight under voltage-clamp conditions.

64 richia coli and in single HEK293 cells under voltage-clamp conditions.

65 RyR1 into planar phospholipid bilayers under voltage-clamp conditions.

66 mbrane potential in complex cells, since the voltage-clamp configuration constrains the membrane pote

67 rrent measurements using the Cut-open Oocyte Voltage Clamp (COVC) technique.

68 nd release assays in rat brain synaptosomes, voltage-clamp current measurements in cells expressing t

69 neuron, we compared simulation results under voltage-clamp, current-clamp and high [K(+)] membrane de

70 Here, using structural modeling, voltage-clamp, current-clamp, and multielectrode array r

71 Together, these voltage-clamp data suggest that a number of release proc

72 nopus oocytes and tested using two-electrode voltage clamp, demonstrated an increase in ethanol sensi

73 Well-coupled CTL myocytes are effectively voltage-clamped during Ca waves, protecting the heart fr

74 In whole-cell voltage clamp, Dyn-A opened G-protein-coupled inwardly r

75 We performed voltage clamp electrophysiological experiments to study

76 Using two-electrode voltage clamp electrophysiology in Xenopus laevis oocyte

77 Xenopus oocyte expression and two-electrode voltage clamp electrophysiology, we found that co-expres

78 m function were measured using two-electrode voltage clamp electrophysiology.

79 the Cys mutants was confirmed by whole-cell voltage clamp electrophysiology.

80 y and in Xenopus oocytes using two-electrode voltage clamp electrophysiology.

81 Xenopus oocytes and assayed by two-electrode voltage clamp electrophysiology.

82 rologous expression systems using whole-cell voltage-clamp electrophysiology and immunohistochemistry

83 ed cytisine derivatives, using two-electrode voltage-clamp electrophysiology and noncanonical amino a

84 Using two-electrode voltage-clamp electrophysiology in Xenopus laevis oocyte

85 Using two-electrode voltage-clamp electrophysiology in Xenopus oocytes, oxyt

86 Using voltage-clamp electrophysiology of heterologously expres

87 Two-electrode voltage-clamp electrophysiology revealed that, when coex

88 Voltage-clamp electrophysiology studies of alpha1beta2M2

89 oiodinated alpha-bungarotoxin, two-electrode voltage-clamp electrophysiology, and calcium imaging.

90 in Xenopus laevis oocytes and two-electrode voltage-clamp electrophysiology, and radiolabeled substr

91 Using two-electrode voltage-clamp electrophysiology, fast-application patch-

92 To overcome these voltage-clamp errors, we developed an approach to provid

93 Voltage clamp experiments showed activation of inward cu

94 In oocytes, two-electrode voltage clamp experiments showed that CYBDOM-mediated cu

95 neurons, 100 nm GxTX-1E broadened spikes and voltage clamp experiments using action potential wavefor

96 This finding is directly validated in voltage clamp experiments with Ca waves using isolated r

97 ecular dynamics, cysteine cross-linking, and voltage clamp experiments, we propose a dynamics-driven

98 n Xenopus laevis oocytes using two-electrode voltage clamp experiments.

99 GABAAR isoforms consistent with results from voltage-clamp experiments (EC50 values for alpha4beta3de

100 Computational analyses and voltage-clamp experiments measuring L-type Ca(2+) curren

101 Dual voltage-clamp experiments substantiate low resistance el

102 ed gene variant was functionally analyzed by voltage-clamp experiments using various heterologous cel

103 In voltage-clamp experiments, 2-AG reduced A-type potassium

104 activity using interleaved current-clamp and voltage-clamp experiments.

105 e in I(KS) channel function was confirmed by voltage-clamp experiments.

106 transmitter release, we combined presynaptic voltage clamp, fluorescence imaging, electron microscopy

107 annel recordings, cysteine accessibility and voltage clamp fluorimetry to probe the relationships bet

108 Using voltage-clamp fluorimetry and gating current analysis, w

109 Voltage-clamp-fluorimetry studies also indicated that in

110 Voltage clamp fluorometry (VCF) allows simultaneous meas

111 We used voltage clamp fluorometry (VCF) and molecular dynamics (

112 Voltage clamp fluorometry measurements combining electro

113 Voltage clamp fluorometry revealed that the voltage-sens

114 In this study, we use the voltage clamp fluorometry technique to identify the mole

115 We here used voltage clamp fluorometry to define how the homologous P

116 Here, we use voltage clamp fluorometry to determine how KCNE1 and KCN

117 Here, we use voltage clamp fluorometry to determine how KCNE3 affects

118 Using voltage clamp fluorometry, we find that the acidic pocke

119 In the present study, we used voltage-clamp fluorometry (VCF) to measure conformationa

120 Patch-clamp and voltage-clamp fluorometry combine spectroscopic and elec

121 Voltage-clamp fluorometry experiments and kinetic modeli

122 Voltage-clamp fluorometry experiments indicate that both

123 Although voltage-clamp fluorometry fills this gap, it is limited

124 using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements were performed wi

125 Voltage-clamp fluorometry revealed that the E176K substi

126 Voltage-clamp fluorometry showed a loss of a fast compon

127 We used voltage-clamp fluorometry to study conformational change

128 upled to changes in voltage sensing, we used voltage-clamp fluorometry to track conformational change

129 Voltage-clamp fluorometry was used to record ion channel

130 Here, we solved this problem by performing voltage-clamp fluorometry with a fluorescent unnatural a

131 Using an optical approach (voltage-clamp fluorometry) to track the movement of the

132 e employ a combination of electrophysiology, voltage-clamp fluorometry, synthetic BigDyn analogs, and

133 Using voltage-clamp fluorometry, we here detect two conformati

134 Using voltage-clamp fluorometry, we show that AHA incorporatio

135 tate KCNQ1 conductance is demonstrated using voltage-clamp fluorometry.

136 onformational changes within each VSD, using voltage-clamp fluorometry.

137 rophysiology, site-directed mutagenesis, and voltage-clamp fluorometry.

138 cell anion channel activity, recorded under voltage clamp, follows KfSLAC1 and KfALMT12 transcript a

139 responses were recorded in current clamp and voltage clamp from olig2(+) neurons in immobilized larva

140 Conductance through single, voltage-clamped fusion pores directly reported sub-milli

141 ed transmitter release from MOC terminals in voltage-clamped IHCs in the whole-cell configuration.

142 is of the capacitive currents obtained under voltage clamp in molecular layer interneurons of juvenil

143 In voltage-clamped, intact Casq2-/- cardiomyocytes pretreat

144 Here, we reveal that voltage clamp is completely ineffective for most excitat

145 o the membrane (confocal imaging, whole-cell voltage-clamp, K5fluo-4 as Ca(2+) indicator).

146 w (tau approximately 350 ms) tail current in voltage-clamped light responses and show that it is medi

147 light pulse duration, the typically reported voltage-clamp-measured ChR2 current traces are often not

148 Here we used two-electrode voltage clamp measurements in Xenopus oocytes together w

149 cement of delayed rectifier K(+) currents in voltage clamp measurements observed at least 3 h followi

150 in combination with confocal microscopy, and voltage-clamp measurements of hyperpolarizing currents,

151 sed by Ca(2+) imaging, (86)Rb(+) efflux, and voltage-clamp measurements.

152 We used the 2-electrode voltage-clamp method to quantify responses to acetylchol

153 293 cells were recorded using the whole-cell voltage-clamp method.

154 In this study, using whole cell voltage clamp methodology, the actions of dexmedetomidin

155 high levels in plasma membrane and applying voltage clamp methods, Eriksen et al. (2016) have identi

156 w-pass filtering, whereas light responses in voltage-clamp mode produced bandpass filtering in all ON

157 (+)-K(+) pump current, I(p), was measured in voltage-clamped myocytes from noninfarct myocardium.

158 quivalent to those expected to be imposed on voltage-clamped myocytes supported this hypothesis.

159 ld in LPP field potential studies but not in voltage clamped neurons; coupled with input/output relat

160 or, evoking no changes in holding current in voltage-clamped neurons and showing an IC50 of at least

161 EtOH increased the holding current of voltage-clamped neurons and this action was blocked by p

162 Using whole-cell voltage clamp of Nav1.6, we show that CaMKII inhibition

163 each of the mutant channels was assessed by voltage clamp of oocytes using micropipettes filled with

164 Voltage clamp of outside-out patches from L2/3 neurons r

165 Both computer simulation and in vivo dynamic voltage clamp of spinal motor neurons in septic rats wer

166 ) channel conductance measured by whole-cell voltage clamp of the same cell.

167 Using studies in both sperm and voltage clamp of Xenopus oocytes, we define a molecular

168 dvantages for biophysical studies, including voltage-clamp of both pre- and postsynaptic compartments

169 ynaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the

170 In voltage-clamp oocyte studies using the ubiquitous amanta

171 solution imaging to track single vesicles at voltage-clamped presynaptic terminals of retinal bipolar

172 kept this neuron silent or used a long-term voltage clamp protocol to artificially maintain activity

173 We measured these currents using a voltage-clamp protocol and then estimated the energetic

174 t trace from a short and rapidly fluctuating voltage-clamp protocol.

175 Application of voltage clamp protocols in the form of pre-recorded APs

176 These machines can execute traditional voltage-clamp protocols aimed at specific gating process

177 ical electrophysiology, we recapitulate many voltage-clamp protocols and apply to Nav1.7, a channel i

178 ion channels typically involve sophisticated voltage-clamp protocols applied through manual or automa

179 ta recorded using standard electrophysiology voltage-clamp protocols that have not been developed wit

180 annotated metadata and responses to a set of voltage-clamp protocols, we assigned 2378 models of volt

181 g hyperpolarization preceding a depolarizing voltage-clamp pulse delayed the rise of the potassium co

182 Experiments were performed using Fluo-3 in voltage clamped rat ventricular myocytes.

183 We measured [Ca(2+)]i with fluo-3 in voltage-clamped rat ventricular myocytes.

184 2)2-nAChRs was confirmed using two-electrode voltage clamp recording of responses to nicotinic ligand

185 channel contribution to outward currents in voltage clamp recordings as determined by pharmacologica

186 Voltage clamp recordings from mEC stellate cells in rat

187 transmission was investigated in whole-cell voltage clamp recordings from medium spiny neurons of th

188 Voltage clamp recordings from SCs nucleated patches show

189 unds were evaluated using both two-electrode voltage clamp recordings from Xenopus laevis oocytes and

190 We made two-electrode voltage clamp recordings from Xenopus laevis oocytes exp

191 Using whole-cell voltage clamp recordings in brain slice preparations, th

192 In whole cell voltage clamp recordings of HEK293 cells, wild-type but

193 Synaptic measurements in whole cell voltage clamp recordings of inspiratory neurons revealed

194 pressed in Xenopus oocytes and two-electrode voltage clamp recordings used to investigate the effects

195 In vitro intracellular current clamp and voltage clamp recordings were performed in muscle from a

196 Whole-cell voltage clamp recordings were then made from CA1 pyramid

197 t through them was measured using whole cell voltage clamp recordings.

198 Voltage-clamp recordings demonstrate a hyperpolarising s

199 Here, we perform voltage-clamp recordings from axial motoneurons in larva

200 Nonetheless, whole-cell voltage-clamp recordings from cdko rods revealed a profo

201 Whole-cell voltage-clamp recordings from cGOF or icGOF ventricular

202 Here we present voltage-clamp recordings from inner hair cells of the C5

203 assium current was measured using whole-cell voltage-clamp recordings from KF and locus coeruleus (LC

204 Using voltage-clamp recordings from nearby pyramidal cells, we

205 mission, we investigated Cplx function using voltage-clamp recordings from postsynaptic horizontal ce

206 4 function in photoreceptor ribbon synapses, voltage-clamp recordings from postsynaptic horizontal ce

207 Using whole-cell voltage-clamp recordings from rat calyx of Held presynap

208 Combined current- and voltage-clamp recordings from the same cell showed the s

209 Here, whole-cell voltage-clamp recordings from VB neurones of mouse thala

210 Here, whole-cell voltage-clamp recordings from VB neurones of mouse thala

211 Voltage-clamp recordings further revealed that although

212 In voltage-clamp recordings in brain slices from adult mice

213 of Held, normally show strong depression in voltage-clamp recordings in brain slices.

214 Whole-cell voltage-clamp recordings in human embryonic kidney (HEK)

215 Whole-cell voltage-clamp recordings in small DRG neurons demonstrat

216 Whole-cell voltage-clamp recordings obtained after 3-7 days in cult

217 ences between rod flash responses and recent voltage-clamp recordings of cone flash responses, using

218 We show in cut-open oocyte voltage-clamp recordings of gating and ionic currents of

219 Whole-cell voltage-clamp recordings of mEPSCs in CA1 pyramidal neur

220 Voltage-clamp recordings of miniature EPSCs (mEPSCs) fro

221 ying the behavioural hyperalgesia phenotype, voltage-clamp recordings of small and medium dorsal root

222 Voltage-clamp recordings of the mutant KCNQ3 channels re

223 Two-electrode voltage-clamp recordings of Xenopus laevis oocytes expre

224 el suggesting that apparent DS excitation in voltage-clamp recordings results from inadequate voltage

225 Voltage-clamp recordings reveal that effective feedforwa

226 Whole-cell voltage-clamp recordings revealed a concomitant nonquant

227 Whole-cell voltage-clamp recordings revealed that blocking the ON p

228 Voltage-clamp recordings revealed that the Nav1.7-A1632G

229 Whole-cell voltage-clamp recordings revealed that tone-evoked synap

230 Finally, voltage-clamp recordings showed that FMRP modulated I(h)

231 unds by means of Hille-Campbell Vaseline gap voltage-clamp recordings showed that the elongation of t

232 Voltage-clamp recordings showed that TNFalpha had no eff

233 In voltage-clamp recordings the outward current produced by

234 Here, we used voltage-clamp recordings to evaluate the effects of laco

235 Whole-cell current- and voltage-clamp recordings were made from isolated guinea

236 Whole-cell voltage-clamp recordings were obtained from layer V pyra

237 ation condition and using whole-cell somatic voltage-clamp recordings, the amplitudes and kinetics of

238 In this study, using in vivo whole-cell voltage-clamp recordings, we revealed eye-specific excit

239 rents is routinely achieved using whole-cell voltage-clamp recordings.

240 es derived from synaptic currents in in vivo voltage-clamp recordings.

241 nd abnormally large R currents in whole-cell voltage-clamp recordings.

242 Whole-cell voltage clamping revealed that expression of CNNM3 influ

243 Two-electrode voltage clamp showed dose-dependent block of the AQP1 io

244 pus laevis oocytes followed by two-electrode voltage clamp showed that TcPho91 is a low-affinity tran

245 tions that determine the thermal motion of a voltage-clamped single-stranded DNA-NeutrAvidin complex

246 Under local dendritic voltage clamp, single-spine activation produced large sp

247 in nonphysiological conditions (square-pulse voltage clamp, slow pacing rates, exogenous Ca(2+) buffe

248 inetics was limited by three factors: (1) HC voltage-clamp speed, (2) cone voltage-clamp speed, and (

249 actors: (1) HC voltage-clamp speed, (2) cone voltage-clamp speed, and (3) kinetics of Ca(2+) channel

250 Under voltage clamp, step depolarizations evoke transient Na(+

251 Furthermore, in two-electrode voltage clamp studies in Xenopus oocytes, both Ca(2+) an

252 Fluorescence assays and voltage clamp studies reveal that the self-assemblies in

253 Results of voltage-clamp studies revealed presynaptic defects chara

254 es using a Xenopus oocyte two-microelectrode voltage clamp system revealed mutations with only loss-o

255 Using the two-electrode voltage clamp technique and alpha4beta2 nAChRs in the Xe

256 the range of 1-780 nm) by the two-electrode voltage clamp technique using a standard Xenopus oocyte

257 Using the cut-open voltage clamp technique, we have simultaneously recorded

258 opus laevis oocytes using two-microelectrode voltage clamp technique.

259 1) density was measured using the whole-cell voltage clamp technique.

260 sing immunoblot analysis and a two-electrode voltage clamp technique.

261 e characterized using the two-microelectrode voltage clamp technique.

262 the current study, we used the two-electrode voltage-clamp technique alone or in combination with pH/

263 Ce1-mediated currents with the two-electrode voltage-clamp technique or pHi changes using Vm/pH-sensi

264 this study we have used the cut-open oocyte voltage-clamp technique to investigate the relationship

265 nels expressed in Xenopus oocytes, using the voltage-clamp technique.

266 s were measured using the two-microelectrode voltage-clamp technique.

267 s on GABAAR by means of a two-microelectrode voltage-clamp technique.

268 urements were combined with action potential voltage clamp techniques in a physiologically relevant h

269 3)(beta(2))(2) receptors using two-electrode voltage clamp techniques in Xenopus laevis oocytes indic

270 e injected Xenopus oocyte with two-electrode voltage clamp techniques to characterize the action of A

271 llular compartments, and (b) patch clamp and voltage clamp techniques, which investigate transporters

272 sing quantitative swelling and two-electrode voltage clamp techniques.

273 tory currents were recorded using whole cell voltage clamp techniques.

274 Voltage-clamp techniques have revolutionized clinical el

275 In this study, we use whole-cell voltage-clamp techniques to analyze light responses of i

276 To elucidate the mechanisms of IH, we used voltage-clamp techniques to investigate the [H]o, [Na]o,

277 Whole-cell voltage-clamp techniques were used to study the alterati

278 e therefore recorded lidocaine inhibition of voltage-clamped, tetrodotoxin-sensitive Na currents in m

279 sured in Xenopus oocytes using two-electrode voltage clamp (TEV) assays.

280 Two-electrode voltage clamp (TEVC) of transfected Xenopus oocytes reve

281 ed using cell-based assays and two-electrode voltage clamp (TEVC) technique on M2 channels, respectiv

282 identify the channel, we use whole-mitoplast voltage-clamping, the technique that originally establis

283 We used voltage clamp to characterize synaptic variability.

284 ogether, using Archaerhodopsin as an optical voltage clamp to provide the driving force for chloride

285 We used whole-cell voltage clamping to compare the biophysical parameters o

286 ile of the biological PD neuron, measured in voltage clamp, to constrain parameter values of a conduc

287 Voltage-clamp using ramp or repetitive action potentials

288 d a transient current beyond steady state in voltage-clamped ventricular myocytes as reported by othe

289 n Xenopus oocytes and the two-microelectrode voltage clamp was used to measure the kinetics and stead

290 Using ultrafast perfusion with voltage clamp, we applied glutamate to outside-out patch

291 Using whole-cell voltage clamp, we discovered that enhancing S-palmitoyla

292 Using two-electrode voltage clamp, we recorded from oocytes that were inject

293 Using single-electrode voltage clamp, we show that brief changes of ComInt 1's

294 Using whole-cell voltage-clamp, we have identified a typical M-current wi

295 tes expression system and two microelectrode voltage-clamp, we report the functional expression and t

296 text] binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyR

297 opus oocytes, were measured by two-electrode voltage clamp, whereas the native background K(+) conduc

298 (mEPCs) and nerve-evoked EPCs (eEPCs) under voltage-clamp, which, unlike current-clamp records, were

299 currents in Xenopus oocytes using a cut-open voltage-clamp with extracellular solution titrated to ei

300 opening voltage-gated calcium channels under voltage clamp, without affecting the number of synaptic