ライフサイエンスコーパス: wild-type gene

1 lative to the same strain that expressed the wild type gene.

2 ognosis as compared to patients carrying the wild type gene.

3 600E) in the presence of 20-fold more of the wild-type gene.

4 omplemented by a plasmid-encoded copy of the wild-type gene.

5 ant dgt gene on the chromosome replacing the wild-type gene.

6 mbination into the chromosome, replacing the wild-type gene.

7 oss of function as compared with that of the wild-type gene.

8 ained as plasmids unless complemented by the wild-type gene.

9 ed this deficiency in transfectants with the wild-type gene.

10 m mutation but from elevated expression of a wild-type gene.

11 s of the gene can reconstruct a functioning, wild-type gene.

12 y of the mutant gene was used to replace the wild-type gene.

13 or physiological role to be assigned to the wild-type gene.

14 nce by complementation with their respective wild-type gene.

15 zygotic for this change or those who had the wild-type gene.

16 restored by genetic complementation with the wild-type gene.

17 mutation was complemented in trans with the wild-type gene.

18 ns showed the same outcome as those with the wild-type gene.

19 orm cannot accomplish many activities of the wild-type gene.

20 tably transfected derivatives expressing the wild-type gene.

21 , we isolated genomic and cDNA clones of the wild-type gene.

22 tandem repeat array inserted upstream of the wild-type gene.

23 P that alters the stop codon of an otherwise wild-type gene.

24 and complementation of this mutant with the wild-type gene.

25 ued by replacement of the cDNA copy with the wild-type gene.

26 restored upon complementation with the embC wild-type gene.

27 nsulin, respectively, than mice carrying the wild-type gene.

28 nal regulation is a critical function of the wild-type gene.

29 itivity was restored by complementation with wild-type genes.

30 defects by plasmid clones of the respective wild-type genes.

31 lly expressing the corresponding Arabidopsis wild-type genes.

32 es, including all combinations of mutant and wild-type genes.

33 ith loss of the CH-42 transgene and adjacent wild-type genes.

34 tivity to the same extent as the 196-residue wild-type gene 1.7 protein.

35 form dimers at salt concentrations where the wild-type gene 2.5 protein exists as a dimer.

36 itive mutant revealed a 21-bp duplication of wild-type gene 37 inserted into its C-terminal portion.

37 dual deletion strains with the corresponding wild-type gene abolished cisplatin resistance, confirmin

38 an adenoviral construct encoding the IKKbeta wild-type gene (Ad.IKKbeta-wt); controls received an ade

39 develop in people bearing one mutant and one wild-type gene allele.

40 ly mosaic worms, in which some cells carry a wild-type gene and others are homozygous mutant, can rev

41 species, Nicotiana clevelandii, retains the wild-type gene and produces nanamins.

42 ein was the same size as that encoded by the wild-type gene and that both the wild-type and mutated p

43 s caused by recombination events between the wild-type gene and the pseudogene(s).

44 ntervals: patients showing the best outcome (wild-type gene and unaltered protein), an intermediate o

45 nown cancer-driving pathways, we analyzed 86 wild-type genes and 94 mutated variants for their effect

46 ly acting exogenous genes, overexpression of wild-type genes, and Cre recombinase-mediated gene ablat

47 Whereas both mutant and wild-type genes appeared to be transcribed and spliced e

48 ed)1 mutants indicate that the corresponding wild-type genes are required to repress root phenylpropa

49 specific genetic interactions using a cloned wild-type gene as a starting point.

50 ing loss of function, many others maintain a wild-type gene but exhibit reduced p53 tumor suppressor

51 n was complemented by the plasmid-borne yDHS wild-type gene, but not by mutated genes encoding inacti

52 ve-site mutations, and overexpression of the wild-type gene, but not the catalytic-site mutant, parti

53 uch weaker, basal level transcription of the wild type gene by RNA polymerase II under non-heat shock

54 We cloned the wild-type gene by complementation of the temperature-sen

55 endonuclease gene; (3) back-crosses with the wild-type gene by ligation to the wild-type N-terminal o

56 Expression of the wild-type gene (CAMV35S::ECA1) reversed these conditiona

57 lectivity - one mutant in 1000 copies of the wild-type gene can be detected.

58 rthenogenesis, which indicates that a single wild-type gene can bypass the fertilization checkpoint i

59 within BLM can occur to form a functionally wild-type gene capable of correcting the mutant phenotyp

60 Overexpression of the wild-type gene causes cell death and disrupts the normal

61 cterized HCMV strain containing the complete wild-type gene complement and promises to enhance the cl

62 Thus, HCMV containing a wild-type gene complement can be generatedin vitroby der

63 pase-resistant GATA-1 mutant, but not of the wild-type gene, completely restored erythroid expansion

64 e can be rescued by complementation with the wild-type gene, consistent with a function for AtNBS1 in

65 simplification allows one to deduce how the wild-type gene contributes to patterning the normal, mor

66 since they all contained both disrupted and wild-type gene copies, and expression of functional GbpB

67 DNA walks for wild-type genes demonstrate varying levels of chaos, wit

68 urrent than coexpressing the mutant with the wild-type gene, demonstrating that the polymorphism resc

69 4.0-5.4 months), patients carrying the TLR4 wild type gene displayed cardiac recovery under intense

70 f active site mutants of DsbA instead of the wild-type gene do not produce this increase in yield.

71 plication of 270 kb that includes the entire wild-type gene encoding the tight junction protein TJP2

72 coenzyme A pathway intermediates or with the wild-type gene encP restored the formation of the benzoa

73 A2 strain G gene (one stop codon preceding a wild-type gene end signal), an A4G gene end signal prece

74 E RESPONSE1 and requires the function of the wild-type genes ETHYLENE INSENSITIVE2 (EIN2), EIN4, AUXI

75 Mutant strains, transformed with a copy of a wild-type gene, exhibited a macrophage survival level si

76 We found 17 modes of wild-type gene expression and refined them into 57 submo

77 ssion of the mutant allele without affecting wild-type gene expression could be a powerful new treatm

78 n anatomical structure; direct access to the wild-type gene expression data for the tissues affected

79 on of Mist1 in adult Mist1(KO) mice restored wild-type gene expression patterns in acinar cells.

80 chd mRNA into the early embryo, we restored wild-type gene expression patterns, and the resultant fi

81 The model is able to reproduce the wild-type gene expression patterns, as well as the ectop

82 The wild-type gene for alanine dehydrogenase, incorporated i

83 Replacement of five codons in the wild-type gene for antigen 85B increased recombinant pro

84 omes are prepared from s strain carrying the wild-type gene for PAP I (pcnB+).

85 Complementation of the cysA mutant with the wild-type gene from M. tuberculosis restored prototrophy

86 A cloned wild-type gene from this region, abgT (formerly ydaH) co

87 ene for damage avoidance, i.e., to produce a wild-type gene from two nonfunctional allelic copies of

88 ngth could be complemented by expressing the wild-type genes from a plasmid.

89 e assay, based on a visible test for loss of wild-type gene function at a single locus, golden, is re

90 d efficient method for obtaining clues about wild-type gene function.

91 ss morphological phenotypes, not because the wild-type gene functions pleiotropically in several sign

92 about 20-fold brighter fluorescence than the wild-type gene (gfp).

93 x Normal, suggesting that both copies of the wild-type gene had undergone RIP.

94 The complementing wild-type gene has been cloned and and shown to encode a

95 roteins but is a mix of proteins codified by wild-type genes; (iii) the form is biochemically classif

96 vity of a cdc14-1(ts) mutant nor replace the wild type gene in vivo, demonstrating that phosphatase a

97 ype that can be rescued by expression of the wild-type gene in clock neurons, indicating a role for S

98 s found the coding region unchanged from the wild-type gene in each case, but variation was observed

99 verified by complementation with the cloned wild-type gene in in vitro and in vivo assays.

100 the rad53 mutant, suggesting a role for the wild-type gene in maintaining chromosome integrity in th

101 , and phenotypic rescue by expression of the wild-type gene in rdn1 mutants.

102 The mutant strain was complemented with the wild-type gene in single copy.

103 Single mutants were complemented with the wild-type gene in trans, showing restoration of the wild

104 uld be complemented by the expression of the wild-type gene in trans.

105 eover, expressing GRK4gammaA142V but not the wild-type gene in transgenic mice produces hypertension

106 Expression of the wild-type gene in TSC2 mutant tumor cells inhibits proli

107 d by UUG was also able to substitute for the wild-type gene in vivo in yeast.

108 ied that it encodes C3H by expression of the wild-type gene in yeast.

109 this clone were made and used to replace the wild-type genes in the bacterial chromosome by marker ex

110 ene knocked out in the micronucleus but only wild-type genes in the polycopy somatic macronucleus.

111 ant was complemented with the representative wild-type genes in trans to restore expression of parent

112 eculiarity of TCRbeta, as we identified many wild-type genes, including one essential for NMD, that t

113 heme-dependent manner similar to that of the wild-type gene, indicating that control by those effecto

114 these regulatory sequences are removed, the wild-type gene is expressed at high levels.

115 detect 1 codon 61 mutant allele among 10,000 wild-type genes, is described.

116 This finding indicated that the wild-type gene junction has evolved to achieve the maxim

117 reby heterozygous coexpression of mutant and wild-type genes leads to more serious pathology than is

118 to understanding the mechanisms by which the wild-type genes lose activity.

119 ved cells transduced with virus carrying the wild-type gene maintained normal blood counts following

120 Experiments demonstrate that the wild-type gene Mop1 is required for establishment and ma

121 The wild-type gene, named mucK, was cloned into pUC18, and i

122 reports on the essentiality of both the yrdC wild-type gene of Escherichia coli and the SUA5 wild-typ

123 d-type gene of Escherichia coli and the SUA5 wild-type gene of Saccharomyces cerevisiae led us to rec

124 The present data show that the wild-type genes of two members of the Halloween family o

125 ype was rescued by the reintroduction of the wild-type gene on a plasmid, proving that the hyphal gro

126 the gene of interest and used to replace the wild-type gene on the chromosome by allelic exchange.

127 hese mutant alleles were used to replace the wild-type gene on the streptococcal chromosome, analysis

128 ectly test their function, we introduced the wild-type genes on high-copy plasmids into Escherichia c

129 osits are derived from precursors encoded by wild-type genes (perhaps influenced by alleles that are

130 allows AVP secretion, albeit less than with wild-type gene precursor.

131 The nature of the wild-type gene product at the mouse ichthyosis (ic) locu

132 ponding mutant, agp30, has revealed that the wild-type gene product is required in vitro for root reg

133 enotype of the digenic rga/gal-3 mutant, the wild-type gene product of RGA is probably a negative reg

134 Overexpression or misexpression of a wild-type gene product, however, can also cause mutant p

135 E. coli strains that allow depletion of the wild-type gene product.

136 with the function of the wild-type mtDNA or wild-type gene products.

137 rms are used to describe three attributes of wild-type gene products: their molecular function, the b

138 e phenotype of the deletion strains with the wild-type genes proves that the sensitivity of the strai

139 Complementation of an hp310 strain with the wild type gene restored lysozyme resistance.

140 n of the F1 DeltamcpC mutant XLF004 with the wild-type gene restored chemotaxis to cytosine.

141 t strain by chromosomal reintegration of the wild-type gene restored expression of this murein hydrol

142 Complementation of the deletion with the wild-type gene restored full virulence.

143 lementation of P. gingivalis FLL455 with the wild-type gene restored the level of NO sensitivity to a

144 ion of mutant lines with their corresponding wild-type genes restored picolinate auxin sensitivity.

145 Plasmids carrying the complementary wild-type genes restored the ability of mutant strains t

146 of the 1818(PE_PGRS)::Tn5367 mutant with the wild-type gene restores both aggregative growth (clumpin

147 any inconsistency between the entry and the wild type gene sequence.

148 Comparison of mutant and wild-type gene sequences in this region revealed a G-to-

149 e silencing of transcription from methylated wild-type gene sequences was demonstrated.

150 growth and a distinct color, but maintains a wild-type gene set and the capacity for photosynthesis.

151 Molecular analysis of the wild-type gene showed that this process involves gene co

152 2 gene revealed a pattern different from the wild-type gene, suggesting a mutation in the sod2 gene.

153 The respective wild-type genes suppress the peroxide sensitivities of s

154 We replaced the wild-type gene that encodes the NADP-dependent IDH of Es

155 revealed a G to A transition relative to the wild-type gene that would result in the substitution of

156 transferred to the chromosome, replacing the wild-type gene, the cells became inviable.

157 s the correction of a mutated allele back to wild-type ("gene therapy").

158 In contrast to the wild-type gene, this mutant has no effect on cell clonog

159 nabled us to measure the rate of loss of the wild-type gene through deletion, recombination, or gene

160 nuclease) cleaved the imperfectly hybridized wild-type gene together with all other mismatched sequen

161 valuation when compared with carriers of the wild type gene under the same treatment conditions.

162 les revealed that the ATG start codon of the wild-type gene was converted to the rare GTG start codon

163 Second, a wild-type gene was disrupted by the insertion of a marke

164 nd deficiency mapping, and the corresponding wild-type gene was isolated in a molecular walk.

165 The wild-type gene was restored in the mutant by homologous

166 However, in the wild type gene we have found that CPDs are efficiently r

167 ect that could be complemented by the cloned wild-type gene, we have designated EF1809 ebrA (enteroco

168 Mutant and wild-type genes were cloned into a Leishmania-specific v

169 use model of this condition by replacing the wild type gene with one encoding Kcnt1(R455H), a cytopla

170 sulted in the replacement of the rickettsial wild-type gene with a partially deleted pld gene.

171 R) in embryonic stem (ES) cells to replace a wild-type gene with a slightly modified one.

172 a complementing chromosomal insertion of the wild-type gene with its indigenous promoter at a second