ライフサイエンスコーパス: zein

1 2.00474 found in model system prepared with zein.

2 ritionally limited storage proteins known as zein.

3 at FL1 DUF593 interacts with the 22-kD alpha-zein.

4 e not observed in multiprotein networks with zein.

5 significantly exacerbated by ATIs and not by zein.

6 hem interacted strongly with the 10-kD delta-zein.

7 interaction of resveratrol with gliadin and zein.

8 her temperatures, which was not observed for zein.

9 lloidal particles prepared from food protein-zein.

10 y, and a soft, floury endosperm deficient in zeins.

11 indicating their hypostatic action to gamma-zeins.

12 ptides, the alpha-, beta-, gamma-, and delta-zeins.

13 pha- and delta-zeins and the beta- and gamma-zeins.

14 ughout the endosperm before alpha- and delta-zeins.

15 ining protein bodies, similar to other gamma-zeins.

16 Zein, a plant protein obtained from corn, is a useful bi

17 biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with gly

18 ovide data on ileal digestibility of WPI and zein AAs in healthy humans and, in contrast to WPI, zein

19 whole endosperm, thus indicating that delta-zein adheres to granule surfaces after disruption of the

20 We found that genes encoding zeins, alpha-globulin, and legumin-1 are transcribed not

21 trait locus and may suggest the 50-kD gamma-zein also contributes to this quantitative trait locus.

22 identified proteins, including a 50-kD gamma-zein, an 18-kD alpha-globulin, and a legumin-related pro

23 al mutant, opaque 2 (o2) causes reduction of zeins, an increase of nonzein proteins, and as a consequ

24 h this, all ESTs derived from several genes (zein and adh1) that are known to be exclusively expresse

25 The possible interactions between alpha-zein and Ca(2+) in nixtamalization process were analyzed

26 proteases) and a few storage genes (an alpha-zein and caleosin), which are good candidates for develo

27 s was also explored with networks containing zein and either soy or pea protein isolates as supplemen

28 The content of alpha-zein and gamma-zein was measured in pools of high- and l

29 sco-elastic masses could be formed from both zein and kafirin preparations by coacervation from glaci

30 Stress-relaxation analysis of coacervated zein and kafirin visco-elastic masses showed they were i

31 The content of alpha-zein and several cytoskeletal proteins was measured in h

32 facilitating the localization of 22-kD alpha-zein and that this is essential for the formation of vit

33 esting an important role for the 16-kD gamma-zein and the 15-kD beta-zein in the binding and assembly

34 e alpha- and delta-zeins and the 16-kD gamma-zein and the 15-kD beta-zein; however, the 50- and 27-kD

35 stribution of mRNAs encoding the 22-kD alpha-zein and the 27-kD gamma-zein proteins on cisternal and

36 In this study, zein and zein/carboxymethyl chitosan (CMCS) nanoparticle

37 Zein and zein/CMCS nanoparticles demonstrated similar pr

38 ases are due to the reduction of lysine-poor zeins and a pleiotropic increase in the lysine-rich non-

39 mutant was null for the 27- and 50-kD gamma-zeins and abolished vitreous endosperm formation.

40 ractions among the 50-, 27-, and 16-kD gamma-zeins and the 15-kD beta-zein, consistent with their col

41 s were detected between the alpha- and delta-zeins and the 16-kD gamma-zein and the 15-kD beta-zein;

42 at bound preferentially the alpha- and delta-zeins and the beta- and gamma-zeins.

43 tant endosperm to accumulate carotenoids and zeins and to differentiate aleurone.

44 soybean; TTMPLW, alpha-casein; VHLPP, alpha-zein) and the six alanine substitution peptides of PGTAV

45 s caused by o2, the 22-kDa alpha-zein, gamma-zein, and beta-zein RNAis were stacked, resulting in pro

46 visco-elastic mass softness with kafirin and zein, and for elastic recovery of kafirin.

47 delta-zeins, the 22-kD alpha-zein, the beta-zein, and the gamma-zein RNA interference (RNAi; designa

48 ked with the locus encoding the 16-kDa gamma-zein, and two-dimensional gel electrophoresis confirmed

49 ll gene families encode the gamma- and delta-zeins, and members of these gene families, especially th

50 Whey protein and zein are of nutritional interest due to their high leuci

51 This study showed that zeins are by far the most highly expressed genes in the

52 After being assembled in the ER, zeins are delivered to the aleurone PSVs in atypical pre

53 Although alpha-zeins are encoded by large multigene families, only a fe

54 o2; gammaRNAi/+ genotype suggests that gamma-zeins are essential for restoring protein body density a

55 families, which provides evidence that gamma-zeins are synthesized throughout the endosperm before al

56 The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, w

57 of these gene families, especially the gamma-zeins, are highly expressed.

58 Therefore, the visco-elastic properties of zein arise as a result of non-covalent interactions.

59 Here we attempt to review the literature on zein as a biopolymer for drug/vaccine/gene delivery and

60 In addition, the use of zein as a functional film coating material for new biome

61 This work identifies 27-kD gamma-zein as an opaque2 modifier gene within the largest QPM

62 ng the expression of a subset of 22-kD alpha-zeins, as well as additional endosperm gene functions.

63 hat migrates between the 22- and 19-kD alpha-zein bands.

64 Three zein-based devices are proposed for several applications

65 me incremental gene amplification, the 19-kD zein branch exhibited a greater degree of far-distance g

66 to 3-fold higher levels of the 27-kDa gamma-zein, but the physiological significance of this increas

67 Substantial loss of the 22-kD alpha-zeins by z1CRNAi resulted in protein body budding struct

68 lations indicated that the interaction alpha-zein-Ca(2+) through C-ter was more favorable than Glu48.

69 In addition, the binding constant for the zein-calcium interaction was calculated indicating a hig

70 at manipulating non-covalent interactions in zein can alter and in some cases, completely disrupt the

71 The reduction of zein can be achieved by a transcriptional mutation, opaq

72 In this study, zein and zein/carboxymethyl chitosan (CMCS) nanoparticles were pr

73 s led to the identification of a 19-kD alpha-zein cDNA in which proline replaces serine at the 15th p

74 equence alignments with putative maize delta-zein cis-localization elements identified several candid

75 esult from reduction of only the 22-kD alpha-zein class.

76 Concomitant reduction of all zein classes resulted in severe reduction in protein bod

77 Zein and zein/CMCS nanoparticles demonstrated similar protection

78 Compared with zein nanoparticles, zein/CMCS nanoparticles exhibited better protection of I

79 e encapsulation of hydrophobic bioactives in zein/CMCS nanoparticles is a promising approach to impro

80 application in tablet production, effects of zein coating on tablet properties are still not fully un

81 and is linked with a cluster of 22-kD alpha-zein coding sequences; the other quantitative trait locu

82 7-, and 16-kD gamma-zeins and the 15-kD beta-zein, consistent with their colocalization in developing

83 The zein-containing prevacuolar compartments are neither sur

84 We showed that the 27-kilodalton (kD) gamma-zein controls protein body initiation but is not involve

85 ggests that the localized synthesis of gamma-zeins could initiate and target protein body formation a

86 SDS-PAGE bands did not show any evidence of zein crosslinking.

87 Surface coating of aerogel with zein decreased the oxidation susceptibility of the loade

88 The gamma-zein deletion further increased lysine in QPM in its hom

89 Kernels hemizygous for the gamma-zein deletion had intermediate 27- and 50-kD gamma-zein

90 beta-zein; however, the 50- and 27-kD gamma-zeins did not interact with the alpha- and delta-zein pr

91 Elimination of gamma-zeins disrupts endosperm modification by o2 modifiers, i

92 While the delta-zein double null mutant had negligible effects on protei

93 antioxidant activity after incorporation in zein electrospun fibres.

94 the activation and modulation of the 22-kDa zein-encoding genes.

95 Zera (gamma-Zein ER-accumulating domain) is the N-terminal proline-r

96 rstand the effect of these factors on 22 kDa zein expression, we have cloned one of these and identif

97 nd o2 resulted in further reduction of alpha-zein expression.

98 t in protein volume when calcium is added to zein extracted from nixtamalized flour.

99 Products of the multigene alpha-zein families and the single-gene gamma-zein family are

100 lpha-zein families and the single-gene gamma-zein family are arranged in the central hydrophobic core

101 Conversely, other gamma-zein family members function more in protein body expans

102 istic insights into the relationship between zein film and various improved profiles.

103 or at least 20 days at -20 degrees C, so the zein film can preserve and deliver both the enzyme and s

104 will benefit future prospects of the use of zein film in drug delivery and biomedical applications.

105 s related to the behaviors and properties of zein films are also summarized and analyzed based on pub

106 Zein forms viscoelastic networks in water which have sho

107 sence of ATIs or the control storage protein zein from corn.

108 indicating a higher affinity for calcium by zein from nixtamalized samples.

109 d not reveal differences between patterns of zeins from nixtamalized and control samples.

110 ropic effects caused by o2, the 22-kDa alpha-zein, gamma-zein, and beta-zein RNAis were stacked, resu

111 A188 contains a second copy of the 27-kD zein gene and a 2-kb repetitive element.

112 rence (RNAi) constructs derived from a 22-kD zein gene could produce a dominant opaque phenotype.

113 und that only 27-kDa gamma- and 22-kDa alpha-zein gene expression were affected, whereas the level of

114 bserved, which correlate with an increase in zein gene expression.

115 s analysis also shows that the 22- and 19-kD zein gene families shared a common ancestor.

116 expression of the alpha-, gamma-, and delta-zein gene families, which provides evidence that gamma-z

117 Previously, 41-48 gene copies of the alpha zein gene family that spread over six loci spanning betw

118 et of clones containing members of the 19-kD zein gene family, which previously had been estimated to

119 -distance gene translocations than the 22-kD zein gene family.

120 pplied this system for the enrichment of the zein gene from maize in eight cereal product samples.

121 Here, we describe DNA enrichment of the zein gene from maize using pyrrolidinyl peptide nucleic

122 ated that only cereal samples containing the zein gene from maize yielded positive results, indicatin

123 Each zein gene is contained within a repeat unit that varies

124 mEmBP-1 binds to the O2 box from the 22 kDa zein gene promoter as a homodimer, it is unable to heter

125 proteins that recognize the O2 box in 22 kDa zein gene promoters.

126 MON 810 maize event specific and endogenous zein gene sequences in 1:1 ratio in tandem was construct

127 only 20bp upstream of the alpha-class 22-kDa zein gene-specific cis element, the O2-box, which is rec

128 ying a TaqMan RT-PCR targeting the P-35S and zein gene.

129 Affinities between zeins generally were consistent with results from immuno

130 n inverse relationship between the number of zein genes and the relative amount of specific mRNAs.

131 Because the 27- and 16-kDa gamma-zein genes are highly conserved in DNA sequence, we intr

132 deletions in intergenic regions, many of the zein genes are spaced over different distances.

133 quences and linear organization of the 19-kD zein genes in maize (Zea mays).

134 r long sequence of a cluster of 22-kDa alpha zein genes in the maize inbred BSSS53 was determined.

135 eletion encompassing the 27- and 50-kD gamma-zein genes on chromosome 7 and a deletion of at least 23

136 ve clones containing the entire set of 19-kD zein genes were chosen from each region and sequenced.

137 nse RNA also reduced the expression of 22-kD zein genes, but failed to give an opaque phenotype.

138 provide the linear organization of 25 19-kD zein genes, one-half the number previously estimated.

139 30 mutation maps in a cluster of 19-kD alpha-zein genes, we characterized cDNA clones encoding these

140 hionine to 69.0 +/- 5.8% for arginine in the zein group.

141 in the absence of the aeroallergen, whereas zein had no such effect.

142 alterations, indicating that beta- and gamma-zeins have redundant and unique functions in the stabili

143 Studies, mostly with zein, have demonstrated the potential of using plant pro

144 and the 16-kD gamma-zein and the 15-kD beta-zein; however, the 50- and 27-kD gamma-zeins did not int

145 Furthermore, we developed a pectin/zein hydrogel bead system to specifically deliver p40 to

146 fter administration of p40-containing pectin/zein hydrogel beads to mice.

147 g quercetin (water insoluble polyphenol) and zein (hydrophobic protein), simultaneously, by adding th

148 the absence of null mutants of each type of zein (i.e. alpha, beta, gamma, and delta), the molecular

149 estibility of whey protein isolate (WPI) and zein in healthy volunteers by use of the naso-ileal intu

150 for the 16-kD gamma-zein and the 15-kD beta-zein in the binding and assembly of alpha-zeins within t

151 In this review, the present status of zein in the form of a thin film and uniform layer for us

152 ins, the homologues of the maize 22-kD alpha-zeins in sorghum (Sorghum bicolor), in the beta/gammaRNA

153 in bodies ectopically accumulate 22-kD alpha-zeins in the gamma-zein-rich periphery and center of the

154 As encoding alpha-, beta-, gamma-, and delta-zeins in the yeast two-hybrid system.

155 Zeins in W64A o5, o9, o11, and Mc are within 80 to 90% o

156 rticles with a layer of hydrophobic protein (zein) increased stability and further decreased the reac

157 e the wild-type protein, the Mc 16-kDa gamma-zein interacted only weakly with the 22-kDa alpha-zein w

158 ibre content, the calcium-starch and calcium-zein interactions, as well as the presence of amylose-li

159 roper deposition of storage proteins, called zeins, into specialized organelles in the endosperm, cal

160 Zein is a class of alcohol-soluble prolamine proteins pr

161 s in healthy humans and, in contrast to WPI, zein is poorly digestible.

162 rophobic interactions while the binding with zein is predominantly mediated through hydrogen bonds.

163 In maize, gamma-Zein is the major storage protein synthesized by the rou

164 The 19-kD alpha-zein is uniformly distributed throughout the core in wil

165 cating that a sufficient amount of the 22-kD zeins is necessary for maintenance of a normal protein b

166 A biodegradable material, zein, is proposed as a reagent delivery platform for bio

167 eletion had intermediate 27- and 50-kD gamma-zein levels and were semivitreous, indicating haploinsuf

168 zed delta-zein was probed by comparing delta-zein levels of starch granules obtained from homogenized

169 previously reported for kernels with reduced zein levels.

170 A new allele of the 27-kD zein locus in maize has been generated by interchromosom

171 Allelic variation at the 22-kD alpha-zein locus may contribute to the difference of eEF1A con

172 ne of the QTLs is linked to the 27-kDa gamma-zein locus on chromosome 7S.

173 two progenitors of maize gained a new alpha zein locus, absent in the other lineage, to form a nondu

174 perties of visco-elastic materials made from zein, making them softer and more extensible, as did ure

175 maize plants expressing the Mc 16-kDa gamma-zein manifested an opaque kernel phenotype with enhanced

176 Zein masses exhibited predominantly viscous flow propert

177 es have a much higher elastic character than zein masses.

178 fferent degrees of gene amplification in the zein multigene family.

179 protein-free biscuits and a drink containing zein (n = 8), WPI (n = 7), or no protein (protein free,

180 Chitosan-Zein Nano-in-Microparticles (CS-ZN-NIMs), consisting of

181 lusion complex (IC) encapsulated electrospun zein nanofibrous webs (zein-THY/gamma-CD-IC-NF) were fab

182 icle pesticide delivery vehicle to soybeans, zein nanoparticle (ZNP) uptake by the roots and biodistr

183 etermination of the actual lutein content in zein nanoparticles using ultraviolet-visible spectroscop

184 After zein nanoparticles were coated with CMCS, the zeta poten

185 Compared with zein nanoparticles, zein/CMCS nanoparticles exhibited be

186 ion was able to improve the functionality of zein networks for the purpose of food structuring, and a

187 estigates an alternative method of preparing zein networks through antisolvent precipitation, involvi

188 ysical properties of brittle, self-assembled zein networks through microbial transglutaminase crossli

189 rheological and structural properties of the zein networks were analyzed and determined that modifica

190 Zeins of differing sub-class composition much more readi

191 PI intake (P = 0.0319), whereas no effect of zein on aminoacidemia was observed, including plasma leu

192 1.03 (histidine) for WPI and close to 0 for zein, owing to its negligible lysine content.

193 in presenting clear demonstration that gamma-zeins play a mechanistic role in QPM, providing a previo

194 th earlier studies that suggested that gamma-zeins play an important role in prolamin protein body as

195 s of water or acetic acid treatment, all the zein preparations had similar FTIR spectra, with greater

196 sis revealed that the enzymatic treatment of zein produced a weaker, more brittle structure.

197 ments and double mutant kernels had restored zein profiles.

198 ier demonstrated that the RNAs for the maize zeins ('prolamine' class) are localized to the spherical

199 nhibited regulated transcription of a 22 kDa zein promoter in a transient expression assay using cult

200 mechanism seems to apply to the 27-kDa gamma-zein promoter, which does not undergo methylation change

201 This gene was regulated by the 27-kD gamma-zein promoter, which restricted synthesis of the defecti

202 but at a lower affinity than to the '27-kDa' zein promoter.

203 th the expression of endogenous 22-kDa alpha-zein promoters, a different mechanism seems to apply to

204 bind to the P-box from '22-kDa' and '19-kDa' zein promoters, but at a lower affinity than to the '27-

205 rom needle-like to spherical shape at higher zein proportions, as confirmed by transmission electron

206 Zein protein bodies in fl1 mutants are of normal size, s

207 Zein protein bodies in Mc endosperm are misshapen and ar

208 have an opaque, starchy phenotype, malformed zein protein bodies, and highly increased levels of bind

209 rt the cloning of Fl1, which encodes a novel zein protein body membrane protein with three predicted

210 Immunoblotting revealed a novel alpha-zein protein in De*-B30 that migrates between the 22- an

211 This finding implies that zein protein interactions determine protein body assembl

212 l electrophoresis confirmed the 16-kDa gamma-zein protein is altered in Mc.

213 The largest reductions in zein protein synthesis occur in the W64A o2, DeB30, and

214 tation in the gene encoding the 16-kDa gamma-zein protein, leading to the unfolded protein response i

215 attributed mainly to the contribution of the zein protein.

216 ury mutant based on a misfolded 16-kDa gamma-zein protein.

217 rant RNA in Mc that encodes the 16-kDa gamma-zein protein.

218 a defective signal peptide in a 19-kD alpha-zein protein.

219 engineering, kernels with reduced levels of zein proteins have been shown to have increased levels o

220 ypothesis that a pleiotropic increase in non-zein proteins is contributing to an improved amino acid

221 ing the 22-kD alpha-zein and the 27-kD gamma-zein proteins on cisternal and protein body rough endopl

222 f hydrophobic interactions within individual zein proteins or interactions between proteins.

223 increase of more nutritionally balanced non-zein proteins, and therefore enhance the overall quality

224 pleiotropic increase in the lysine-rich non-zein proteins.

225 without affecting the accumulation of other zein proteins.

226 s did not interact with the alpha- and delta-zein proteins.

227 te to the accumulation of normal-size 22-kDa zein proteins.

228 The average particle size of zein:quercetin composite particles was below 200 nm (130

229 Both zein reduced kernels contained higher levels of lysine a

230 of developing kernels of these two types of zein reduced kernels were compared.

231 sible for the increased lysine in transgenic zein reduction (TZR) kernels.

232 aspartate and glutamate, was observed in the zein reduction kernels.

233 Zein reduction results in an increase of more nutritiona

234 Only three out of 10 zein-related sequences have an intact open reading frame

235 sts contained markedly lower levels of delta-zein relative to granules prepared from whole endosperm,

236 on were affected, whereas the level of other zeins remained unchanged.

237 ly accumulate 22-kD alpha-zeins in the gamma-zein-rich periphery and center of the core, rather than

238 Aleurone PSVs contain zein-rich protein inclusions, a matrix, and a large syst

239 -kD alpha-zein, the beta-zein, and the gamma-zein RNA interference (RNAi; designated as z1CRNAi, beta

240 s the PB-ER localization of the 10-kDa delta-zein RNA is maintained in developing rice seeds, we dete

241 by expressing GFP fusions containing various zein RNA sequences in transgenic rice and analyzing thei

242 A gamma-zein RNAi transgene was able to rescue the mutation and

243 the 22-kDa alpha-zein, gamma-zein, and beta-zein RNAis were stacked, resulting in protein bodies for

244 protein denaturation and disulfide bonds on zein's ability to form a visco-elastic material.

245 reducing agent beta-ME had little effect on zein's ability to form a visco-elastic material.

246 Immunolocalization of the 50-kD gamma-zein showed this protein to be located at the surface of

247 causes translational recoding of lysine into zeins, significantly enriching the lysine content of gra

248 mays L.) opaque kernel mutation that alters zein storage protein synthesis.

249 mays) endosperm by reducing the synthesis of zein storage proteins and increasing the accumulation of

250 oper formation of protein bodies (PBs) where zein storage proteins are deposited.

251 ent in corn meal because of the abundance of zein storage proteins that lack these amino acids.

252 rin were found in addition to the endogenous zein storage proteins, demonstrating that the large exog

253 g a starchy endosperm with reduced levels of zein storage proteins, homozygous zmsmu2-1 mutants manif

254 ed RNA interference suppression of different zein subclasses to abolish vitreous endosperm formation

255 Reduction in both 19- and 22-kD alpha-zein subfamilies severely restricted protein body expans

256 Novel biomedical applications of zein such as controlled and targeted delivery of bioacti

257 ased by the opaque-2 mutation, which reduces zein synthesis and increases accumulation of proteins th

258 mulates at a high level during the period of zein synthesis and protein body development and declines

259 ant maize (Zea mays) mutation that depresses zein synthesis in the developing endosperm.

260 d Mc were significant qualitative changes in zein synthesis observed.

261 enic (CaMV 35S promoter) and taxon-specific (zein) target DNA sequences.

262 ins, but the binding constant was higher for zein than for gliadin at 35 degrees C.

263 We identified domains within the 22-kD alpha-zein that bound preferentially the alpha- and delta-zein

264 the N-terminal proline-rich domain of gamma-zein that is sufficient to induce the assembly of PB for

265 mutant for the delta-zeins, the 22-kD alpha-zein, the beta-zein, and the gamma-zein RNA interference

266 Here, a double null mutant for the delta-zeins, the 22-kD alpha-zein, the beta-zein, and the gamm

267 In maize, alpha-zeins, the main protein components of seed stores, are m

268 Zeins, the maize (Zea mays) prolamin storage proteins, a

269 Zeins, the prolamin storage proteins found in maize (Zea

270 e time, confirming that aggregation of alpha-zein through calcium bridges is possible, expanding the

271 tion, opaque2 (o2), or a transgene targeting zein through RNA interference (RNAi).

272 ased from zein-THY/gamma-CD-IC-NF (2:1) than zein-THY-NF and zein-THY/gamma-CD-IC-NF (1:1).

273 ein-THY/gamma-CD-IC-NF (2:1) was higher than zein-THY-NF and zein-THY/gamma-CD-IC-NF (1:1).

274 HY/gamma-CD-IC-NF (2:1) than zein-THY-NF and zein-THY/gamma-CD-IC-NF (1:1).

275 -IC-NF (2:1) was higher than zein-THY-NF and zein-THY/gamma-CD-IC-NF (1:1).

276 It is worth mentioning that zein-THY/gamma-CD-IC-NF (2:1) preserved much more THY as

277 Therefore, much more THY was released from zein-THY/gamma-CD-IC-NF (2:1) than zein-THY-NF and zein-

278 Similarly, antibacterial activity of zein-THY/gamma-CD-IC-NF (2:1) was higher than zein-THY-N

279 It was demonstrated that zein-THY/gamma-CD-IC-NF (2:1) was most effective in inhi

280 apsulated electrospun zein nanofibrous webs (zein-THY/gamma-CD-IC-NF) were fabricated as a food packa

281 nd Na2SO4 negatively impacted the ability of zein to from a visco-elastic material and at higher conc

282 llic acid was successfully incorporated into zein ultra-fine fibres at different loading amount (5%,

283 and the interaction between gallic acid and zein was attested by attenuated total reflection-Fourier

284 from this cross, and a higher level of alpha-zein was found to cosegregate with high eEF1A content.

285 the formation of visco-elastic material from zein was investigated.

286 True ileal nitrogen digestibility of zein was markedly lower than WPI (60.2 +/- 4.5% and 91.2

287 The content of alpha-zein and gamma-zein was measured in pools of high- and low-eEF1A indivi

288 The origin of surface-localized delta-zein was probed by comparing delta-zein levels of starch

289 Zein was tagged with fluorescein isothiocyanate (FITC) a

290 rticles or other fluorescent contaminants of zein were up taken by the roots and biodistributed withi

291 Interactions between the 19- and 22-kD alpha-zeins were relatively weak, although each of them intera

292 interacted only weakly with the 22-kDa alpha-zein when expressed in the yeast two-hybrid system.

293 thermolysin led to selective removal of the zeins, whereas granule-associated proteins of 32 kD or a

294 the accumulation of both 19- and 22-kD alpha-zeins, which resulted in higher lysine and tryptophan co

295 The spatial organization of zeins within the protein body, as well as interactions b

296 ta-zein in the binding and assembly of alpha-zeins within the protein body.

297 ormal Mendelian fashion and eliminates 22-kD zeins without affecting the accumulation of other zein p

298 sequenced all 23 members of the 22-kD alpha zein (z1C) gene family of maize.

299 The applicability of gallic acid loaded zein (Ze-GA) electrospun fibre mats towards potential ac

300 stem consisting of two natural biomaterials, zein (ZN) and chitosan (CS), to mediate oral DNA deliver