ライフサイエンスコーパス: zinc finger nuclease

1 ough linked protein domains (e.g. TALENs and zinc-finger nucleases).

2 th activity on par with a homodimeric Zif268 zinc-finger nuclease.

3 we compared with the well-established 'QQR' zinc-finger nuclease.

4 ogenous Ig loci were silenced using designer zinc finger nucleases.

5 on of alpha or beta TCR chains with designer zinc finger nucleases.

6 integrating RNA destabilizing elements using zinc finger nucleases.

7 a cells by targeted mutation of exon 8 using zinc finger nucleases.

8 t HIV Rev protein M10, fusion inhibitors and zinc-finger nucleases.

9 ation can now be reliably accomplished using zinc-finger nucleases.

10 may be applied to correction of X-CGD using zinc finger nuclease and patient iPSCs.

11 Finally, gene editing techniques such as zinc finger nucleases and CRISPR aim to correct genetic

12 To this end, we developed designer zinc finger nucleases and employed a "hit-and-run" appro

13 er other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their e

14 to other customizable endonucleases, such as zinc finger nucleases and transcription activator-like e

15 efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like e

16 Tev-LHEs are distinct from the FokI-derived zinc-finger nuclease and TAL effector nuclease platforms

17 of the nuclear genome by approaches such as zinc-finger nucleases and homologous recombination.

18 ed the Plasmodium falciparum K13 locus using zinc-finger nucleases and measured ring-stage survival r

19 ccurring nucleases, including meganucleases, zinc finger nucleases, and transcription activator-like

20 porter gene carried a recognition site for a zinc-finger nuclease, and protoplasts from each tobacco

21 equency and spectrum of restriction-enzyme-, zinc-finger-nuclease-, and RAG-induced DSBs.

22 for the wild-type allele were edited using a Zinc Finger Nuclease approach.

23 Zinc finger nucleases are artificial restriction enzymes

24 Zinc-finger nucleases are chimeric proteins consisting o

25 codons and exerting control by microRNAs or zinc finger nucleases--are providing new ways of control

26 g DSBs (5' DSBs) in yeast using an optimized zinc finger nuclease at an efficiency that approached HO

27 Zinc finger nucleases can be designed to stimulate homol

28 Furthermore, we demonstrate that zinc finger nucleases can eliminate HLA-A expression fro

29 Engineered zinc finger nucleases can stimulate gene targeting at sp

30 double-strand break induced by the resulting zinc-finger nuclease can create specific sequence altera

31 The DNA-binding domain encoded by zinc-finger nucleases can be engineered to recognize a v

32 n vivo applications and to determine whether zinc finger nucleases create undesired genomic instabili

33 We show that zinc-finger nucleases designed against an X-linked sever

34 The clrn1 mutants generated by zinc finger nucleases displayed aberrant hair bundle mor

35 rat expressing the G551D variant obtained by zinc finger nuclease editing of a human complementary DN

36 Although zinc-finger nucleases enable a variety of genome modific

37 assist selection of rare targeted clones and zinc finger nucleases engineered to specifically stimula

38 ial fingers can be used to create functional zinc finger nucleases for editing vertebrate genomes.

39 ising the possibility of strategies based on zinc-finger nucleases for the treatment of disease.

40 Here, we demonstrate that engineered zinc-finger nucleases function effectively in somatic ce

41 ctivator-like effector protein nuclease, and zinc finger nuclease gene editing strategies to either d

42 emonstrate that chromosome breaks created by zinc-finger nucleases greatly enhance the frequency of l

43 Zinc finger nuclease in-embryo editing of the RELA locus

44 Gene targeting by zinc-finger nucleases in one-cell embryos provides an ex

45 Here we apply zinc-finger nucleases in one-cell mouse embryos to gener

46 as an anticancer strategy, we disrupted, via zinc finger nucleases, MCT4 and BASIGIN genes in colon a

47 n T1D, we targeted Ubash3a in NOD mice using zinc-finger nuclease mediated mutagenesis.

48 In this work, using zinc finger nuclease-mediated gene editing, we have sele

49 d mitochondrial DNA (mtDNA) damage and after zinc finger nuclease-mediated gene mutation correction,

50 Zinc finger nuclease-mediated gene targeting of a single

51 ntal requirement for Rest in zebrafish using zinc-finger nuclease-mediated gene targeting.

52 Zinc-finger nuclease-mediated targeted gene deletion of

53 ted a Go-opsin1 knockout Platynereis line by zinc-finger-nuclease-mediated genome engineering.

54 The zinc finger nuclease method was used to establish rat li

55 as motor and behavioural function in a novel zinc-finger nuclease model of RTT utilizing both male an

56 By combining zinc finger nuclease mRNA delivery with AAV6 delivery of

57 strate that temporally optimized delivery of zinc finger nuclease mRNA via electroporation and adeno-

58 Use of the mitochondrially targeted zinc finger-nuclease (mtZFN) results in degradation of m

59 ns and in cells genetically engineered using zinc-finger nucleases, single-nucleotide changes occur f

60 ry and development of DNA-editing nucleases (Zinc Finger Nucleases, TALENs, CRISPR/Cas systems) has g

61 s this, gene-editing technologies, including zinc-finger nucleases, TALENs, CRISPR-Cas, base editing,

62 ines made deficient in O-glycan extension by zinc finger nuclease targeting of a key gene in O-glycan

63 hrombin III gene (at3) in zebrafish by using zinc finger nuclease technology.

64 mutations into the zebrafish fga gene using zinc finger nuclease technology.

65 developed a novel Bace1(-/-) rat line using zinc-finger nuclease technology and compared Bace1(-/-)

66 Using zinc-finger nuclease technology to engineer an integrati

67 Using the zinc-finger nuclease technology, the generation of a tar

68 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drast

69 Dahl salt-sensitive (SS/JrHsdMcwi) rat using zinc-finger nuclease technology.

70 We have used this system to identify zinc-finger nucleases that exhibit high cleavage activit

71 are other potential biotechnologies, such as zinc finger nucleases, that could be also used for trans

72 In addition, we used Zinc finger nucleases to generate isogenic SHANK3 knocko

73 We used zinc finger nucleases to induce stable expression of hum

74 We have used zinc-finger nucleases to drive the simultaneous genetic

75 Here we use zinc-finger nucleases to generate targeted mutations in

76 Here we use zinc-finger nucleases to genetically modify the multidru

77 Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we report

78 ogies for facile manipulation of the genome (zinc finger nucleases, transcription activator-like effe

79 Gene editing with zinc finger nucleases, transcription activator-like effe

80 e mainstream genome editing tools, including zinc finger nucleases, transcription activator-like effe

81 Zinc-finger nuclease, transcription activator-like effec

82 ologies are rapidly evolving, from the early zinc-finger nucleases, transcription activator-like effe

83 t expression of sequence-specific nucleases (zinc-finger nucleases, transcription activator-like effe

84 go, first with meganucleases and followed by zinc finger nucleases, transcriptional activator-like ef

85 Here we develop a zinc-finger nuclease translocation reporter and screen f

86 genome editing proteins, Cre recombinase and zinc-finger nucleases, under conditions in which (-30)GF

87 Disruption of CCR5 using zinc finger nucleases was the first-in-human application

88 Using genome editing with zinc finger nucleases, we inserted a large, inducible XI

89 Using zinc finger nucleases, we show that disruption of the ze

90 Three-finger zinc finger nucleases were designed based on previously

91 To study the protein in more detail, zinc finger nucleases were used to edit the PORCN genomi

92 o wild-type (MSTN(+/+) ) rats, rats in which zinc finger nucleases were used to genetically inactivat

93 ome of the Dahl Salt-sensitive (S) rat using zinc-finger nucleases, wherein the mutant rat had a 17 b

94 ccelerated site-specific DNA cleavage by the zinc-finger nuclease, without enhancing off-target cleav

95 ement therapies that addresses these issues: zinc finger nuclease (ZFN) -mediated site-specific integ

96 activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can be engineered into site-s

97 A transgene, flanked by zinc finger nuclease (ZFN) cleavage sites, was deleted f

98 activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology ena

99 The zinc finger nuclease (ZFN) mediated genomic editing gene

100 we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with donor template deli

101 The emerging zinc finger nuclease (ZFN) technology facilitates gene t

102 The development of zinc finger nuclease (ZFN) technology has enabled the ge

103 s to develop RNA-based approaches to deliver zinc finger nuclease (ZFN), evaluate the effect of CCR5

104 FP respectively) by targeted integration via zinc finger nuclease (ZFN)--mediated homologous recombin

105 Initially, we injected zinc finger nuclease (ZFN)-encoding mRNA or DNA into bov

106 past two years, advances in transposon- and zinc finger nuclease (ZFN)-mediated gene knockout as wel

107 By combining zinc finger nuclease (ZFN)-mediated genome editing and i

108 ate a method for ligand-mediated delivery of zinc finger nucleases (ZFN) proteins using transferrin r

109 -mutant Meishan pigs with no marker gene via zinc finger nucleases (ZFN) technology.

110 are genetically modified with mRNA encoding zinc finger nucleases (ZFN) that target and disrupt a sp

111 ryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcription activator-l

112 liver-targeted in vivo genome editing using zinc-finger nuclease (ZFN) technology to insert the huma

113 This method uses a zinc-finger nuclease (ZFN) to create a site-specific dou

114 was rendered permanently dysfunctional by a zinc-finger nuclease (ZFN)--is safe.

115 Zinc-finger nuclease (ZFN)-based in vivo genome editing

116 three genes in human pluripotent cells using zinc-finger nuclease (ZFN)-mediated genome editing.

117 The technology of zinc finger nucleases (ZFNs) allows DNA double-strand br

118 Targeted endonucleases including zinc finger nucleases (ZFNs) and clustered regularly int

119 no-associated virus (AAV) vector delivery of zinc finger nucleases (ZFNs) and corrective donor templa

120 vectors by using a combination of engineered zinc finger nucleases (ZFNs) and homing endonucleases.

121 Here we show that a combination of zinc finger nucleases (ZFNs) and piggyBac technology in

122 ering sequence-specific nucleases, including zinc finger nucleases (ZFNs) and TAL effector nucleases

123 This method is applicable with both zinc finger nucleases (ZFNs) and Tale nucleases (TALENs)

124 ablished a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator

125 Zinc finger nucleases (ZFNs) and transcription activator

126 Zinc finger nucleases (ZFNs) are engineered restriction

127 Zinc finger nucleases (ZFNs) are hybrid proteins that ha

128 Zinc finger nucleases (ZFNs) are powerful tools for gene

129 Here it is reported that designed zinc finger nucleases (ZFNs) can drive site-directed DNA

130 ed double-strand break induced by engineered zinc finger nucleases (ZFNs) can stimulate integration o

131 Zinc finger nucleases (ZFNs) facilitate tailor-made geno

132 We describe the use of designed zinc finger nucleases (ZFNs) for efficient transgenesis

133 on of genomic double-strand breaks (DSBs) by zinc finger nucleases (ZFNs) has been deployed for gene

134 The development of zinc finger nucleases (ZFNs) has permitted efficient gen

135 Engineered zinc finger nucleases (ZFNs) induce DNA double-strand br

136 A pair of zinc finger nucleases (ZFNs) or transcription activator-

137 Zinc finger nucleases (ZFNs) show promise in improving t

138 The first truly targetable reagents were the zinc finger nucleases (ZFNs) showing that arbitrary DNA

139 off-target site for the extensively studied zinc finger nucleases (ZFNs) targeting C-C chemokine rec

140 an approach for improving the specificity of zinc finger nucleases (ZFNs) that engineers the FokI cat

141 stability in mammalian systems, we developed zinc finger nucleases (ZFNs) that recognize and cleave C

142 by the transient expression of CCR5-targeted zinc finger nucleases (ZFNs) to generate CCR5-negative c

143 5 (CCR5) locus in pigtailed macaque HSPCs by zinc finger nucleases (ZFNs) was feasible.

144 ds for identifying the off-target effects of zinc finger nucleases (ZFNs) were described-one using an

145 Novel zinc finger nucleases (ZFNs) were designed to target the

146 Custom-designed zinc finger nucleases (ZFNs), proteins designed to cut a

147 n or correction, based on the development of zinc finger nucleases (ZFNs), transcription activator-li

148 iew, we introduce the three central methods, zinc finger nucleases (ZFNs), transcription activator-li

149 Zinc finger nucleases (ZFNs), which are nucleases conjug

150 dopsis genes through regulated expression of zinc finger nucleases (ZFNs)-enzymes engineered to creat

151 Here we report on the use of zinc finger nucleases (ZFNs)-hybrid synthetic restrictio

152 nduction of site-specific genomic DSBs using zinc finger nucleases (ZFNs).

153 Zinc-finger nucleases (ZFNs) allow targeted gene inactiv

154 relies on cleavage of the target by designed zinc-finger nucleases (ZFNs) and production of a linear

155 While the specificity of zinc-finger nucleases (ZFNs) and RNA-guided endonuclease

156 Zinc-finger nucleases (ZFNs) and TAL effector nucleases

157 This study optimized zinc-finger nucleases (ZFNs) and transcription activator

158 of engineered site-directed nucleases, like zinc-finger nucleases (ZFNs) and transcription activator

159 Zinc-finger nucleases (ZFNs) and transcription activator

160 Zinc-finger nucleases (ZFNs) are hybrids between a nonsp

161 Zinc-finger nucleases (ZFNs) are important tools for gen

162 Zinc-finger nucleases (ZFNs) are powerful tools for edit

163 Engineered zinc-finger nucleases (ZFNs) are promising tools for gen

164 Zinc-finger nucleases (ZFNs) are targetable DNA cleavage

165 Zinc-finger nucleases (ZFNs) are versatile reagents that

166 Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome

167 ly, we and others have shown that customized zinc-finger nucleases (ZFNs) can introduce targeted fram

168 Using zinc-finger nucleases (ZFNs) designed to flank the sickl

169 By using engineered zinc-finger nucleases (ZFNs) designed to target an integ

170 Zinc-finger nucleases (ZFNs) drive efficient genome edit

171 Zinc-finger nucleases (ZFNs) drive highly efficient geno

172 Engineered zinc-finger nucleases (ZFNs) enable targeted genome modi

173 The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is h

174 We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline di

175 Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly

176 Zinc-finger nucleases (ZFNs) have been used for genome e

177 Zinc-finger nucleases (ZFNs) have emerged as powerful to

178 Zinc-finger nucleases (ZFNs) have enabled highly efficie

179 Recently, microinjection of engineered zinc-finger nucleases (ZFNs) in embryos was used to gene

180 specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs.

181 olution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-strand

182 d genetic engineering of this parasite using zinc-finger nucleases (ZFNs) that produce a double-stran

183 We designed zinc-finger nucleases (ZFNs) that promoted the disruptio

184 Using zinc-finger nucleases (ZFNs) to cleave the chromosomal t

185 -resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5.

186 We tested the feasibility of using zinc-finger nucleases (ZFNs) to knock out a gene directl

187 Genome editing driven by zinc-finger nucleases (ZFNs) yields high gene-modificati

188 gene-targeting approach in the monarch using zinc-finger nucleases (ZFNs), engineered nucleases that

189 s strains (including wild-type CC-125) using zinc-finger nucleases (ZFNs), genetically encoded CRISPR

190 Designer nucleases, like zinc-finger nucleases (ZFNs), represent valuable tools f

191 uding homing endonucleases or meganucleases, zinc-finger nucleases (ZFNs), TAL effector nucleases (TA

192 From the pioneering work using zinc-finger nucleases (ZFNs), to the advent of the versa

193 Targeted nucleases, including zinc-finger nucleases (ZFNs), transcription activator-li

194 ree classes of targetable cleavage reagents: zinc-finger nucleases (ZFNs), transcription activator-li

195 Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human

196 to enable such high-precision targeting with zinc-finger nucleases (ZFNs), we have developed an expan

197 rgeting in mice using embryonic injection of zinc-finger nucleases (ZFNs), which generate site-specif

198 To stably modify this organism, we used zinc-finger nucleases (ZFNs), which take advantage of ho

199 Zinc-finger nucleases (ZFNs)-enzymes engineered to creat

200 address this problem by genome editing with zinc-finger nucleases (ZFNs).

201 with similar efficiency and precision as do zinc-finger nucleases (ZFNs).

202 endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs).

203 ctivator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).

204 embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs).

205 nockout in mammalian cells, using engineered zinc-finger nucleases (ZFNs).

206 t a specific site have been introduced using zinc-finger nucleases (ZFNs).

207 of a targeted-mutagenesis approach based on zinc-finger nucleases (ZFNs).