ライフサイエンスコーパス: zymography

1 o cell surfaces was quantified using in vivo zymography.

2 ochemistry, quantitative RT-PCR, and in situ zymography.

3 ty levels of MMP-2 and -9 were determined by zymography.

4 by using MMP immunostaining and in situ MMP zymography.

5 termined by immunohistochemistry and gelatin zymography.

6 by RT-PCR, Western blot, activity assay, and zymography.

7 MMP-3 activity was not detected on zymography.

8 y quantitative reverse transcription-PCR and zymography.

9 nitude lower than what has been reported for zymography.

10 tivity of amebic proteinases was examined by zymography.

11 yzed for MMP-2 and -9 activities by SDS-PAGE zymography.

12 MMP-2 by RT-qPCR, Western blot analysis, or zymography.

13 l fluid (SF) from OA patients is analyzed by zymography.

14 and TIMP activity was determined by reverse zymography.

15 oteinase-9 (MMP-9) were evaluated by in situ zymography.

16 activity were determined by Western blot and zymography.

17 activity in OA SF was determined by gelatin zymography.

18 d media were determined by ELISA and gelatin zymography.

19 MMP-2 activity was analyzed by gelatin zymography.

20 d gelatinolytic activity detected by in situ zymography.

21 d conditioned media were analyzed by gelatin zymography.

22 ones that cannot be analyzed by traditional zymography.

23 and protease activity was assessed by casein zymography.

24 ytic activity in the limb as measured by gel zymography.

25 l protein extracts was determined by gelatin zymography.

26 nd tissue inhibitor of MMP (TIMP) by reverse zymography.

27 trains PA103 and ATCC 19660 were analyzed by zymography.

28 -2 and -9 activity was determined by gelatin zymography.

29 f MMP-2 released from cells were examined by zymography.

30 merase chain reaction, Western blotting, and zymography.

31 ea and conjunctiva was determined by in situ zymography.

32 and TIMPs were analyzed by Western blot and zymography.

33 s assessed in tissue culture supernatants by zymography.

34 stern blot analysis, colorimetric assay, and zymography.

35 conditioned media as demonstrated by gelatin zymography.

36 SA, and MMP-3 activity was assayed by casein zymography.

37 MP-3/4 as assessed by zymography and reverse zymography.

38 Collagenolytic activity was examined by zymography.

39 talloproteinase-9 activity in their lungs on zymography.

40 scription PCR, Western blotting, and gelatin zymography.

41 ed from the dispersion assay was assessed by zymography.

42 teinase (MMP)-2 and -9 was quantified by gel zymography.

43 and tear matrix metalloproteinase (MMP)-9 by zymography.

44 MMP-3 activity was assayed by casein zymography.

45 mogenic peptide assay and plasminogen-linked zymography.

46 predominantly MMP-9 (92 kDa GLSE) by gelatin zymography.

47 by immunofluorescence microscopy and gelatin zymography.

48 inolytic activity as demonstrated by in situ zymography.

49 tors, using a fluorometric assay and gelatin zymography.

50 inase (MMP)-2 and -9 was assessed by gelatin zymography.

51 li were found to display amidase activity by zymography.

52 by Western blotting and immunoprecipitation-zymography.

53 s determined by Western blotting and gelatin zymography.

54 ed MMP-2 enzymatic activity as determined by zymography.

55 be detected at a level of 0.2 ng in gelatin zymography.

56 low levels in conventional casein or gelatin zymography.

57 red on type I collagen films, as assessed by zymography.

58 oteinase (MMP) activities were determined by zymography.

59 y RT-PCR and secretion by ELISA, luminex, or zymography.

60 nzyme-linked immunosorbent assay (ELISA) and zymography.

61 tion dependent manner as measured by gelatin zymography.

62 media were used for immunoblot analyses and zymography.

63 ccording to SDS-PAGE, shown a single band in zymography.

64 ive real-time polymerase chain reaction, and zymography.

65 Only MMP-9 activity was decreased on zymography.

66 thod was similar to the results of substrate zymography.

67 ch the results are consistent with substrate zymography.

68 MMP1 activity was detected using collagen zymography.

69 eaction (qPCR), western blotting, and casein zymography.

70 eal-time PCR; activity of MMP-2/9 by gelatin zymography.

71 y of the inhibitors as observed in substrate zymography.

72 sis, and performed leakage tests and in situ zymography.

73 ression by real-time PCR, and MMP release by zymography.

74 -1 protein levels were assessed by ELISA and zymography.

75 nzyme-linked immunosorbent assay and gelatin zymography.

76 n and its MMP inhibitory activity by reverse zymography.

77 MMP-9 activity was analyzed by zymography.

78 IMP-3-deficient mice was examined by in situ zymography.

79 determined by quantitative real-time PCR and zymography.

80 psin K to femtomole resolution using gelatin zymography.

81 n the hybrid layers was examined via in situ zymography after 24-h storage or after thermomechanical

82 omes were incubated at 37 degrees C prior to zymography, an intense band of proteolytic activity deve

83 Zymography analysis showed antibody treatment decreased

84 Zymography analysis, performed in muscularis whole mount

85 48 hours to evaluate the MMP-9 production by zymography and activity assay.

86 y, behavioural tests, western blotting, MMP9 zymography and analysis of angioneurogenesis were perfor

87 ucted, and their activities were analyzed by zymography and by expressing them in both Escherichia co

88 Gelatinolytic activity of MMP-9 and MMP-2 by zymography and cellular localization by immunohistochemi

89 ity within the HL was examined using in situ zymography and confocal laser scanning microscopy after

90 alloproteinase-2 (MMP-2) activity by gelatin zymography and diminished MMP-2 transcription of a minim

91 Zymography, reverse zymography and ELISA (enzyme-linked immunosorbent assay)

92 ere collected after 24 hours of exposure for zymography and ELISA.

93 tissue homogenates and quantified by gelatin zymography and ELISA.

94 Gelatin zymography and enzyme activity assays performed on prote

95 rotease detection systems, including gelatin zymography and enzyme linked immunosorbent assay (ELISA)

96 mal fibroblasts, as measured by both gelatin zymography and enzyme-linked immunosorbent assay (P < 0.

97 crease in MMP-1 with CTGF only (indicated by zymography and enzyme-linked immunosorbent assay).

98 In addition, our gelatinase zymography and fluorescence data confirmed that the card

99 ctivator (uPA) activities were determined by zymography and fluorogenic assays.

100 ayed for atent and active MMP-2 and MMP-9 by zymography and immunochemistry.

101 Gel electrophoresis zymography and immunofluorescence co-localization sugges

102 addition, sections were studied with in situ zymography and immunohistochemistry staining for MMP-9.

103 teinase-2 activity was quantified by gelatin zymography and immunoprecipitation.

104 detected at a level of 30 pg in transferrin zymography and MMP-1 and -13 can be detected at a level

105 ltured human RPE to assess MMP-2 activity by zymography and protein expression by Western blot.

106 einases-1 (TIMP-1) by UDCA was studied using zymography and qRT-PCR.

107 serum and liver tissue MMP9 as evidenced by zymography and quantitative real-time PCR.

108 Zymography and real-time polymerase chain reaction analy

109 mal transition (EMT) were investigated using zymography and real-time qPCR.

110 ng the Dimethylmethylene Blue assay, gelatin zymography and reverse gelatin zymography respectively.

111 ected to assess MMP-2 and TIMP-2 activity by zymography and reverse zymography, proteins by Western b

112 inase 1 (TIMP-1) and TIMP-3/4 as assessed by zymography and reverse zymography.

113 hage marker CD68 expression were assessed by zymography and reverse-transcription polymerase chain re

114 mRNA expression were analyzed using gelatin zymography and RT-PCR, respectively.

115 ll three plant species were characterized by zymography and those of white birch were fully identifie

116 ignal, MMP activity as determined by in situ zymography and valvular inflammation by CD68 staining we

117 MMP secretion was measured by zymography and Western analysis, and expression in patie

118 In some mice, gelatin zymography and Western blot analyses were performed on p

119 membrane proteins were extracted for gelatin zymography and Western blot analyses.

120 Gelatin zymography and Western blot analysis revealed that the a

121 Gelatin zymography and Western blot data showed that only doxycy

122 Casein zymography and Western blot of m-calpain were performed

123 Incubation media were subjected to zymography and Western blot to detect activity and expre

124 Analysis by zymography and Western blotting showed that KSHV-infecte

125 -2 and -14 levels were determined by gelatin zymography and Western immunoblot analysis.

126 changes in MMPs and TIMPs were evaluated by zymography and Western immunoblot assay.

127 a, and MMP and TIMP levels were evaluated by zymography and Western immunoblot.

128 TIMP-2 using substrate gel electrophoresis (zymography) and Western blot analysis.

129 talloproteinase-2 (MMP2) activities (gelatin zymography), and cellular contents of MMP2, tissue inhib

130 significant knockdown of MMP-9 activity, per zymography, and a reversal of striatal rADC (p = 0.004,

131 x-metalloproteinases (MMPs) was evaluated by zymography, and apoptosis was evaluated by TUNEL and cas

132 uantitative reverse transcriptase (qRT)-PCR, zymography, and chromatin immunoprecipitation.

133 ubstrate assays, Western blotting, cathepsin zymography, and computational analyses, we uncovered cat

134 Immunoblot analysis, zymography, and ELISA were used to demonstrate the synth

135 activity in peritoneal fluid was assayed by zymography, and expression of tissue plasminogen activat

136 lymerase chain reaction, immunoblot, in situ zymography, and functional analyses.

137 MMP-9 in tear washings was evaluated by zymography, and gelatinase activity in the cornea and co

138 phies, and characterized by SDS-PAGE, casein zymography, and immunoblotting.

139 Using immunoblotting, in situ zymography, and immunofluorescence, we found that LTP in

140 reverse transcription-PCR (RT-PCR), gelatin zymography, and immunohistochemistry assays, the express

141 y, real-time RT-PCR, ELISA, activity assays, zymography, and immunohistochemistry.

142 MMP production was measured by gelatin zymography, and MMP-13 production and activation were de

143 evaluated by immunocytochemistry (ICC), WB, zymography, and RT-PCR.

144 2 and MMP-9 gelatinase activity assessed by zymography, and specific MMP 2/9 inhibitors significantl

145 rmed by immune fluorescence, flow cytometry, zymography, and stimulation of neutrophils.

146 assay, MMP activity was measured by gelatin zymography, and TIMP activity was determined by reverse

147 P-2 and -9 gelatinolytic activity by gelatin zymography, and tissue inhibitors of metalloproteinases

148 2 protein by Western blot, MMP-2 activity by zymography, and type IV collagen accumulation by ELISA.

149 uated via Multiplex MMP antibody arrays, gel zymography, and Western blot, which in turn proteolytica

150 These data were confirmed by immunostaining, zymography, and Western blotting.

151 introduces electrophoretic transfer protein zymography as one solution to this problem.

152 Additionally, the PLENZ zymography assay reports the kinetic properties of CIP i

153 olamine) photodegradation products, and in a zymography assay, reaction of collagen IV with products

154 activities of tPA and uPA were determined by zymography assays.

155 activator (uPA) activities were assessed by zymography assays.

156 e expression and activity by gel and in situ zymography at 24 hours, 48 hours, and 1 week after treat

157 Here we report a novel zymography-based technique, called the IHZ(TM) assay, fo

158 In situ zymography confirmed that SB-3CT suppressed gelatinase a

159 Gelatin zymography confirmed the expression of MMP-9 and MMP-2 i

160 d the presence of both MMP-2 and MMP-9 while zymography could only detect MMP-2.

161 Using zymography, CTGF increased the latent and active forms o

162 In situ zymography demonstrated a robust increase in MMP activit

163 Analysis of microsomes by zymography demonstrated a single band of proteolytic act

164 Gelatin zymography demonstrated activity of MMPs released into t

165 Zymography demonstrated enzyme activity in all synovium

166 Reverse fibrin zymography demonstrated free PAI-1 in cellular releasate

167 Conversely, collagen-based in situ zymography demonstrated markedly diminished collagenase

168 In situ zymography demonstrated that activated MMP-9 surrounded

169 Zymography demonstrated that unchallenged cells produced

170 Zymography demonstrated the release of four gelatinolyti

171 clin E processing assay, in combination with zymography, demonstrated that I3C, but not its natural d

172 quid extraction technique followed by casein Zymography detection.

173 Zymography detects and characterizes proteolytic enzymes

174 lastase B and alkaline protease, and gelatin zymography differentiated all four proteases.

175 s for MMP1 and MMP3, respectively) substrate zymography, digestion was increased with supernatants fr

176 Casein zymography distinguished protease IV from elastase B and

177 MMPs were assessed by zymography, DQ-gelatin degradation solution assays, and

178 Six in vitro assays were studied: zymography, elastin congo red assay, staphylolytic assay

179 loidin staining, MMP-2 activation by gelatin zymography, expression of MT1-MMP by quantitative real-t

180 ven gelatin proteolysis, measured by gelatin zymography, FITC-gelatin conversion, and DQ-gelatin degr

181 in) of serine proteases, assessed by in situ zymography, followed by serine-protease-mediated degrada

182 Using gel zymography for matrix metalloproteinase (MMP) detection

183 The usefulness of zymography for molecular weight determination and proteo

184 Purified recombinant PASP was analyzed by zymography for protease activity.

185 The developed AuNP assay was compared with zymography for qualitative detection of urinary HAase ac

186 espective inhibitors were analyzed by casein zymography, immune assays, and quantitative polymerase c

187 eated MV fraction was subjected to substrate zymography, immunoblotting, and substrate activity assay

188 o be decreased in DN, was investigated using zymography in a DN mouse model confirming the prediction

189 was activated by heregulin-beta1 as shown by zymography in both SKBr3 and MCF-7 breast cancer cell li

190 S-PAGE, and by in situ fluorogenic substrate zymography in RPE/choroid sections.

191 c gelatin and peptide substrates, by gelatin zymography in SDS-PAGE, and by in situ fluorogenic subst

192 MMP activity, detected by in situ zymography, increased in response to injury and was maxi

193 Gelatin zymography indicated increased secretion of active matri

194 Gelatin zymography indicated that matrix metalloproteinase 2 (MM

195 Casein zymography indicated the presence of two elastase-like a

196 Here, we used casein zymography, inhibitor profiling, affinity pull-down, and

197 In situ zymography investigations on skin sections from human ka

198 CD-1 mice, we show that MMP-9 expression by zymography is increased in the injured striatum compared

199 examined by immunohistochemistry and in situ zymography (ISZ), respectively.

200 Zymography measured the relative activity levels of MMP-

201 We have developed a novel in situ zymography method that uses a synthetic substrate conjug

202 On zymography, MMP-1 and -2 did not change.

203 d -9 expression was determined using gelatin zymography, MMP-1 by western blotting and ELISA and tiss

204 Zymography of angioreactors from MMP-deficient and contr

205 Western blot analysis and gelatin zymography of aortic extracts revealed that MMP-14 defic

206 Zymography of extracellular protease activity shed into

207 Gelatin zymography of extracts from the stria vascularis confirm

208 zymography of whole eye extracts and in situ zymography of retinal tumors showed strong gelatinase ex

209 In situ zymography of the caudal forebrain revealed that testost

210 microg/ml) to growth-arrested HMC for 72 h, zymography of the conditioned medium established that on

211 In situ zymography of the proximal jejunum reveals increased gel

212 Gel zymography of whole eye extracts and in situ zymography

213 Western blots), and gelatinolytic activity (zymography) of MMP-2 (2-fold), while not affecting TIMP-

214 ties of MMP-2/9 were corroborated by in situ zymography on frozen tissue sections.

215 -type-specific markers with MMP9 and gelatin zymography on the isolated cell populations, we identifi

216 By zymography, OPN and tumor necrosis factor (TNF)-alpha pr

217 extent of MMP activity was determined by gel zymography or enzyme-linked immunosorbent assays.

218 -9 content by substrate gel electrophoresis (zymography) or Western blotting.

219 crease in MMP-9 activity detected by gelatin zymography (P<0.01).

220 Gelatin zymography performed on TAA segments confirmed the absen

221 These activatable zymography probes (AZP) detected dysregulated protease a

222 We used substrate zymography, reverse zymography, proteinase inhibitors, and partial sequencin

223 nd TIMP-2 activity by zymography and reverse zymography, proteins by Western blot, and type IV collag

224 icrosensors were more sensitive than gelatin zymography; PSi microsensors detected the presence of bo

225 blot to determine proteoglycan degradation, zymography, radiolabeling to determine chondrocyte biosy

226 ected to immunohistochemistry, gelatinolytic zymography, real-time PCR, and Western blot analysis of

227 protein was proteolytically active in casein zymography, requiring divalent zinc.

228 ssay, gelatin zymography and reverse gelatin zymography respectively.

229 1 and MMP-9 was also determined by ELISA and zymography, respectively, in the absence or presence of

230 re determined by Matrigel assays and gelatin zymography, respectively.

231 were assayed using Western blot analysis and zymography, respectively.

232 d using (3)H-acetylated collagen and gelatin zymography, respectively.

233 y mRNA analyses, immunoblotting, and gelatin zymography, respectively.

234 sues were examined with Western blotting and zymography, respectively.

235 ntitative RT-PCR, ELISA, immunoblotting, and zymography, respectively.

236 Gelatin zymography results showed EGCG inhibits IL-6/soluble IL-

237 One- and two dimensional zymographies revealed a single polypeptide band with pro

238 served among T.b. and T.u. PABs, and reverse zymography revealed different bands, often not correlati

239 Gelatine zymography revealed different gelatinase activity patter

240 , flow cytometry for MHC class I and gelatin zymography revealed that microglial activation and expre

241 Gel zymography revealed that MMP-9 was activated and upregul

242 Casein zymography revealed that the effect of L-MIM involves a

243 At the site of degradation, in situ zymography revealed that the gelatinolytic activity, mai

244 Zymography revealed that the smaller caspase-7 product (

245 Zymography reveals that a protease activity, present in

246 real-time polymerase chain reaction, gelatin zymography, reverse transcriptase-polymerase chain react

247 Zymography, reverse zymography and ELISA (enzyme-linked

248 We used substrate zymography, reverse zymography, proteinase inhibitors, a

249 Furthermore, enzyme kinetic assays and zymography showed a higher LDH enzyme activity in LNCaP

250 Enzyme zymography showed attenuated matrix metalloproteinase-2

251 Gel zymography showed elevations in gelatinase (MMP-2 and MM

252 med by PCR) revealed sixfold more MMP-9, and zymography showed greater enzyme activity in the infecte

253 protein when compared with controls, and gel zymography showed increased MMP protein levels.

254 Gelatin zymography showed reduced levels of MMP-9 in the mt-ERK-

255 Gelatin zymography showed strong MMP activities at early time po

256 Fluorometric assay and zymography showed that adhesives with MMP inhibitors had

257 Protease activity visualized by gel zymography showed that after nerve crush, the upregulati

258 Zymography showed that EGCG blocked constitutive, IL-1be

259 In situ zymography showed that gelatinase activity was mostly co

260 In situ zymography showed that gelatinase activity was mostly co

261 Zymography showed that MMP-9 activity, which was very lo

262 hern blotting, Western blotting, and gelatin zymography showed that MMP-9 expression was significantl

263 RNase protection assays and plasminogen zymography showed that urokinase-type plasminogen activa

264 Zymography showed there were reduced amounts of active e

265 hemical and histological analyses, including zymography, simultaneous detection of perfused capillari

266 Zymography studies have also demonstrated increased acti

267 Our gelatin zymography studies on these two secreted gelatinases, pr

268 Moreover, elastin gel zymography studies showed that gel pretreatment with AlC

269 e IHZ assay represents a new type of in situ zymography technique that can be used for the screening

270 We describe a sensitive zymography technique that utilizes an automated microflu

271 Zymography techniques are routinely used to quantify pro

272 using enzyme-linked immunosorbent assays and zymography techniques that revealed MMP-1, -2, -3, and -

273 nt (BALB/c) mice before and after infection; zymography tested enzyme activity for MMP-2 and -9.

274 MMP9 activity in brains, measured by gelatin zymography, than mock-infected mice.

275 By zymography, the enzyme presented approximately 65 kDa, a

276 Samples were also prepared for gel zymography to detect the presence of MMP-2, and for Nort

277 We also demonstrate the use of gelatin zymography to determine the effects of differentiation a

278 was determined by Western blot analysis and zymography, using conditioned medium and HCM cells.

279 In situ zymography validated these results.

280 this report a novel protocol for SDS gelatin zymography was established, and an increase of cathepsin

281 Substrate zymography was performed to determine levels of secreted

282 e activity and biofilm maturation, autolysin zymography was performed, which revealed an altered prof

283 inase-2 expression and activation by gelatin zymography was unchanged between groups, while its endog

284 Zymography was used to assess the activity of MMP-1, -2,

285 Gelatin zymography was used to measure changes in the amount of

286 SDS-PAGE zymography was used to measure MMP2 and MMP9 activities.

287 Furthermore, cathepsin K zymography was used to show that murine osteoclasts secr

288 in situ hybridization and gelatin substrate zymography) was demonstrated in EMMPRIN-enhanced tumors.

289 Using gelatin zymography, we detected constitutive MMP-2 activity in b

290 ray, quantitative PCR, Western blotting, and zymography, we found that TNF-alpha drastically increase

291 Using gelatin zymography, we showed increased pro and active MMP-2 act

292 transcription-polymerase chain reaction and zymography were performed to analyze the mRNA expression

293 Both Western blot and zymography were used to monitor the effects of noladin e

294 Real time PCR, Western blot, and zymography were used to quantify MMP mRNA, protein, and

295 MMP expression in the retina was analyzed by zymography, Western blot, and immunohistochemistry.

296 duction or activity was determined by ELISA, zymography, Western blotting and immunofluorescent stain

297 on (by Northern blot) and MMP-2 activity (by zymography), which resulted in an increase in invasivene

298 the assay sensitivity was 82.5% vs. 65% for zymography, while the specificity for both assays was 96

299 Reverse zymography with human saliva and Tenebrio molitor alpha-

300 , enzymatic activity was detected by in situ zymography within the HLs of both tested adhesives, with