ライフサイエンスコーパス: CDPK

1 CDPK activated novel vacuolar chloride (VCL) and malate

2 CDPK enzymatic activity previously has been detected in

3 CDPK exhibits a Ca(2+)-induced electrophoretic mobility

4 CDPK from guard cells phosphorylates the K+ channel KAT1

5 CDPK-activated VCL currents were also observed in red be

6 CDPK-silenced plants showed a reduced and delayed hypers

7 CDPKs and at least one SnRK have been implicated in deco

8 CDPKs and SnRKs are found on all five Arabidopsis chromo

9 CDPKs are encoded by large multigene families, and to as

10 CDPKs are present in plants and a specific subgroup of p

11 CDPKs commonly have an N-terminal kinase domain (KD) and

12 CDPKs contain an autoinhibitory junction (J) region whos

13 CDPKs have diverse regulatory functions, including invol

14 CDPKs provide the first example of a member of the calmo

15 ysis of the Arabidopsis genome identified 34 CDPKs, eight CRKs, two PPCKs, two PEPRKs, and 38 SnRKs.

16 A CDPK phosphorylation site was mapped to Ser(45) near a c

17 expressed in tobacco cells can be used as a CDPK activity reporter for in vivo studies.

18 ther properties in vitro and appears to be a CDPK.

19 ited form correlate with the activation of a CDPK isoform after in vivo stimulation.

20 We propose that Ca2+ activation of a CDPK normally occurs through intramolecular binding of t

21 To understand how the CaM-LD regulates a CDPK, a recombinant CDPK (isoform CPK-1 from Arabidopsis

22 Single channel recordings showed a CDPK-activated 34 +/- 5 pS Cl- channel.

23 We are reporting for the first time a CDPK gene in mungbean which is inducible by mechanical s

24 g a Ca2+-independent, constitutively active, CDPK* mutant.

25 introns supports the phylogenetic analysis; CDPK genes with similar intron/exon structure are groupe

26 ulin-like domain, suggest that the ancestral CDPK gene could have originated from the fusion of prote

27 activity of MAPKs and CDPK-like kinases, and CDPK and NADPH oxidases were involved in As-induced MAPK

28 pecies and calcium and activity of MAPKs and CDPK-like kinases were induced with increasing Cr(VI) co

29 markedly increased the activity of MAPKs and CDPK-like kinases, and CDPK and NADPH oxidases were invo

30 itial kinetics of calmodulin stimulation and CDPK inhibition, providing an example in plants for a po

31 the subcellular location of one Arabidopsis CDPK, AtCPK2, in detail.

32 ation of AtCPK5, a member of the Arabidopsis CDPK family.

33 In this Update, we analyze the Arabidopsis CDPK gene family and review the expression, regulation,

34 roaches, the characterization of Arabidopsis CDPKs provides a valuable opportunity to understand the

35 e have dissected the function of an atypical CDPK from Plasmodium falciparum, PfCDPK7.

36 Because CDPKs are absent from the host, these kinases are consid

37 s: calcium-dependent protein kinase (CDPKs), CDPK-related kinases (CRKs), phosphoenolpyruvate carboxy

38 pport a monophyletic origin for plant CDPKs, CDPK-related kinases, and phosphoenolpyruvate carboxylas

39 on, the interconversion of the corresponding CDPK forms could be induced in vitro in both directions

40 s genome is predicted to encode 34 different CDPKs.

41 riety of functions are mediated by different CDPKs.

42 lant protein kinases, including two distinct CDPKs, fail to mimic this stress signaling.

43 rotein kinase with a calmodulin-like domain (CDPK) in guard cell protoplasts of Vicia faba.

44 ed for half-maximal activity (K0.5) for each CDPK with syntide-2 as substrate were 0.06, 0.4, and 1 m

45 The calcium-binding properties of each CDPK were distinct.

46 nsition from the nonelicited to the elicited CDPK form was caused by a phosphorylation event and was

47 mbinant CDPKalpha in vitro and by endogenous CDPK in vivo.

48 g cascade in planta via unregulated enhanced CDPK activity can lead to off-type effects likely due to

49 ly one site, and this site is different from CDPK phosphorylation sites.

50 His6-CDPK alpha (1-328), which contained none of the CLD, was

51 ivated only 5-fold, but the activity of His6-CDPK alpha (1-398), which retained nearly half of the CL

52 of the chimeric enzyme, its addition to His6-CDPK alpha (1-398) resulted in activity that was only 6%

53 cal inhibitors indicated that the identified CDPK is independent of or is located upstream from a sig

54 bout the subcellular locations of individual CDPKs or the mechanisms involved in targeting them to th

55 cting predominantly the phosphorylated 70-kD CDPK form, was greater than in nonelicited samples.

56 domain by a Ca(2+)-dependent protein kinase (CDPK isoform CPK1), inhibits both basal activity ( appro

57 Calcium-dependent protein kinase (CDPK) family members regulate diverse parasitic processe

58 ty of calmodulin-like domain protein kinase (CDPK) is regulated by the direct binding of Ca2+.

59 ht-induced calcium-dependent protein kinase (CDPK) isolated from the common ice plant, Mesembryanthem

60 An isoform of Ca2+-dependent protein kinase (CDPK) phosphorylated multiple sites of LlEF-1alpha1 in a

61 The native Ca(2+)-dependent protein kinase (CDPK) responsible for in vivo inhibitory phosphorylation

62 by a maize calcium-dependent protein kinase (CDPK) significantly increased sucrose cleavage activity

63 ane-bound, calcium-dependent protein kinase (CDPK) that showed a shift in electrophoretic mobility fr

64 responsive calcium-dependent protein kinase (CDPK) was isolated from the common ice plant (Mesembryan

65 APK) and 1 calcium-dependent protein kinase (CDPK) were upregulated with short-term Cr(VI) treatment.

66 za sativa) calcium-dependent protein kinase (CDPK), CPK18, was identified as an upstream kinase of MA

67 kinase and calcium-dependent protein kinase (CDPK), were also upregulated.

68 n kinases: calcium-dependent protein kinase (CDPKs), CDPK-related kinases (CRKs), phosphoenolpyruvate

69 Calcium-dependent protein kinases (CDPK) are a major group of calcium-stimulated kinases fo

70 Ca(2+)-dependent protein kinases (CDPK) have a calmodulin-like domain (CaM-LD) tethered to

71 found in calcium dependent protein kinases (CDPK).

72 ly inhibit select calcium-dependent kinases (CDPKs), we instead demonstrate that these pathways are c

73 constitutive Ca2+-dependent protein kinases (CDPKs) activate ABA responses, the MED141-143IGH and G18

74 fferent from Ca2+-dependent protein kinases (CDPKs) and other serine/threonine kinases in plants.

75 Calcium-dependent protein kinases (CDPKs) are a class of calcium-binding sensory proteins t

76 Calmodulin-like domain protein kinases (CDPKs) are a family of calcium- but not calmodulin-depen

77 that four calcium-dependent protein kinases (CDPKs) are Ca(2+)-sensor protein kinases critical for tr

78 Calcium-dependent protein kinases (CDPKs) are distinct from protein kinases of mammals, and

79 Calcium-dependent protein kinases (CDPKs) are essential enzymes in the biology of protozoan

80 Calcium-dependent protein kinases (CDPKs) are expanded in apicomplexan parasites, especiall

81 Calcium-dependent protein kinases (CDPKs) are key players in a plant's response to environm

82 Calcium-dependent protein kinases (CDPKs) are plant-specific proteins that sense changes in

83 Ca(2+)-dependent protein kinases (CDPKs) are regulated by a C-terminal calmodulin-like dom

84 Calcium-dependent protein kinases (CDPKs) are specific to plants and some protists.

85 Calcium-dependent protein kinases (CDPKs) are structurally unique Ser/Thr kinases found in

86 Calcium-dependent protein kinases (CDPKs) comprise a large family of serine/threonine kinas

87 Calcium-dependent protein kinases (CDPKs) comprise the major group of Ca2+-regulated kinase

88 those of the Ca2+-dependent protein kinases (CDPKs) from Plasmodium, Eimeria, and several plants, and

89 for calmodulin-like domain protein kinases (CDPKs) have been identified in plants and Alveolate prot

90 parasite calcium-dependent protein kinases (CDPKs) have been shown to reduce infection in several pa

91 Calcium-Dependent Protein Kinases (CDPKs) in higher plants contain a C-terminal calmodulin-

92 Calcium-dependent protein kinases (CDPKs) of Apicomplexan parasites are crucial for the sur

93 Calcium-dependent protein kinases (CDPKs) play important roles in the life cycle of Plasmod

94 Calcium-dependent protein kinases (CDPKs) play key regulatory roles in the life cycle of th

95 family of calcium-dependent protein kinases (CDPKs) that have no direct homologues in the human host.

96 ing novel calcium-dependent protein kinases (CDPKs) that provides new insights into the roles of CDPK

97 lant-like" Ca(2+)-dependent protein kinases (CDPKs) transduces cytosolic Ca(2+) flux into enzymatic a

98 s of calmodulin-like domain protein kinases (CDPKs) were detected.

99 to encode calcium-dependent protein kinases (CDPKs).

100 r through calcium-dependent protein kinases (CDPKs).

101 The observation of a peroxisome-located CDPK suggests a mechanism for calcium regulation of pero

102 Many CDPKs are membrane-associated, presumably because of lip

103 To test the impact of modulating CDPK activity in sorghum as a means to enhanced abiotic

104 , the precise biological function(s) of most CDPKs remains elusive.

105 he subcellular targeting potentials for nine CDPK isoforms from Arabidopsis, as determined by express

106 s to ascertain the functions of noncanonical CDPKs.

107 (45) (S45/A) completely blocked the observed CDPK inhibition of both basal and calmodulin-stimulated

108 utophosphorylation and catalytic activity of CDPK are Ca2+ dependent.

109 ily, we performed a phylogenetic analysis of CDPK genomic sequences.

110 ybrid system can accelerate the discovery of CDPK substrates.

111 D to the autoinhibitory (junction) domain of CDPK alpha in a manner analogous to the activation of ca

112 Silencing correlated with loss of CDPK mRNA, whereas mRNA accumulation of mitogen-activate

113 Mutants of CDPK alpha from which all or part of the CLD had been de

114 n, recombinant CLD and truncation mutants of CDPK alpha were expressed in bacteria and highly purifie

115 crystal structure of the J-CaM-LD region of CDPK from Arabidopsis thaliana (AtCPK1), determined to 2

116 s synthesized and found to be a substrate of CDPK isoforms alpha, beta, and gamma.

117 ata indicate that the response to calcium of CDPKs is clearly unique among the CaM family.

118 ntiality of CDPK1 and CDPK7, the majority of CDPKs had no discernible phenotype for growth in vitro o

119 that provides new insights into the roles of CDPKs during Toxoplasma gondii infection.

120 This work reveals the critical roles of CDPKs in modulating JA homeostasis and highlights the co

121 l with the consensus phosphorylation site of CDPKs, its coding sequence was cloned and stably transfo

122 show that this kinase and a number of other CDPKs of similar Mr showed complex changes in elicitor-t

123 PfCDPK7 is very different from that of other CDPKs; it has a pleckstrin homology domain adjacent to t

124 Little is known regarding physiological CDPK targets.

125 Plant CDPK genes on one branch share common intron positions w

126 amecium genes differ from those in the plant CDPK genes in about 20 of 31 residues in the junction re

127 Plant CDPKs divide into two major branches.

128 The introns shared between protist and plant CDPKs presumably originated before the divergence of pla

129 with previously reported sequences for plant CDPKs at the protein level.

130 rons support a monophyletic origin for plant CDPKs, CDPK-related kinases, and phosphoenolpyruvate car

131 regulation, and possible functions of plant CDPKs.

132 gether with previous studies on a plasmodium CDPK suggests a model whereby even at normally low cytos

133 lated a cDNA clone encoding a novel protein, CDPK substrate protein 1 (CSP1).

134 h share common intron positions with protist CDPK genes.

135 ally, the calmodulin-like domains of protist CDPKs have intron positions in common with animal and fu

136 w the CaM-LD regulates a CDPK, a recombinant CDPK (isoform CPK-1 from Arabidopsis, accession no. L147

137 We isolated two related CDPK cDNAs (NtCDPK2 and NtCDPK3) from Nicotiana tabacum.

138 plants and ciliates, suggesting that related CDPKs may have a function in calcium-regulated secretion

139 e introduced a constitutively expressed rice CDPK-7 (OsCDPK-7) gene construct.

140 ugh previous studies have shown that several CDPKs play a role in controlling invasion, egress, and c

141 dant roles in vivo, we characterized soybean CDPK isoforms alpha, beta, and gamma, which share 60-80%

142 Antibodies to soybean CDPK alpha cross-react with CDPK.

143 Unmodified soybean CDPK alpha and a chimeric enzyme in which the calmodulin

144 demonstrates key positive roles of specific CDPKs in initial MAMP signalling.

145 These results suggest that CDPK may be an important component of Ca2+ signaling in

146 It has long been assumed that CDPKs are activated, like other Ca2+-regulated kinases,

147 tron placements supports the hypothesis that CDPKs, CRKs, PPCKs and PEPRKs have a common evolutionary

148 anscriptome profile comparison suggests that CDPKs are the convergence point of signalling triggered

149 The CDPK-SnRK superfamily consists of seven types of serine-

150 In these assays, the CDPK activity in elicited samples, reflecting predominan

151 These results show that members of the CDPK family differ in biochemical properties and support

152 To study the molecular evolution of the CDPK gene family, we performed a phylogenetic analysis o

153 vealed the presence of several copies of the CDPK gene.

154 ial role in stabilizing the structure of the CDPK regulatory apparatus.

155 transition is due to phosphorylation of the CDPK.

156 hen elicitor is subsequently added while the CDPK cannot be activated by elicitor upon forskolin trea

157 The CDPKs were inhibited by the general protein kinase inhib

158 tor there is a transient inactivation of the CDPKs before activation.

159 These CDPK transcripts are elevated after race-specific defenc

160 RNA blot analysis showed that the three CDPKs were expressed in most plant tissues examined and

161 ow Ca(2+) signaling activates egress through CDPKs, we performed a forward genetic screen to isolate

162 on event and was verified when antibodies to CDPK were used for protein gel blot analysis.

163 nition by CaM other features are specific to CDPKs, in particular the combination of the strong inter

164 revious studies on Plasmodium and Toxoplasma CDPKs suggest a role for the JD and CamLD in the regulat

165 Consistent with this model, a truncated CDPK (DeltaNC) in which the CaM-LD has been deleted can

166 We show here that a truncated CDPK lacking a CaM-LD (e.g. mutant delta NC-26H) can be

167 nic Nicotiana attenuata plants, in which two CDPKs, NaCDPK4 and NaCDPK5, were simultaneously silenced

168 Unexpectedly, CDPKs and MAPK cascades act differentially in four MAMP-

169 Unlike CDPK, CCaMK phosphorylated only one site, and this site

170 atter activity approached that of unmodified CDPK alpha and was half maximal at a CLD concentration o

171 se of catalytically impaired and unregulated CDPKs with the yeast two-hybrid system can accelerate th

172 sition was not inhibited by W-7, the in vivo CDPK activation probably is not the result of autophosph

173 To determine whether CDPK or other protein kinases could have a role in guard

174 owed weaker activation (22% as compared with CDPK).

175 odies to soybean CDPK alpha cross-react with CDPK.