ライフサイエンスコーパス: H. hepaticus

1 H. hepaticus causes chronic active hepatitis, with progr

2 H. hepaticus had a strong affinity for molecular H(2) (a

3 H. hepaticus infection elicited both T cell-mediated and

4 H. hepaticus present in feces and cecal samples from H.

5 H. hepaticus was cultured from fetal viscera of 2 of 11

6 H. hepaticus was orally inoculated into 30 axenic, outbr

7 H. hepaticus, which forms a spreading film on selective

8 H. hepaticus, which persists in the lower bowels and liv

9 H. hepaticus-induced colitis is associated with elevated

10 H. hepaticus-infected BALB-RagMin mice developed moderat

11 H. hepaticus-infected mice seroconverted by 2 weeks and

12 H. hepaticus-infected parental strains including A/J and

13 H. hepaticus-infected Rag2-/-, but not sham-dosed Rag2-/

14 H. hepaticus-infected wild-type mice did not develop inf

15 ith H. hepaticus in tissue sections and to a H. hepaticus protein (70 kd) in Western blots.

16 with regulatory cells at 4 or 12 weeks after H. hepaticus infection had reduced severity of inflammat

17 d no serum immunoglobulin G response against H. hepaticus.

18 obulin G1 (IgG1) and IgG2c responses against H. hepaticus, while animals challenged with the CDT-defi

19 s and failed to mount IgG1 responses against H. hepaticus.

20 2 p40 from p50(-/-)p65(+/-) mice ameliorates H. hepaticus-induced disease.

21 Diversity among H. hepaticus isolates was evaluated by means of a restri

22 The urease genes among H. hepaticus strains were also well conserved, showing 9

23 An H. hepaticus transposon mutant with a disrupted cdtABC c

24 ma production by IL-10 KO CD4(+) cells in an H. hepaticus antigen-specific manner.

25 mice, were divided equally into control and H. hepaticus-infected groups and euthanized at 18 months

26 The results of H. hepaticus culture and H. hepaticus-specific PCR concurred (i.e., both positive

27 itric oxide synthase (iNOS) mRNA levels, and H. hepaticus-specific IFN-gamma secretion by mesenteric

28 9% of H. hepaticus-infected F1 male mice and H. hepaticus was isolated from hepatic tissues of all F1

29 ry properties of the CdtB of H. pullorum and H. hepaticus were assessed on human intestinal and hepat

30 oocytes has shown that UreI of H. pylori and H. hepaticus can transport urea only at acidic pH, where

31 tabolic PutA flavoenzymes from H. pylori and H. hepaticus revealed that Helicobacter PutA generates r

32 Infected mice also developed sustained anti-H. hepaticus serum immunoglobulin G antibody responses a

33 ng invasive adenocarcinoma, possibly because H. hepaticus, in delaying the development of colitis, al

34 Here we investigate the interactions between H. hepaticus and host immune cells that may promote mutu

35 Colonization of the mucosa by H. hepaticus was associated with a decrease in the overa

36 In contrast, H. hepaticus infection of mdr1a-/- mice did not accelera

37 A cdtB-deficient H. hepaticus isogenic mutant (HhcdtBm7) was generated an

38 assay was developed to quantitatively detect H. hepaticus in mouse ceca and feces using the ABI Prism

39 r-stained liver sections is used to diagnose H. hepaticus infection.

40 e that harbored or did not harbor endogenous H. hepaticus.

41 flammation in IL-10 KO mice with established H. hepaticus-induced colitis.

42 efect in the regulation of IL-12 expression, H. hepaticus induced markedly higher levels of IL-12 p40

43 red for the development of colitis following H. hepaticus infection.

44 2 p40 show no intestinal pathology following H. hepaticus infection.

45 In situ hybridization for H. hepaticus 16S rRNA showed that the bacteria was found

46 ths after inoculation by culture and PCR for H. hepaticus colonization of the liver and cecum, and mi

47 (p.i.) were surveyed by culture and PCR for H. hepaticus in liver and intestinal tissues.

48 Sera from mice positive for H. hepaticus by PCR or histologic examination (n = 88),

49 d the need to include diagnostic testing for H. hepaticus in a murine health monitoring program.

50 mesenteric lymph node (MLN) cells alone from H. hepaticus-infected mutant mice.

51 d that the cotransfer of CD4(+) T cells from H. hepaticus-infected but not uninfected WT mice prevent

52 ing this cytotoxic activity were cloned from H. hepaticus.

53 carrying the cloned cdtABC gene cluster from H. hepaticus reproduced the cytotoxic activities seen wi

54 rRNA gene and is biochemically distinct from H. hepaticus.

55 portion of the urease structural genes from H. hepaticus genomic DNA.

56 icus present in feces and cecal samples from H. hepaticus-infected mice was readily quantified.

57 uch lower sensitivity (10(6)-fold) than from H. hepaticus DNA.

58 ompared to 14 of 44 of the mice (32%) having H. hepaticus cultured from their frozen liver tumors.

59 However, H. hepaticus was not considered a complicating factor, b

60 However, H. hepaticus-infected BALB-RagMin mice had a significant

61 These lines of evidence indicate that (i) H. hepaticus CDT plays a crucial role in the persistent

62 The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice l

63 In this study, we determined if H. hepaticus infection could prevent H. bilis-induced co

64 Hydrogenase activities in H. hepaticus were constitutive and not dependent on the

65 reviously described granulating cytotoxin in H. hepaticus.

66 st report profiling cecal gene expression in H. hepaticus-infected A/JCr mice.

67 ost infection by disrupting the putA gene in H. hepaticus.

68 tobacilli reduced intestinal inflammation in H. hepaticus-challenged IL-10-deficient mice despite sim

69 t of colonic, but not cecal, inflammation in H. hepaticus-infected anti-IL-10R-treated mice, demonstr

70 cant reduction of intestinal inflammation in H. hepaticus-infected IL-10(-/-) mice, suggesting an imp

71 suggesting a role for proline metabolism in H. hepaticus pathogenicity in vivo.

72 Two days after infection, H. hepaticus comprised a minor component of the mucosa-a

73 n forms of androgen interruption can inhibit H. hepaticus-induced hepatitis in young male mice, where

74 Rag-deficient hosts significantly inhibited H. hepaticus-induced inflammation and development of can

75 munoassays, and the quantities of intestinal H. hepaticus were evaluated by real-time PCR.

76 7BL/6 interleukin 10(-/-) mice with isogenic H. hepaticus mutants revealed that CDT expression is not

77 hepaticus in the ileum of female mice; (iv) H. hepaticus colonization was associated with down-regul

78 mice without characteristic hepatic lesions, H. hepaticus-specific DNA was amplified from the livers

79 Like H. hepaticus, it is a spiral bacterium with bipolar shea

80 d raises the possibility that H. canis, like H. hepaticus and H. bilis in mice, can cause inflammatio

81 ical bioassays conducted with B6C3F(1) mice, H. hepaticus has been regarded as a confounding factor b

82 By electron microscopy, H. hepaticus was noted infrequently and only in bile can

83 ic helicobacters, H. pylori and H. mustelae, H. hepaticus possesses a high level of urease activity.

84 The wild-type and putA mutant H. hepaticus strains displayed similar levels of infecti

85 However, a CDT-negative H. hepaticus mutant had a significantly diminished capac

86 Nongastric H. hepaticus produces urease similar to that of H. pylor

87 lastic liver lesions were observed in 69% of H. hepaticus-infected F1 male mice and H. hepaticus was

88 We have shown that although the ability of H. hepaticus to induce colitis in Rag-2(-/-) mice is inh

89 a critical role in inhibiting the ability of H. hepaticus to induce IL-12 p40.

90 Analysis of the gene expression in ceca of H. hepaticus infected mice revealed 25 up-regulated and

91 ucial role in the persistent colonization of H. hepaticus in SW mice; (ii) SW female mice are more re

92 d jejunum but only transient colonization of H. hepaticus in the ileum of female mice; (iv) H. hepati

93 The combination of H. hepaticus infection and CD45RB(high) CD4+ T-cell reco

94 ack of Th1-like ex-Th17 cells, the degree of H. hepaticus-triggered intestinal inflammation in mice i

95 this assay, the sensitivity for detection of H. hepaticus chromosomal DNA prepared from pure culture

96 vity is a potential virulence determinant of H. hepaticus that may play a role in the pathogenesis of

97 nscription factors during the development of H. hepaticus-associated liver tumors and may have releva

98 ess resulting from the relative dominance of H. hepaticus as a member of the community.

99 tope on the flagellar hook protein (FlgE) of H. hepaticus presented by I-Ab.

100 n study, suggesting that the urease genes of H. hepaticus are stable.

101 was conducted to determine the incidence of H. hepaticus infection and to evaluate different diagnos

102 ales, suggesting transplacental infection of H. hepaticus.

103 ction exist, and to analyze the influence of H. hepaticus on hepatocyte proliferation, a longitudinal

104 These results suggest that inhibition of H. hepaticus-induced IL-12 p40 expression by NF-kappaB s

105 Genomic DNAs from 11 isolates of H. hepaticus from the United States, Germany, France, an

106 The level of H. hepaticus serum antibody was highest in experimentall

107 igate the role of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generat

108 nomodulatory role that allows persistence of H. hepaticus and that in IL-10-/- mice this alteration o

109 and was able to detect as little as 5 pg of H. hepaticus, H. bilis, or H. muridarum DNA.

110 n mice with a membrane digest preparation of H. hepaticus as the antigen.

111 tic approaches for assessing the presence of H. hepaticus in livers lacking characteristic lesions.

112 study confirms the widespread prevalence of H. hepaticus in mice, its potential to confound experime

113 deficient mice despite similar quantities of H. hepaticus in cocolonized animals.

114 The results of H. hepaticus culture and H. hepaticus-specific PCR concu

115 nosorbent assay (ELISA) for serodiagnosis of H. hepaticus infection in mice with a membrane digest pr

116 sitive and specific for the serodiagnosis of H. hepaticus infection in mice.

117 By PFGE, the genomic size of H. hepaticus is estimated to be roughly 1.3 Mb, which co

118 cytotoxic activities seen with sonicates of H. hepaticus.

119 oted in sequential isolates of one strain of H. hepaticus during an 18 month in vivo colonization stu

120 The putA mutant strain of H. hepaticus exhibited increased proline levels and resi

121 ated A/JCr mice with one of three strains of H. hepaticus: type strain Hh3B1, which contains the comp

122 ful in subsequent epidemiological studies of H. hepaticus when the source and method of spread of thi

123 ocyte proliferation, a longitudinal study of H. hepaticus-infected A/JCr mice was undertaken.

124 In contrast, anti-IL-17F had no effect on H. hepaticus-induced intestinal pathology.

125 epatocellular carcinoma was diagnosed in one H. hepaticus-infected mouse.

126 ne whether infection with either H. bilis or H. hepaticus would accelerate the development of inflamm

127 The deduced amino acid sequence of a partial H. hepaticus ureB gene product exhibited 75% identity an

128 e deduced amino acid sequence of the partial H. hepaticus ureA gene product was found to exhibit 60%

129 in the presence of a single murine pathogen, H. hepaticus.

130 ented colitis development despite persistent H. hepaticus infection in recipient mice.

131 s harbouring B. fragilis not expressing PSA, H. hepaticus colonization leads to disease and pro-infla

132 e to whole Helicobacter bacteria (H. pylori, H. hepaticus, and H. felis) was mediated not by TLR4 but

133 high sensitivity and specificity to quantify H. hepaticus in experimentally infected mouse models as

134 o obtain enzymatic activity from recombinant H. hepaticus urease; special conditions including NiCl2

135 ver tumors, argyrophilic bacteria resembling H. hepaticus were observed in liver sections, associated

136 In the absence of an intact IL-10 signaling, H. hepaticus induces an IL-23-driven inflammatory respon

137 d mesenteric lymph node cells with a soluble H. hepaticus antigen (Ag) preparation.

138 thogenic enterohepatic Helicobacter species, H. hepaticus.

139 Strong H. hepaticus-reactive antibody responses as measured by

140 ected animals throughout the 18-month study, H. hepaticus was consistently isolated from the lower bo

141 plenocyte populations are unable to suppress H. hepaticus-induced colitis in p50(-/-)p65(+/-)Rag-2(-/

142 phlonius was found to be less prevalent than H. hepaticus and H. rodentium but as prevalent as H. bil

143 Together, our data support the concept that H. hepaticus infection results in the induction in WT mi

144 We conclude that H. hepaticus does not induce or potentiate disease in ou

145 In this study we have demonstrated that H. hepaticus challenge of macrophages induces ERK activa

146 These findings indicate that H. hepaticus has urease structural genes which are homol

147 ys completed in our laboratory indicate that H. hepaticus is widespread in academic and commercial mo

148 d restriction enzyme analyses indicated that H. hepaticus and H. bilis infections are widespread in l

149 We here show that H. hepaticus Ag (SHelAg)-specific CD4+ Th1 clones transf

150 Our results show that H. hepaticus triggers early IL-10 induction in intestina

151 These results suggest that H. hepaticus induces ERK activation by a pathway depende

152 The data suggest that H. hepaticus, which is present in many research colonies

153 The H. hepaticus enzyme coupled H(2) oxidation to reduction

154 The H. hepaticus urease cluster contains a homolog of each g

155 The H. hepaticus urease gene cluster was expressed in Escher

156 The H. hepaticus-infected mouse will provide an ideal model

157 The aim of this study was to examine the H. hepaticus genome by pulsed-field gel electrophoresis

158 probe for this assay were generated from the H. hepaticus cdtB gene (encoding subunit B of the H. hep

159 However, the H. hepaticus urease structural gene sequences have not b

160 paticus cdtB gene (encoding subunit B of the H. hepaticus cytolethal distending toxin).

161 equivalent to approximately 14 copies of the H. hepaticus genome based on an estimated genome size of

162 timated by real-time PCR quantitation of the H. hepaticus-specific cytolethal distending toxin gene a

163 In this H. hepaticus-challenged IL-10-deficient murine colitis m

164 the presence of immunoglobulin G antibody to H. hepaticus and changes in the liver enzyme alanine ami

165 ve responses by splenic mononuclear cells to H. hepaticus antigens.

166 specific CD4+ Th1 clones transfer disease to H. hepaticus-infected T cell-deficient RAG KO hosts.

167 tis and the immune response of A/JCr mice to H. hepaticus infection.

168 Furthermore, prior exposure of donor mice to H. hepaticus significantly enhances antitumor potency of

169 onius is genetically most closely related to H. hepaticus.

170 e; (ii) SW female mice are more resistant to H. hepaticus colonization than male mice; (iii) there wa

171 eptible and C57BL/6NCr mice are resistant to H. hepaticus-induced hepatitis.

172 ich develop a significant immune response to H. hepaticus associated with prominent multifocal mononu

173 on of a cell-mediated Th1 immune response to H. hepaticus infection in the A/JCr mouse should prove v

174 s equipped to mount a successful response to H. hepaticus infection, increasing colon cancer risk.

175 patic gene expression profile in response to H. hepaticus infection.

176 To identify gene expression specific to H. hepaticus-induced hepatitis and progression to hepato

177 A/JCr mice are particularly susceptible to H. hepaticus-induced hepatitis and subsequent developmen

178 Only animals infected with wild-type type H. hepaticus developed significant typhlocolitis.

179 were experimentally infected with wild-type H. hepaticus and a CDT-deficient isogenic mutant.

180 Both wild-type H. hepaticus and the CDT-deficient mutant successfully c

181 Animals infected with wild-type H. hepaticus developed serum immunoglobulin G1 (IgG1) an

182 Animals infected with wild-type H. hepaticus exhibited severe typhlocolitis at 8 months

183 tectable in IL-10-/- mice, whereas wild-type H. hepaticus persisted for the 8-month duration of the e

184 rechallenge with either mutant or wild-type H. hepaticus.

185 To analyze whether H. hepaticus persists in specified ecological niches, to

186 lis accelerates development of colitis while H. hepaticus delays disease.

187 n wild-type macrophages after challenge with H. hepaticus.

188 obacillus paracasei and then challenged with H. hepaticus.

189 Coinfection with H. hepaticus also suppressed H. pylori-induced elevation

190 ions (59%) were most commonly colonized with H. hepaticus alone or in combination with other Helicoba

191 t have histological features compatible with H. hepaticus infection.

192 erleukin-4 (IL-4) or IL-5 when cultured with H. hepaticus outer membrane proteins.

193 anling A/JCr mice (n = 67) were gavaged with H. hepaticus or vehicle.

194 Mice were gavaged with H. hepaticus; DFO feeding was continued; and mice were s

195 However, mice infected with H. hepaticus and H. pylori (HhHp mice) developed more se

196 contact mice were persistently infected with H. hepaticus as identified by culture and PCR, in both t

197 mice; all were confirmed to be infected with H. hepaticus by culture and PCR.

198 Mice infected with H. hepaticus developed progressively severe perivascular

199 Male A/JCr mice were infected with H. hepaticus or vehicle at 4 weeks and randomized into s

200 rt that hepatitis of male mice infected with H. hepaticus show significant increases in the oxidative

201 ntact mice became persistently infected with H. hepaticus, lesions were less severe and the levels of

202 s and carcinogenesis in livers infected with H. hepaticus.

203 Concurrent infection with H. hepaticus may confound studies that have been attribu

204 Infections with H. hepaticus and H. bilis have been associated with hepa

205 cking the p50/p105 subunit of NF-kappaB with H. hepaticus.

206 with natural infection of SCID/NCr mice with H. hepaticus and that lesions are progressive with age.

207 ins, derived from A/J and C57BL/6 mice, with H. hepaticus to determine the genetic basis of resistanc

208 genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enema

209 und that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histolo

210 had mice that were either monoinfected with H. hepaticus, monoinfected with seven other Helicobacter

211 10 C57BL/6 mice were inoculated per os with H. hepaticus, Helicobacter muridarum, or H. bilis.

212 tibody to heat shock protein 70 reacted with H. hepaticus in tissue sections and to a H. hepaticus pr

213 they responded when stimulated in vitro with H. hepaticus and Helicobacter typhlonius Ag, but not whe

214 retion, or T-cell activation with or without H. hepaticus.

215 ) in females (P < 0.016 and 0.031 between WT H. hepaticus-infected and sham-dosed females, respective

216 in both female and male mice colonized by WT H. hepaticus or in males transiently colonized through 8

217 iii) there was persistent colonization of WT H. hepaticus in cecum, colon, and jejunum but only trans

218 d 16 wpi; however, colonization levels of WT H. hepaticus in the cecum and colon of male mice were ap

219 vely) responses in the mice infected with WT H. hepaticus when compared to HhcdtBm7 at 16 wpi.

220 Infection with WT H. hepaticus, but not HhcdtBm7, at 8 wpi was associated

221 Wild-type (WT) H. hepaticus was detected in the corresponding intestina