ライフサイエンスコーパス: Pronase

1 sates ranged from 0.1% (protex 26L) to 3.7% (pronase).

2 tunicamycin, endoglycosidase H, or protease (pronase).

3 to hydrolysis with trypsin only and trypsin-pronase.

4 ein precipitation followed by digestion with Pronase.

5 modes after digestion with bead-immobilized Pronase.

6 ore and after treatment of intact cells with pronase.

7 calized in the small complex protection from pronase.

8 moved by internal perfusion of the axon with pronase.

9 d after treatment with chondroitinase AC and pronase.

10 Cells prior treated with pronase (0.5 U/ml) displayed a complete inhibition of in

11 The nonspecific protease mixture pronase also prevented its formation and resulted in the

12 It is sensitive to pronase and periodate, indicating that it is likely a gl

13 Ovomucin hydrolyzed by pronase and protex 26L showed molecular weight (Mw) dist

14 urface bound, as shown by its sensitivity to pronase and the staining pattern revealed by in situ amp

15 od, spleen, or lymph nodes were treated with pronase and then used in a three-color FCXM.

16 The transferable factor(s) was resistant to pronase and trypsin digestion, was heat stable at 56 or

17 Pronase and Viscozyme improved the extraction of insolub

18 eatment with protein-modifying agents (heat, pronase, and N-bromosuccinimide) and was blocked by prot

19 s in the (rat) brain and TG were digested by pronase, and THs were extracted with a solid-phase extra

20 leased from their complexes by the action of Pronase, but not DNAse, RNAse, or strong salt solutions,

21 The use of unspecific proteases such as Pronase can largely overcome this problem by generating

22 Pronase challenge experiments suggested that the reduced

23 activated HSCs in mice, based on retrograde pronase-collagenase perfusion of the liver and subsequen

24 eatly narrowed by proteolytic digestion with Pronase, confirming that the initial spin-trapped radica

25 Proteolysis by Pronase converted the anisotropic electron paramagnetic

26 A glycopeptide moiety was isolated from a pronase digest of Fap1 and purified by immunoaffinity ch

27 n site was determined by Edman sequencing of pronase-digested Ambn, which gave HPPPLPXQPS, indicating

28 patic cells were isolated by collagenase and pronase digestion followed by centrifugal elutriation, a

29 Small glycopeptides generated by Pronase digestion of gp69/64 also inhibited sperm-egg bi

30 Pronase digestion of the G248E P450(cam) mutant after co

31 Earlier studies on the effect of Pronase digestion on receptor activity suggest that this

32 Incorporation of a pronase digestion step prior to the immunoaffinity LC-MS

33 ptide fragments were susceptible to complete Pronase digestion to their constituent amino acids.

34 Glycopeptides (by Pronase digestion) were separated by anion-exchange chro

35 isopeptide was identified in the exhaustive Pronase digests of native EF-P and recombinant EF-P isol

36 ations were fragmented using incubation with Pronase E and/or formic acid, and in each case a complet

37 roteins, as pretreatment of pneumococci with pronase E but not sodium periodate significantly reduced

38 nces and yield fragmentation profiles across Pronase E concentrations which can readily be used by ot

39 Extensive Pronase E digestion of unlabeled Muclin was used to prod

40 At Pronase E loadings of 10 mg, the analysis indicated that

41 ate the influence on digestion efficiency of Pronase E loadings, salinity, natural organic matter con

42 The cells are then treated with Pronase E to degrade residual surface-bound material, an

43 Enzymatic digestion of polypeptides using Pronase E, a protease cocktail, proved preferable to com

44 Overall, Pronase E-generated peptide standards were a rapid and e

45 As a pilot study, Pronase E-generated standards from an Abl kinase sensor

46 ed by 57% when bacteria were pretreated with pronase E.

47 ectins did not affect Ib interactions, while pronase effectively prevented Ib binding to the cell sur

48 fast inactivation by the proteolytic enzyme pronase eliminated charge immobilization, while the spec

49 antibody strength assignment, and the use of pronase for crossmatch.

50 oligosialic chains discovered earlier in the Pronase-generated glycopeptide fraction isolated from th

51 Finally, hydrolysates obtained using trypsin-pronase had a greater antioxidant capacity (ORAC) than c

52 e incubated in vitro with LPS, flagellin, or pronase-inactivated flagellin in the presence or absence

53 Selective digestion with pronase, NaOH/NaBH(4), heparinase I, or low pH nitrous a

54 ctivity was heat labile and was abolished by pronase or acid-glycine (pH 2.2) treatment but not by li

55 (0.1%, 1 min each) but not by chymotrypsin, Pronase, or papain (0.1%, up to 2 min each).

56 alpha-p-tosyl-L-lysine chloromethyl ketone), pronase, or proteinase K, suggesting that the functional

57 Murine HSCs were isolated by collagenase-pronase-perfusion, and density gradient centrifugation.

58 trypsin or neuraminidase, had no effect, but pronase pretreatment of RBC resulted in a slight increas

59 orseradish peroxidase (HRP) were digested by Pronase, protected by FmocCl, and efficiently separated

60 pecific and broad specific proteases such as Pronase, proteinase K, pepsin, papain, and subtilisin.

61 eatment of mucin with periodate but not with pronase reduced adherence.

62 The proteolytic enzyme Pronase reduced the uptake of transferrin, suggesting th

63 Pronase-resistant fluorescence was detectable in vesicle

64 each HS-glycosaminoglycan region comprised a pronase-resistant peptide separating two HS chains.

65 2H5 and KM93 bound to pronase-resistant structures, indicating that the mAbs d

66 rotease N), and 2.4% (flavourzyme) to 46.3% (pronase), respectively.

67 Enzymatic hydrolysis of FPI with trypsin and pronase resulted in a hydrolysate that was fractionated

68 lated WT SOD1 with trypsin, chymotrypsin, or Pronase revealed that the first 63 residues of the N ter

69 However, it is pronase sensitive and dependent on N-linked glycosylatio

70 We observed that P- and E-selectin bound to pronase-sensitive ligands on murine monocytic WEHI-3 cel

71 Prior treatment of B cells with pronase to remove cell-surface Ig or treatment with BCR-

72 ell FCXM, we utilized the proteolytic enzyme pronase to remove Fc receptors from lymphocytes before t

73 xplored a method using a nonspecific enzyme, pronase, to generate small glycopeptides (between two an

74 e-specific N- and O-glycopeptide analysis of Pronase treated glycoproteins with integrated, sequentia

75 A total of 167 T- and B cell FCXMs using pronase-treated and untreated cells were performed.

76 Utilization of pronase-treated lymphocytes improves both the sensitivit

77 h a very similar EPR spectrum to that of the Pronase-treated MNP/(center dot)SOD, suggesting that the

78 structures resembling straight filaments or Pronase-treated paired helical filaments raises fundamen

79 ptide cleavage by pepsin or by up to 20 h of Pronase treatment altered fiber assembly kinetics, but t

80 In this study, pronase treatment improved the specificity of B cell FCX

81 Pronase treatment inhibited transfection, whereas dilute

82 Pronase treatment of RBCs only modestly altered choleste

83 In contrast, prolonged (>20 h) Pronase treatment resulted in cleavage of the triple hel

84 Pronase treatment resulted in improved sensitivity of th

85 a-loaded and dried devices were subjected to pronase treatment yielding the alkylated dipeptide hydro

86 rimary granules was significantly reduced by pronase treatment, boiling, high ionic strength, and mag

87 After pronase treatment, T and B cell FCXMs of each patient be

88 After pronase treatment, the actual channel values of the nega

89 However, after pronase treatment, the inhibitory activity of both bikun

90 chin, and prodelphinidin dimer A compared to Pronase treatment.

91 d from the cell surface by either trypsin or pronase treatment.

92 fibers were not affected by either pepsin or Pronase treatment.

93 , were extracted with Viscozyme but not with Pronase treatment.

94 Pretreatment of human EGP with pronase, trypsin, 2-ME, or heating did not interfere wit

95 the surface of human erythrocytes included: pronase, trypsin, beta-mercaptoethanol (2-ME), and heati

96 that BBMs do substantially protect CPE from pronase when this toxin is localized in large complex.

97 Glycoproteins were digested with Pronase yielding primarily glycopeptides and amino acids