ライフサイエンスコーパス: alpha-gustducin

1 ce deficient in the gustducin alpha-subunit (alpha-gustducin).

2 hereas sucralose internalized T1R2, T1R3 and alpha-gustducin.

3 t receptor antagonist lactisole or siRNA for alpha-gustducin.

4 to lingual taste cells and strongly express alpha-gustducin.

5 ce, but not in knockout mice lacking T1R3 or alpha-gustducin.

6 Mouse intestinal L cells also express alpha-gustducin.

7 y pertussis toxin and by genetic deletion of alpha-gustducin.

8 t not all, bitter-responsive cells expressed alpha-gustducin.

9 5 kDa (SNAP-25), and to a lesser extent with alpha-gustducin.

10 li was reduced by 70% in mutant mice lacking alpha-gustducin.

11 ets using the molecular markers Vimentin and alpha-Gustducin.

12 group antigen, whether or not they expressed alpha-gustducin.

13 ceptors and the tastant-associated G protein alpha-gustducin.

14 for immunoreactivity to an antibody against alpha-gustducin.

15 lls express T2R "bitter-taste" receptors and alpha-gustducin, a G protein involved in chemosensory tr

16 fy the subset of type II cells that contains alpha-gustducin, a G-protein involved in bitter transduc

17 distributions of these antigens with that of alpha-gustducin, a G-protein subunit implicated in respo

18 A key molecule, alpha-gustducin, a primarily taste-specific G protein al

19 Alpha-gustducin, a taste cell-expressed G-protein alpha

20 We set out to determine whether alpha-gustducin also mediates umami taste and whether ro

21 Umami detection involving alpha-gustducin and alpha(t-rod) occurs in anteriorly pl

22 Both alpha-gustducin and alpha-transducin activate phosphodie

23 ys, we generated a dominant-negative form of alpha-gustducin and expressed it as a transgene from the

24 differentiated light cells that also express alpha-gustducin and may be involved in intercellular int

25 which were incubated with antibodies against alpha-gustducin and the human blood group A antigen.

26 Thus, proposed models for alpha-gustducin and those found by other laboratories ma

27 te receptors are coupled through G-proteins, alpha-gustducin and transducin, to activate phospholipas

28 ordings of single and double KO mice lacking alpha-gustducin and/or alpha(t-rod) confirmed the involv

29 e Cbeta2 (PLCbeta2) or the G-protein subunit alpha-gustducin, and serotonin (5HT) as markers of type

30 Both the gustatory G-protein, alpha-gustducin, and the cell-surface carbohydrate, the

31 chemical and genetic studies have implicated alpha-gustducin as a key component in the transduction o

32 control experiments, expression of wild-type alpha-gustducin as a transgene in alpha-gustducin-null m

33 All Lewis(b)-positive cells expressed alpha-gustducin, but only a fraction of alpha-gustducin-

34 Taste cells with alpha-gustducin could express either presynaptic protein

35 2P, introduced into the C-terminal region of alpha-gustducin critical for receptor interaction render

36 he normal vallate papilla had a mean of 8.37 alpha-gustducin-expressing cells and 5.22 A-expressing c

37 Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated by taste

38 (8.4)) from the upstream region of the mouse alpha-gustducin gene acts as a fully functional promoter

39 ly proving that the targeted deletion of the alpha-gustducin gene caused the taste deficits of the nu

40 lthough "knock-out" animals deficient in the alpha-gustducin gene clearly demonstrate that gustducin

41 essed with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-be

42 Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells p

43 Ca2+ imaging in lingual slices and examined alpha-gustducin immunoreactivity in the same cells.

44 or alpha(t-rod) confirmed the involvement of alpha-gustducin in bitter (quinine and denatonium) and s

45 ter and sweet compounds, the precise role of alpha-gustducin in bitter and sweet taste is presently u

46 direct involvement of the G-protein subunit alpha-gustducin in bitter taste transduction in taste ce

47 ntigen was present on many cells that lacked alpha-gustducin in foliate and vallate papillae.

48 mma subunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor cells.

49 eared on taste-bud cells that also expressed alpha-gustducin in the order: foliate and vallate papill

50 7) taste and demonstrated the involvement of alpha-gustducin in umami [monosodium glutamate (MSG), mo

51 A proposed mechanism for alpha-gustducin involves coupling specific cell-surface

52 These data support the hypothesis that alpha-gustducin is involved in the transduction of both

53 The alpha subunit of gustducin (alpha-gustducin) is critical for transduction of respons

54 Alpha-gustducin knock-out (KO) mice have greatly diminis

55 behavioral sensitivity to bitter stimuli in alpha-gustducin knock-out mice thus appears to be the co

56 tics, and this prevention is largely lost in alpha-gustducin-knockout mice.

57 in any of the taste responses that remain in alpha-gustducin KO mice.

58 binding regulatory proteins expressed in the alpha-gustducin lineage of taste cells mediate these res

59 Ingestion of glucose by alpha-gustducin null mice revealed deficiencies in secre

60 olated small bowel and intestinal villi from alpha-gustducin null mice showed markedly defective GLP-

61 Yet, alpha-gustducin-null mice are not completely unresponsiv

62 wild-type alpha-gustducin as a transgene in alpha-gustducin-null mice fully restored responsiveness

63 bitter and sweet taste responsiveness of the alpha-gustducin-null mice suggests that other guanine nu

64 pha-gustducin promoter in both wild-type and alpha-gustducin-null mice.

65 here was a normal number of cells expressing alpha-gustducin or the A antigen in regenerated taste bu

66 Genetic ablation of either G alpha-gustducin or TrpM5, essential elements of the T2R

67 ssed alpha-gustducin, but only a fraction of alpha-gustducin-positive cells expressed Lewis(b).

68 which transgene expression was driven by an alpha-gustducin promoter coupling BDNF expression to the

69 cin and expressed it as a transgene from the alpha-gustducin promoter in both wild-type and alpha-gus

70 Nevertheless, some taste cells lacking alpha-gustducin responded to bitter stimuli, suggesting

71 Transgenic expression of rat alpha-gustducin restored responsiveness of gustducin nul

72 d, the mutant transgene inhibited endogenous alpha-gustducin's interactions with taste receptors, i.e

73 responses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE)

74 markers neuronal cell adhesion molecule and alpha-gustducin, suggesting that both the taste receptor

75 ated Ca2+ channels, K(+)ATP channels and the alpha-gustducin taste pathway do not appear to be involv

76 The human L cell line NCI-H716 expresses alpha-gustducin, taste receptors, and several other tast

77 et and bitter taste, apparently unrelated to alpha-gustducin, that increase cyclic AMP or inositol 1,

78 al sweeteners, which act through T1R2 + T1R3/alpha-gustducin to activate PLC beta2 and PKC betaII.

79 1Rs are colocalized with each other and with alpha-gustducin, transducin or phospholipase C beta2 to

80 with reciprocal regulation of T1R2, T1R3 and alpha-gustducin versus T1R1, transducin and phospholipas

81 labeled with antibodies against Vimentin and alpha-Gustducin were easily identified and counted under

82 The G-protein subunit alpha-gustducin, which is similar to rod transducin, has