ライフサイエンスコーパス: cloning

1 deletions, site-directed mutagenesis and sub-cloning).

2 xome capture and sequencing for rapid R gene cloning.

3 i strains, to develop IVA (In Vivo Assembly) cloning.

4 c mtDNA population by means of intracellular cloning.

5 g the process of M. genitalium single-colony cloning.

6 ing varietal improvement prior to vegetative cloning.

7 the number of transformants compared with TA cloning.

8 y the madC gene was identified by positional cloning.

9 -2 and isolated the causal gene by map-based cloning.

10 ications such as B cell sequencing and/or Ab cloning.

11 ts, streamlining subsequent Ab analysis, and cloning.

12 We have validated the workflow by cloning 24 human proteins of biopharmaceutical value, ei

13 ting costs of NGS-based methods, mapping and cloning a mutation of interest has become straightforwar

14 By precisely resolving or cloning a number of these loci, we demonstrate that a br

15 admium biosensing construct was assembled by cloning a single-output cadmium biosensor element, cadRg

16 For an afternoon ligation, CG cloning achieved double the cloning efficiency and more

17 r this molecule was obtained by means of PCR cloning after MS sequencing of the protein combined with

18 automated primer design and recombinational cloning, allowing one to rapidly go from a genomic locus

19 ha-/beta-tryptase ratios were constructed by cloning alpha-and beta-tryptase PCR products to generate

20 lonal anti-ADAMTS13 autoantibodies (mAbs) by cloning an immunoglobulin G (IgG)4kappa- and IgG4lambda-

21 High-throughput sequencing and single-cell cloning analysis of the BCR repertoire revealed altered

22 Positional cloning, analysis of independent alleles, and transgenic

23 ur detector relies on instantaneous spectral cloning and a single-step, coherent field processor.

24 In this study we employed high-throughput cloning and a variety of mass spectrometry approaches to

25 9 system, are systematized for fast, modular cloning and accommodate diverse regulatory sequences to

26 Our approach requires no molecular cloning and allows a large number of cell lines to be pr

27 (5 h), cells were processed for single-cell cloning and analyzed for chromosomally integrated HHV-6A

28 A human-readable formal description of cloning and assembly strategies, which also allows for a

29 ts, making DNA libraries, as well as routine cloning and bacterial expression of genes.

30 Here, we report the cloning and brain distribution of UCN3 mRNA in a saurops

31 lete IGS sequences, obtained by conventional cloning and by Pacific Biosciences sequencing.

32 This study reports for the first time the cloning and characterization of a broad-specificity lipi

33 Here, we describe the cloning and characterization of a C. reinhardtii version

34 ts the first study describing the isolation, cloning and characterization of a full length gene encod

35 racterized; here, we describe the positional cloning and characterization of a maize (Zea mays) PISTI

36 Here we report the cloning and characterization of a new functional epiderm

37 Here we report the cloning and characterization of a quantitative trait loc

38 Here we present the identification, cloning and characterization of the gymnosperm Norway sp

39 Now, by cloning and characterizing a quantitative trait locus (Q

40 ivities of single murine IgA plasma cells by cloning and characterizing large numbers of monoclonal a

41 Cloning and electrophysiological characterization of duc

42 By cloning and expressing several TCRs isolated from unconv

43 Cross-reactivity to VZV is reconstituted by cloning and expressing TCRA/TCRB receptors from T cells

44 BAC cloning and expression analyses were employed to charact

45 vation, correction of frameshifts as well as cloning and expression of candidate methyltransferases (

46 Cloning and expression of immunoglobulin heavy and light

47 In this study, we report the cloning and expression of recombinant mouse FH and three

48 Cloning and expression of the putative AdoCbl-dependent

49 sed approach that led ultimately to the cDNA cloning and functional characterization of many of today

50 ossible involvement of MMPs was addressed by cloning and functional characterization of Sl2-MMP and S

51 By map-based cloning and functional genomic approaches, we previously

52 a [Vvi]) CCC has a role in salt tolerance by cloning and functionally characterizing the gene from th

53 netic component, and so far, only positional cloning and genomewide association studies have been use

54 Here we report the cloning and heterologous expression of the Neisseria bac

55 precursor was developed for high-throughput cloning and high expression of amiRNAs in monocots.

56 computational effort between regions through cloning and merging steps, as in the weighted ensemble m

57 sequence processing or among pyrosequencing, cloning and microscopy, while taxon identification was a

58 Cloning and molecular characterization of SCL33, a serin

59 Here we demonstrate the cloning and propagation of highly clonogenic, 'ground st

60 binding modality was generated by molecular cloning and recombinant protein expression.

61 We isolated and identified TSV by map-based cloning and rescue experiments, combined with genetic, c

62 Here, we used unbiased, high-throughput cloning and retroviral expression of individual pre-sele

63 Previous analyses of these methods relied on cloning and Sanger sequencing to evaluate their efficien

64 iously from the same samples by standard PCR cloning and Sanger sequencing, and showed that both meth

65 st R genes also validates a highly efficient cloning and screening strategy.

66 ment length polymorphism fingerprinting, and cloning and sequencing of full-length 18S rRNA genes.

67 Cloning and sequencing of the 16S rRNA gene showed domin

68 ic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) l

69 Current advances in DNA synthesis, cloning and sequencing technologies afford high-throughp

70 It progresses from genetics to cloning and sequencing to biochemistry to structural bio

71 ajor responses on ibrutinib using Sanger, TA cloning and sequencing, and highly sensitive and allele-

72 targeted and degenerate primers followed by cloning and sequencing, we determined that JNA harbors a

73 c PCR and/or Vbeta-specific PCR, followed by cloning and sequencing.

74 We isolated the Cul4 gene by positional cloning and showed that it encodes a BROAD-COMPLEX, TRAM

75 technologies that do not depend on molecular cloning and that produce very long reads.

76 tome analysis, full-length complementary DNA cloning, and characterization of expressed P450 proteins

77 efficiency of marker development, map-based cloning, and comparative genomics in barley and related

78 s, long-range polymerase chain reaction with cloning, and complementary DNA sequencing were used to c

79 sis, enzyme assays with WRC tissue extracts, cloning, and functional characterization of cytochrome P

80 romatin immunoprecipitation (ChIP), promoter cloning, and site-directed mutagenesis, real-time quanti

81 odies (hmAb) against gliadin are produced by cloning antibody genes from single IgA-producing plasma

82 This advance was achieved with a multistep cloning approach and by using DNA dilutions to improve p

83 We adopted a map-based cloning approach and TALE-based technology to isolate an

84 A positional cloning approach indicates that the mutation in aura is

85 Taking the positional cloning approach, the Chr.9_ClpP5 gene, one of the prote

86 Using a high-resolution map-based cloning approach, we delimited Htn1 to a 131.7-kb physic

87 onstitutes a platform for synteny-based gene cloning approaches and can support the forthcoming white

88 multaneously, we established simple one-step cloning approaches for expression of multiple sgRNAs wit

89 and fine-tuned to account for sequencing and cloning artifacts.

90 approach require manipulation via molecular cloning as well as in vitro transcription.

91 using purified enzymes for multiple-fragment cloning as well as mutagenesis is gaining ground.

92 This work represents the first direct cloning based heterologous expression of natural product

93 resonance energy transfer-based interaction cloning (BRIC), a screening strategy that can detect pro

94 occur not only during isolation prior to BAC cloning but also when virus is regenerated.

95 We used a positional cloning-candidate gene approach to identify molecular ba

96 ers, chemical induction, and high-throughput cloning capabilities.

97 e progenitors are characterized by increased cloning capacity, impaired development into mature cells

98 d provide proof of principle for therapeutic cloning combined with cell therapy.

99 ne mapping of quantitative traits, map-based cloning, comparative mapping, and in marker-assisted whe

100 Antibody cloning confirmed elicitation of high levels of somatic

101 quencing (or "Cos-Seq"), based on functional cloning coupled to next-generation sequencing.

102 ue to low efficiencies and the high costs of cloning domestic species, only a limited number of ident

103 dissociation-induced apoptosis and increased cloning efficiency (2-3-fold versus Y-27632) without aff

104 oon ligation, CG cloning achieved double the cloning efficiency and more than 4-fold the number of tr

105 ts hPSC survival upon dissociation; however, cloning efficiency is often still low.

106 The essence behind its high cloning efficiency is the extreme toxicity and small siz

107 e cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold.

108 iciency and a 96 +/- 4% postsort single-cell cloning efficiency.

109 By cloning eight A. lyrata homologs and complementing them

110 Here, we describe the cloning, engineering and production in plants of a candi

111 mal DNA was also sensitive to restriction in cloning experiments.

112 This study describes the cloning, expression and purification of hypoxanthine-gua

113 Here, we report the cloning, expression, and characterization of the Rab1 ho

114 After cloning, expression, and purification of the correspondi

115 Homology-based cloning facilitated characterization of a functional P.

116 this molecular toolkit allows combinatorial cloning for multiple transgene expression in seeds, vege

117 Here, we report a cloning-free method to target the mouse genome by pronuc

118 We describe a cloning-free single-guide RNA (sgRNA) synthesis, coupled

119 r this pipeline, including target selection; cloning-free single-guide RNA (sgRNA) synthesis; microin

120 we employ CRISPR/Cas9 technology to build a cloning-free toolkit that addresses commonly encountered

121 We conclude that the original concerns that cloning had caused early-onset OA in Dolly were unfounde

122 Over the last four decades, molecular cloning has evolved tremendously.

123 Animal cloning has gained popularity as a method to produce gen

124 In tomato (Solanum lycopersicum), molecular cloning has revealed that the underlying genes of natura

125 on holds the potential for optimal molecular cloning, however, current strategies require specialised

126 Map-based cloning identified that the LHR1 gene encodes the polyam

127 recessive genes were involved, and map-based cloning identified two homologous genes in duplicated re

128 ad overview of the historical development of cloning in domestic animals, of its application to the p

129 ovides a key step for application of quantum cloning in quantum information science.

130 other columnar epithelia collectively resist cloning in their elemental states.

131 Streptococcus sanguinis, we have developed a cloning-independent methodology, which uses a countersel

132 nce of the two oligonucleotides required for cloning into 'B/c' compatible vectors.

133 material, HCMV genomes can be stabilized by cloning into bacterial artificial chromosomes (BACs), an

134 and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error

135 be an improved version in which target mimic cloning is achieved by annealing two partly-overlapping

136 TPA cloning is scarless and sequence independent.

137 this article, we show that the analogous "CG cloning" is faster and far more efficient, using AhdI to

138 P) mutation on NK cell cytolytic function by cloning it into a lentiviral expression vector prior to

139 een assembled together by modular idempotent cloning, it is unclear if such simplified strategies sca

140 sive substrate screen by in vitro expression cloning (IVEC).

141 igene expression due to increased labour for cloning, limited vector capacity, requirement of multipl

142 The realization of a cloning machine with built-in quantum memory provides a

143 Rapid amplification of cDNA ends cloning mapped transcription start sites adjacent to the

144 Our in vivo blunt-end cloning method and destabilization-domain-fused Cas9 var

145 lyzed the HBV precore/core gene sequences by cloning method in 33 chronic HBV-infected patients durin

146 cant advantages over alternative sorting and cloning methods by eliminating the necessity for repetit

147 Traditional cloning methods have limitations on the number of DNA fr

148 ecently developed single-cell-based antibody cloning methods have uncovered a new generation of far m

149 f cells and organisms, relying upon cellular cloning methods that remain unchanged for decades, are l

150 ation in molecular biology, from traditional cloning methods to modern synthetic biology and molecula

151 rovement in sensitivity compared to previous cloning methods while maintaining reproducible identific

152 ventors, developers and adopters of Type IIS cloning methods.

153 bly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, com

154 n vitro isothermal reactions are useful when cloning multiple fragments, for site-directed mutagenesi

155 and populations, this study will support the cloning of a major yield QTL on chromosome 3B that is hi

156 We report the cloning of a P. pachyrhizi resistance gene CcRpp1 (Cajan

157 in this study will underpin fine-mapping and cloning of agronomically important genes and the assembl

158 is challenging question, we report the first cloning of an S. japonicum aquaglyceroporin (SjAQP) from

159 It has been 20 years since the cloning of ATR, the last of the three to be identified.

160 rminants in the blast fungus resulted in the cloning of avirulence genes PWT3 and PWT4, whose gene pr

161 as successfully tested for the synthesis and cloning of both prokaryotic and eukaryotic genes through

162 Recent studies reported cloning of candidate genes for several diseases in cucum

163 y of these events, however, has hindered the cloning of cells with the desired rearrangement before o

164 rt the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reacti

165 Cloning of cZNF292 validated the predicted back splicing

166 hance the human genetic endowment by genetic cloning of eminent individuals is not warranted.

167 also used endpoint dilution PCR followed by cloning of env genes to create pseudotyped virus to expl

168 Map-based cloning of fcd1 indicated that this gene encodes the pla

169 Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3.

170 accurate and cost effective fabrication and cloning of gene-length sequences that employ the widely

171 sed significant progress in fine-mapping and cloning of genes controlling QDR.

172 thesis, combinatorial assembly and cell-free cloning of genes, respectively.

173 thod that allows for the rapid and efficient cloning of gRNA pairs into expression vectors.

174 e, from the time of human vaccination to the cloning of heavy- and light-chain genes.

175 l cumbersome as it requires the synthesis or cloning of homology templates.

176 With this new system, cloning of inducible constructs is no longer limited to

177 Through systematic cloning of its genes, we identified an Acr solely respon

178 We report here the cloning of Male Sterile23 (Ms23), encoding an anther-spe

179 A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCN

180 The cloning of Ms2 has substantial potential to assemble pra

181 Cloning of multiple genes in a single vector has greatly

182 refractory to transformation or genome-wide cloning of open reading frames.

183 Here we report the map-based cloning of qHSR1 and the molecular mechanism of qHSR1-me

184 We report an experiment that realizes cloning of quantum states from an electron spin to a nuc

185 Cloning of RDO5 showed that it encodes a PP2C phosphatas

186 This work ushered in the cloning of related proteins, GATA-2-6, with distinct and

187 Cloning of rye reveals that it encodes a nicotinic acety

188 holipid biosynthesis in Escherichia coli and cloning of several of the genes encoding these enzymes i

189 ion experiments were performed by sequential cloning of six P. falciparum isolates growing in human e

190 sment of their pathogenic potential requires cloning of such antibodies.

191 Cloning of the B. subtilis epsH-K genes into Escherichia

192 Here we report cloning of the biosynthetic gene cluster for the beta-la

193 hern bobwhite myoglobin were deduced by cDNA cloning of the coding sequence from mRNA.

194 Since the cloning of the critical adapter, LAT (linker for activat

195 search on elicitins culminated in the recent cloning of the elicitin response (ELR) cell surface rece

196 Amplification and cloning of the full-length of the sodium channel gene in

197 Cloning of the gene in an expression vector for ex vivo

198 Here, we report positional cloning of the gene responsible for production of retrov

199 The cloning of the H3R cDNA in 1999 by Lovenberg et al. allo

200 In our 1998 JCI paper, we reported the cloning of the human nuclear receptor PXR and demonstrat

201 Here, we report the identification and cloning of the M. truncatula NFS2 gene that regulates th

202 m of this study was the characterization and cloning of the major allergen from this hamster.

203 We herein report the cloning of the Medicago truncatula NFS1 gene that regula

204 Here, we describe the map-based cloning of the Ms2 gene and show that Ms2 confers male s

205 Here we report the map-based cloning of the Ms2 gene.

206 asochistic endeavor) in 1987, leading to the cloning of the Pemt cDNA.

207 typicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes.

208 Positional cloning of the SIS8 gene revealed that it encodes a puta

209 Here, we describe the cloning of two paralog genes, H03-IPSE and H06-IPSE, whi

210 via next-generation approaches and the rapid cloning of viral genes are critical for basic research,

211 ext-generation sequencing for the positional cloning of zebrafish mutations has increased the number

212 Here, we report cloning of ZmNope1 on the basis of synteny with rice.

213 f phytoremediating plants based on dedicated cloning or breeding programs.

214 to overcome the limitations associated with cloning ORFs from a genome that remains incompletely ann

215 ersatile positive-selection vector ideal for cloning PCR products, making DNA libraries, as well as r

216 /384-well plates, Petri dishes, or vials for cloning, PCR, and other single-cell biochemical assays.

217 were characterized by direct sequencing and cloning; phenotypes were assessed by assays of rod opsin

218 After validating this direct cloning "plug-and-play" approach with surfactin, we gene

219 advantages over alternative methods for all cloning procedures (insertions, deletions, site-directed

220 enzymatic assembly and reduces all molecular cloning procedures to a single-tube, single-step PCR, pe

221 originating from standard cDNA synthesis and cloning procedures.

222 lows unprecedented simplification of complex cloning procedures: five simultaneous modifications of a

223 the application of molecular biology allowed cloning, production and characterization of hundreds of

224 plasmids from grant-funded, high-throughput cloning projects performed in our laboratory, plasmids f

225 ntum no-cloning theorem, approximate quantum cloning protocols have received wide attention with rema

226 ivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis).

227 Additionally, the new spectral-cloning receiver can potentially intercept communication

228 Nevertheless, methods such as cloning, recombination, and electrospinning have not suc

229 Positional cloning revealed a nonsense mutation of tln1 in this mut

230 Here, a single cell cloning revealed that HepAD38 cells, a widely-used HBV-i

231 Positional cloning revealed that rim1 encodes an allele of AtICln (

232 Map-based cloning revealed that the Abph2 mutation was caused by t

233 Molecular cloning revealed that this phenotype is caused by an ele

234 Using molecular cloning, RT-PCR, Western blotting, immunolocalization an

235 gh positive-selection of recombinants in DNA cloning seems to be superior to blue/white selection bas

236 The easy cloning, selection and production, together with the dem

237 ows for specification of forward and reverse cloning sequences (for downstream ligation) and also pro

238 s consortium combines RT-PCR, exon scanning, cloning, sequencing and relative semi-quantification to

239 pared the results with those obtained by PCR-cloning-sequencing (PCR-CS).

240 The cloning-sequencing analysis, however, showed significant

241 Positional cloning showed that MAS2 encodes the putative ortholog o

242 Antibody cloning shows that donors with high ZIKV neutralizing an

243 of medical interest, it has eluded molecular cloning since its discovery, and the gene that codes for

244 n inactivation of killin within the multiple cloning site via either blunt- or sticky-end ligation no

245 due to its high background, lack of multiple cloning sites, and inability to express the genes of int

246 part of a sequence- and ligation-independent cloning (SLIC) reaction and then transformed into Escher

247 lity to generate such constructs in a single cloning step.

248 the utility of ssODNs and eliminate in vitro cloning steps for template construction.

249 simplifies both the planning and sharing of cloning strategies and is especially useful for complex

250 and PCR-independent Golden Gate and Gateway cloning strategies, which will facilitate transcriptiona

251 Using a positional cloning strategy and a retrotransposon insertion line, w

252 Using a single-cell cloning strategy coupled with microarray analysis of dif

253 t inhibition of transcription and the simple cloning strategy described in this work, CRISPR interfer

254 A cloning strategy expressed as a pydna script provides a

255 We also developed a lentiviral vector and cloning strategy to generate high-complexity pooled dual

256 Here, using a positional cloning strategy we identified variants of PROTEIN DISUL

257 Using a positional cloning strategy, we identified a missense mutation in R

258 es and five genes supported by previous gene cloning studies in maize, rice, and Arabidopsis.

259 Map-based cloning studies revealed that CTL1 mutations alter the i

260 y genetic variants in post-GWAS or post-QTL- cloning studies.

261 were supported by both QTL analyses and gene cloning studies.

262 Merging the 2in1 cloning system with new-generation FRET fluorophore pair

263 the benefit of the recombination-based 2in1 cloning system with the latest state-of-the-art fluoresc

264 or cell population was performed by the ring-cloning technique.

265 The emergence of molecular cloning techniques and identification of fluorophores li

266 mented by a student with experience in basic cloning techniques.

267 ropose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clon

268 Cloning the entire B. subtilis epsHIJK locus into pga-de

269 ssion in mouse models and by difficulties in cloning the full-length RPGR-ORF15 cDNA that includes a

270 urther defined the ATF4-CHAC1 interaction by cloning the human CHAC1 promoter upstream of a luciferas

271 Cloning the Rj4 gene is the first step in understanding

272 Cloning the sigma2 receptor resolves a longstanding myst

273 covery of oncogenes, the advent of molecular cloning, the search for human cancer viruses, and the di

274 As a way to circumvent the quantum no-cloning theorem, approximate quantum cloning protocols h

275 As a direct consequence of the no-cloning theorem, the deterministic amplification as in c

276 . coli self-assembly have greatly simplified cloning, these have not yet been harnessed for the high-

277 We employed molecular cloning to examine how PTEN's stability, subcellular loc

278 for a wide range of applications from basic cloning to genome engineering.

279 s were used for somatic nuclear transfer and cloning to produce a bovine fetus homozygous for the Q(5

280 However, mutagenized cultured cells require cloning to yield homogeneous populations, and the limite

281 gation not only serves as a highly efficient cloning trap, but also may allow any cloned genes to be

282 ort the creation of a new positive-selection cloning vector dubbed pKILLIN, which overcomes all of th

283 ies, we constructed a new TAR-BRV- tTA(VP64) cloning vector that allows a selective isolation of a ge

284 ing an ampicillin resistance gene (bla) from cloning vectors was not detected in either the wastewate

285 d-specific RNA interference gene-suppression cloning vectors were also constructed for silencing of e

286 nomena include the following: human-mediated cloning via artificial nuclear transfer; intergeneration

287 the S-locus on chromosome 1, but positional cloning was hampered by low recombination frequency.

288 Using map-based cloning we positioned the her2 mutation to the At5g63620

289 Using map-based cloning, we delimited a candidate region including two l

290 After the HAT-selection and cloning, we established nine hybridoma clones secreting

291 Using molecular cloning, we have identified three separate domains of UL

292 Using expression cloning, we identified an accessory protein, 17 kDa memb

293 By using single-cell cloning, we identified genes that are associated with hi

294 By positional cloning, we identify the mutation in this strain as a re

295 Using mapped-based cloning, we identify the RAA7 locus, which encodes a pio

296 In the future, therapeutic cloning will bring enhanced possibilities for organ tran

297 ble the TALEN genes by combining golden gate cloning with modified constructs from the FLASH protocol

298 antibody variable genes and accelerates the cloning workflow.

299 In addition to cloning wud, we identified synaptojanin 1 (synj1) as the

300 In conclusion, by cloning Zfhx3(Sci), we have uncovered a circadian transc