ライフサイエンスコーパス: gustducin

1 icient in the gustducin alpha-subunit (alpha-gustducin).

2 mmunoreactivity to an antibody against alpha-gustducin.

3 a bitter-responsive receptor that activates gustducin.

4 s and the tastant-associated G protein alpha-gustducin.

5 sucralose internalized T1R2, T1R3 and alpha-gustducin.

6 ptor antagonist lactisole or siRNA for alpha-gustducin.

7 ussis toxin and by genetic deletion of alpha-gustducin.

8 ngual taste cells and strongly express alpha-gustducin.

9 t not in knockout mice lacking T1R3 or alpha-gustducin.

10 Mouse intestinal L cells also express alpha-gustducin.

11 all, bitter-responsive cells expressed alpha-gustducin.

12 ing the molecular markers Vimentin and alpha-Gustducin.

13 (SNAP-25), and to a lesser extent with alpha-gustducin.

14 reduced by 70% in mutant mice lacking alpha-gustducin.

15 antigen, whether or not they expressed alpha-gustducin.

16 ate specific tastant-dependent activation of gustducin, a G protein implicated in bitter signaling.

17 press T2R "bitter-taste" receptors and alpha-gustducin, a G protein involved in chemosensory transduc

18 subset of type II cells that contains alpha-gustducin, a G-protein involved in bitter transduction,

19 butions of these antigens with that of alpha-gustducin, a G-protein subunit implicated in responses t

20 A key molecule, alpha-gustducin, a primarily taste-specific G protein alpha-su

21 Alpha-gustducin, a taste cell-expressed G-protein alpha subuni

22 Gustducin, a taste-specific G-protein closely related to

23 Gustducin, a transducin-like guanine nucleotide-binding

24 eins derived from visual transducin or taste gustducin alpha subunits, but no other Galpha HD protein

25 ing and characterizing mice deficient in the gustducin alpha-subunit (alpha-gustducin).

26 weet and amino acid taste receptors, and the gustducin alpha-subunit GNAT3 leads to male-specific ste

27 The alpha subunit of gustducin (alpha-gustducin) is critical for transduction

28 Signaling components included gustducin-alpha and Galphao, but not rod or cone transdu

29 Gustducin-alpha resembles transducin-alpha functionally

30 We set out to determine whether alpha-gustducin also mediates umami taste and whether rod alph

31 Umami detection involving alpha-gustducin and alpha(t-rod) occurs in anteriorly placed t

32 Both alpha-gustducin and alpha-transducin activate phosphodiesteras

33 generated a dominant-negative form of alpha-gustducin and expressed it as a transgene from the alpha

34 Both Galpha gustducin and Galpha transducin are involved in umami si

35 entiated light cells that also express alpha-gustducin and may be involved in intercellular interacti

36 onical signaling components (i.e., G-protein gustducin and phospholipase C beta2).

37 nds on an interaction with the C terminus of gustducin and requires G-protein betagamma subunits to p

38 Gustducin and rod transducin, which is also expressed in

39 were incubated with antibodies against alpha-gustducin and the human blood group A antigen.

40 Thus, proposed models for alpha-gustducin and those found by other laboratories may be p

41 east two varieties: those immunoreactive for gustducin and those immunoreactive for PGP 9.5.

42 Gustducin and transducin are activated in the presence o

43 pressed in bovine taste tissue and that both gustducin and transducin, in the presence of bovine tast

44 eptors are coupled through G-proteins, alpha-gustducin and transducin, to activate phospholipase C be

45 taste transduction cascade involving Galpha-gustducin and transient receptor potential melastatin 5.

46 s of single and double KO mice lacking alpha-gustducin and/or alpha(t-rod) confirmed the involvement

47 a2 (PLCbeta2) or the G-protein subunit alpha-gustducin, and serotonin (5HT) as markers of type I, II,

48 s sweet taste receptors, the taste G protein gustducin, and several other taste transduction elements

49 s sweet taste receptors, the taste G protein gustducin, and several other taste transduction elements

50 Both the gustatory G-protein, alpha-gustducin, and the cell-surface carbohydrate, the A bloo

51 nd their associated heterotrimeric G protein gustducin are involved in sugar and amino acid sensing i

52 al and genetic studies have implicated alpha-gustducin as a key component in the transduction of both

53 l experiments, expression of wild-type alpha-gustducin as a transgene in alpha-gustducin-null mice fu

54 All Lewis(b)-positive cells expressed alpha-gustducin, but only a fraction of alpha-gustducin-positi

55 the activation in response to denatonium of gustducin by presumptive bitter-responsive receptors pre

56 tivity should be reflected in the numbers of gustducin-containing cells in different taste bud popula

57 Taste cells with alpha-gustducin could express either presynaptic proteins or t

58 troduced into the C-terminal region of alpha-gustducin critical for receptor interaction rendered the

59 eceptor subunit T1R3 and the taste G protein gustducin, expressed in enteroendocrine cells, underlie

60 ssion was found exclusively in the G-protein gustducin-expressing bitter receptor cells, while TNF wa

61 fungiform taste buds contained twice as many gustducin-expressing cells (6.8/taste bud) as those of t

62 mal vallate papilla had a mean of 8.37 alpha-gustducin-expressing cells and 5.22 A-expressing cells p

63 Unexpectedly, all gustducin-expressing cells lacked voltage-gated Ca(2+) c

64 t family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and

65 cin Galpha (Galphat) and the closely related gustducin (Galphag), but not Galphai1, Galphas, or Galph

66 Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated by taste cell

67 from the upstream region of the mouse alpha-gustducin gene acts as a fully functional promoter to ta

68 ving that the targeted deletion of the alpha-gustducin gene caused the taste deficits of the null mic

69 h "knock-out" animals deficient in the alpha-gustducin gene clearly demonstrate that gustducin is an

70 hancer from the distal portion of the murine gustducin gene that, in combination with the minimal pro

71 with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) a

72 Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells profile

73 Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13

74 We conclude that gustducin heterotrimers transduce responses to bitter an

75 Although gustducin-immunoreactive taste cells appear similar in o

76 imaging in lingual slices and examined alpha-gustducin immunoreactivity in the same cells.

77 The ultrastructural distribution of gustducin immunoreactivity is consistent with its propos

78 lls that contain the G protein alpha subunit gustducin, implying that they function as gustducin-link

79 ha(t-rod) confirmed the involvement of alpha-gustducin in bitter (quinine and denatonium) and sweet (

80 d sweet compounds, the precise role of alpha-gustducin in bitter and sweet taste is presently unclear

81 t involvement of the G-protein subunit alpha-gustducin in bitter taste transduction in taste cells ha

82 was present on many cells that lacked alpha-gustducin in foliate and vallate papillae.

83 mmunoreactivity to antisera directed against gustducin in taste buds of rat circumvallate papilla.

84 NF expression to the postnatal expression of gustducin in taste cells.

85 bunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor cells.

86 on taste-bud cells that also expressed alpha-gustducin in the order: foliate and vallate papillae > n

87 te and demonstrated the involvement of alpha-gustducin in umami [monosodium glutamate (MSG), monopota

88 ng paper, we demonstrate that T2Rs couple to gustducin in vitro, and respond to bitter tastants in a

89 t compounds, and to elicit the nature of the gustducin-independent pathways, we generated a dominant-

90 The taste receptor-gustducin interaction can be competitively inhibited by

91 A proposed mechanism for alpha-gustducin involves coupling specific cell-surface recept

92 Our results suggest that gustducin is a principal mediator of both bitter and swe

93 Gustducin is a taste receptor cell (TRC)-specific G prot

94 Gustducin is a transducin-like G protein (guanine nucleo

95 Gustducin is a transducin-like G protein selectively exp

96 lpha-gustducin gene clearly demonstrate that gustducin is an essential molecule for tasting certain b

97 Gustducin is believed to be involved in bitter and possi

98 In this paper, we show that gustducin is expressed in bovine taste tissue and that b

99 These data support the hypothesis that alpha-gustducin is involved in the transduction of both sweet-

100 The alpha subunit of gustducin (alpha-gustducin) is critical for transduction of responses to

101 Alpha-gustducin knock-out (KO) mice have greatly diminished, b

102 ioral sensitivity to bitter stimuli in alpha-gustducin knock-out mice thus appears to be the conseque

103 and this prevention is largely lost in alpha-gustducin-knockout mice.

104 of the taste responses that remain in alpha-gustducin KO mice.

105 g regulatory proteins expressed in the alpha-gustducin lineage of taste cells mediate these responses

106 it gustducin, implying that they function as gustducin-linked receptors.

107 cted results with knockout mice suggest that gustducin may be directly involved in both bitter and sw

108 To gain insights into how gustducin mediates responses to bitter and sweet compoun

109 The gustducin minimal 1.4 kb promoter (GUS(1.4)) by itself w

110 patterns of GAD with the TRC protein markers gustducin, neural cell adhesion molecule, protein gene p

111 hape to the pyriform PGP 9.5/NSE population, gustducin never colocalizes with PGP 9.5 or NSE.

112 ed small intestine and intestinal villi from gustducin null mice displayed markedly defective glucago

113 Ingestion of glucose by alpha-gustducin null mice revealed deficiencies in secretion o

114 small bowel and intestinal villi from alpha-gustducin null mice showed markedly defective GLP-1 secr

115 t alpha-gustducin restored responsiveness of gustducin null mice to both bitter and sweet compounds,

116 Yet, alpha-gustducin-null mice are not completely unresponsive to b

117 type alpha-gustducin as a transgene in alpha-gustducin-null mice fully restored responsiveness to bit

118 and sweet taste responsiveness of the alpha-gustducin-null mice suggests that other guanine nucleoti

119 stducin promoter in both wild-type and alpha-gustducin-null mice.

120 ivity for the taste cell-specific G protein, gustducin, occurs in a subset ofrENaC positive taste cel

121 as a normal number of cells expressing alpha-gustducin or the A antigen in regenerated taste buds; in

122 Knockout mice that lack gustducin or the sweet taste receptor subunit T1r3 have

123 There was no evidence for the activation of gustducin or transducin in the beta-cell.

124 Genetic ablation of either G alpha-gustducin or TrpM5, essential elements of the T2R transd

125 a common second-messenger cascade involving gustducin, phospholipase C beta2, and the transient rece

126 bitter taste signaling cascade (i.e., TAS2R-gustducin-phospholipase Cbeta [PLCbeta]- inositol 1,4,5-

127 ste buds of the fungiform papillae had fewer gustducin-positive cells (3.1/taste bud) than those of o

128 lpha-gustducin, but only a fraction of alpha-gustducin-positive cells expressed Lewis(b).

129 Gustducin-positive cells were seen in all taste bud regi

130 r to target lacZ transgene expression to the gustducin-positive subset of taste receptor cells (TRCs)

131 transgene expression was driven by an alpha-gustducin promoter coupling BDNF expression to the postn

132 d expressed it as a transgene from the alpha-gustducin promoter in both wild-type and alpha-gustducin

133 compounds, demonstrating the utility of the gustducin promoter.

134 fluorescent protein under the control of the gustducin promoter.

135 Nevertheless, some taste cells lacking alpha-gustducin responded to bitter stimuli, suggesting that o

136 Transgenic expression of rat alpha-gustducin restored responsiveness of gustducin null mice

137 mutant transgene inhibited endogenous alpha-gustducin's interactions with taste receptors, i.e., it

138 nses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE) and Gb

139 Here we investigate gustducin's role in taste transduction by generating and

140 rs neuronal cell adhesion molecule and alpha-gustducin, suggesting that both the taste receptor cells

141 a2+ channels, K(+)ATP channels and the alpha-gustducin taste pathway do not appear to be involved.

142 e human L cell line NCI-H716 expresses alpha-gustducin, taste receptors, and several other taste sign

143 bitter taste, apparently unrelated to alpha-gustducin, that increase cyclic AMP or inositol 1,4,5 tr

144 eteners, which act through T1R2 + T1R3/alpha-gustducin to activate PLC beta2 and PKC betaII.

145 e colocalized with each other and with alpha-gustducin, transducin or phospholipase C beta2 to differ

146 ssbred with green fluorescent protein (GFP) (gustducin) transgenic mice.

147 eciprocal regulation of T1R2, T1R3 and alpha-gustducin versus T1R1, transducin and phospholipase C be

148 from the GUS(8.4) promoter and of endogenous gustducin was coordinately lost after nerve section and

149 d with antibodies against Vimentin and alpha-Gustducin were easily identified and counted under a lig

150 The G-protein subunit alpha-gustducin, which is similar to rod transducin, has been