ライフサイエンスコーパス: mecA gene

1 to oxacillin (based on amplification of the mecA gene).

2 ssette chromosome (SCC) elements without the mecA gene.

3 e methicillin resistant and positive for the mecA gene.

4 the results of PCR for the detection of the mecA gene.

5 studied by LightCycler PCR for the sa442 and mecA genes.

6 s-specific (nuc) and methicillin resistance (mecA) genes.

7 acilities were analyzed for the conventional mecA gene and mecC homologue by using standard PCR-based

8 ntification was based on the presence of the mecA gene and S. aureus-specific femA-SA gene, with abse

9 c sensitivity was 1 CFU per reaction for the mecA gene and was 1 to 250 CFU per reaction depending on

10 had virtually identical DNA sequences in the mecA gene and were similar in genetic organization in th

11 time PCR assays against novel targets in the mecA gene as an adjunct to routine susceptibility testin

12 were evaluated by using the presence of the mecA gene, as detected by PCR, as the "gold standard." T

13 Only one CoNS isolate with mecA gene-associated resistance was not detected by usin

14 of bacteremia from isolates that carried the mecA gene but were susceptible to oxacillin (oxacillin-s

15 icrodilution method and the detection of the mecA gene by PCR ("gold standard" reference result) were

16 crodilution method, and the detection of the mecA gene by PCR were compared with the commercial produ

17 eptible to oxacillin and did not contain the mecA gene by Southern blot hybridization.

18 However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensiti

19 to those generated by two reference methods: mecA gene detection and MICs of oxacillin previously det

20 ese methods were compared to the results for mecA gene detection by a PCR method.

21 for 48 h (one isolate that did not carry the mecA gene did not grow, and the sensitivity was 100%).

22 to the genus level and the detection of the mecA gene directly from a positive blood culture bottle.

23 enicillin-binding protein 2A (encoded by the mecA gene), directly contributes to pathogenicity during

24 lture, of which two demonstrated evidence of mecA gene dropout.

25 SA0140 (SA) and the methicillin resistance (mecA) gene employing standard TaqMan chemistries.

26 esistance arose around this period, when the mecA gene encoding methicillin resistance carried on an

27 CCmec), the genetic element that carries the mecA gene encoding methicillin resistance.

28 eus is dependent upon the acquisition of the mecA gene encoding penicillin-binding protein 2a (PBP2a)

29 The detection of the mecA gene for 69 blood cultures with only S. aureus or S

30 strains of S. aureus that have acquired the mecA gene for PBP2a are designated as methicillin-resist

31 tion of plasmids containing known amounts of mecA gene fragments.

32 The acquisition of the mecA gene from an unknown origin imparted S. aureus with

33 trip) was developed for the detection of the mecA gene from methicillin-resistant Staphylococcus aure

34 ce analysis of a 2.1-kb gene fragment of the mecA gene from the susceptible isolate revealed a one-ba

35 The mecA gene homolog and regions flanking it were cloned an

36 ylococcus aureus (MRSA) encoding a divergent mecA gene in 2011 was highly significant.

37 hybridization assay was used to test for the mecA gene in 416 clinical staphylococcal isolates.

38 Identification of the mecA gene in 599 cultures containing S. aureus or S. epi

39 mary culture to the time to detection of the mecA gene in S. aureus isolates.

40 ism 7700 Sequence Detector for detecting the mecA gene in various species of staphylococci.

41 PCR detected the methicillin resistance (mecA) gene in all three culture-confirmed methicillin-re

42 ologue of the acquired Staphylococcus aureus mecA gene is present as a native gene in Staphylococcus

43 Real-time PCR testing for the mecA gene (mecA PCR), which confers methicillin resistan

44 We synthesized, cloned, and expressed the mecA gene of S. sciuri in Escherichia coli, and the prot

45 attempt to activate the apparently "silent" mecA gene of S. sciuri, a methicillin-resistant derivati

46 The mecA genes of strains K1 and K11 and one of the two copi

47 Staph ID/R agreed with mecA gene PCR for all samples and agreed with rpoB/16S r

48 cing for species identity determinations and mecA gene PCR to confirm mecA gene results.

49 el electrophoretic pattern, carried the same mecA gene polymorph type II, were free of the transposon

50 he major MRSA clone except that they carried mecA gene polymorph type III.

51 tection of a clinically relevant target, the mecA gene present in methicillin-resistant Staphylococcu

52 strains, 1 strain was unable to express the mecA gene product after induction and was not included i

53 8 kbp and 0.23 kbp DNA fragments of femB and mecA genes, respectively.

54 determinations and mecA gene PCR to confirm mecA gene results.

55 or bacterial and Staphylococcus 16S rRNA and mecA gene sequences.

56 ts were resolved by rpoB gene sequencing and mecA gene sequencing, respectively.

57 iated pneumonia and can be identified by the mecA gene that confers resistance.

58 ant, while introduction of the plasmid-borne mecA gene, the genetic determinant of the beta-lactam re

59 In the presence of the mecA gene, the probe is cut and no test line is formed o

60 In the absence of the mecA gene, the uncut probe is bound to an antifluorescei

61 test for detection of MRSA, with PCR for the mecA gene used as the "gold standard" assay.

62 The mecA gene was absent.

63 The mecA gene was cloned and expressed in Escherichia coli,

64 The mecA gene was detected in all three by PCR; one isolate

65 bility test results and the detection of the mecA gene was observed for Staphylococcus aureus, S. epi

66 tes with oxacillin resistance related to the mecA gene were detected when 0 or 2% NaCl agar supplemen

67 y 100% of the CoNS isolates that carried the mecA gene were identified.

68 PBP2a is encoded by the mecA gene, which is carried on a distinct mobile genetic

69 The mecA gene, which refers resistant to methicillin, was de

70 heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplificat